Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Plasma Modified 2D and 3D PCL Scaffolds
2.2. PCL Scaffold Characterization
2.3. Standard Cell Culture
2.4. Indirect Cell Viability Assay
2.5. Cell Proliferation and Attachment Assays by ELISA
2.6. Observation of Cellular Attachment under Scanning Electron Microscopy (SEM)
2.7. Gene Expression by Semi-Quantitative Polymerase Chain Reaction (PCR)
2.8. Statistical Analysis
3. Results
3.1. Physicochemical Properties of 2D and 3D PCL Scaffolds
3.2. Differences in Cell Viability, Attachment, and Proliferation on 2D and 3D Scaffolds
3.3. Attached-Cell Morphology on Different Substrates
3.4. Shifts in Apoptosis/Necrosis-Related and Pluripotency/Differentiation-Related Gene Expression on Different Substrates
3.4.1. Apoptosis/Necrosis-Related Genes
3.4.2. Pluripotency/Differentiation-Related Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Gene | Accession Number | Sequence |
---|---|---|
caspase 8 | NM_001228.4 | F: 5′-TGCAGGGGCTTTGACCACGA-3′ R: 5′-TGGGGGCCTCCTGTCCATCA-3′ |
bcl-2 | NM_000633.2 | F: 5′-TGTGGCCTTCTTTGAGTTCG-3′ R: 5′-TCACTTGTGGCTCAGATAGG-3′ |
Apaf-1 | NM_013229.2 | F: 5′-TGGCCAGTGCCAAGATGCACA-3′ R: 5′-CGACCTCCTGCTTGGCCTGC-3′ |
PARP-1 | NM_017915.3 | F: 5′-AATCTCCAGGGGGTAGAACT-3′ R: 5′-CAGAGCCTGTTGAAGTTGTG-3′ |
SSEA-4 | NM_203289.4 | F: 5′-GCCCTAGAACTCCAATCACA-3′ R: 5′-CCCAGATGGTATTGGACACA-3′ |
NES | NM_006617.1 | F: 5′-TCCTGCTCGCTCTCTACTTT-3′ R: 5′-CCCAGATGGTATTGGACACA-3′ |
COL2A1 | NM_001844.4 | F: 5′-CCCATTGGTCCTTGCATTAC-3′ R: 5′-GTCCTCTGCGACGACATAAT-3′ |
PPAR-2 | NM_138712.3 | F: 5′-GCATTATGAGACATCCCCACT-3′ R: 5′-CCTATTGACCCAGAAAGCGAT-3′ |
GAPDH | NM_002046.4 | F: 5′-TGCTGGCGCTGAGTACGTCG-3′ R: 5′-TGACCTTGGCCAGGGGTGCT-3′ |
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Inthanon, K.; Janvikul, W.; Ongchai, S.; Chomdej, S. Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds. J. Funct. Biomater. 2019, 10, 52. https://doi.org/10.3390/jfb10040052
Inthanon K, Janvikul W, Ongchai S, Chomdej S. Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds. Journal of Functional Biomaterials. 2019; 10(4):52. https://doi.org/10.3390/jfb10040052
Chicago/Turabian StyleInthanon, Kewalin, Wanida Janvikul, Siriwan Ongchai, and Siriwadee Chomdej. 2019. "Intrinsic Cellular Responses of Human Wharton’s Jelly Mesenchymal Stem Cells Influenced by O2-Plasma-Modified and Unmodified Surface of Alkaline-Hydrolyzed 2D and 3D PCL Scaffolds" Journal of Functional Biomaterials 10, no. 4: 52. https://doi.org/10.3390/jfb10040052