A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Synthesis and Characterization of BisBAL NPs
2.2. Fabrication of Tecoflex™ EMs and Coating with BisBAL NPs
2.3. Physicochemical Characterization of Tecoflex™ EMs-BisBAL NPs (SEM, FTIR, and Raman)
2.4. Water Absorption Capacity of Tecoflex™ EMs-BisBAL NPs
2.5. Tecoflex™ EM-BisBAL NPs Tensile Test
2.6. Biological Properties of Tecoflex™ EMs-BisBAL NPs
2.6.1. Cell Culture
2.6.2. Proliferation Assay
2.6.3. MRSA Isolation and Identification
2.6.4. Antimicrobial Activity against MRSA Clinical Isolate
2.6.5. Bacterial Culture
2.6.6. Bactericidal Activity of Tecoflex™ EMs-BisBAL NPs
2.6.7. Antitumor Activity of Tecoflex™ EMs-BisBAL NPs
2.6.8. Action Mechanism of Tecoflex™ EMs-BisBAL NPs
2.7. Statistical Analysis
3. Results
3.1. Characterization of BisBAL NPs
3.2. Scanning Electron Microscopy of Tecoflex™ EMs-BisBAL NPs
3.3. FTIR, Raman, Swelling and Tensile Strength Characterization of Tecoflex™ EMs-BisBAL NPs
3.4. Proliferation Assay
3.5. Identification and Resistance Analysis of a Bacterial Clinical Isolate
3.6. Bactericidal Activity of Tecoflex™ EMs-BisBAL NPs
3.7. Antitumor Potential of Tecoflex™ EMs-BisBAL NPs
3.8. Action Mechanism of Tecoflex™ EMs-BisBAL NPs
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Chemical Bond | Absorption Wavelength (cm−1) | |
---|---|---|
Drug-free Tecoflex™ | Tecoflex™ EM-BisBAL NPs | |
Free N-H | 3326 | 3449 and 3334 |
Asymmetric CH2 | 2932 and 2850 | Similar |
Symmetric CH2 | 2798 | Similar |
C=O (Amide I) | 1716 | Similar |
N-H and C-H (amide II) | 1525 | Similar |
CH2 bend | 1446–1366 | 1371 |
C-N bend (amide III) | 1228 | 1247 |
Polytetramethylene glycol C-O-C symmetric bend | 1100 | Similar |
Urethane C-O-C symmetric bend | 980 | Similar |
Tecoflex™ EMs-BisBAL NPs | 997, 956, 864, 836 | |
Cyclohexane symmetric bend | 779 | Similar |
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Torres-Betancourt, J.A.; Hernández-Delgadillo, R.; Cauich-Rodríguez, J.V.; Oliva-Rico, D.A.; Solis-Soto, J.M.; García-Cuellar, C.M.; Sánchez-Pérez, Y.; Pineda-Aguilar, N.; Flores-Treviño, S.; Meester, I.; et al. A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells. J. Funct. Biomater. 2024, 15, 309. https://doi.org/10.3390/jfb15100309
Torres-Betancourt JA, Hernández-Delgadillo R, Cauich-Rodríguez JV, Oliva-Rico DA, Solis-Soto JM, García-Cuellar CM, Sánchez-Pérez Y, Pineda-Aguilar N, Flores-Treviño S, Meester I, et al. A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells. Journal of Functional Biomaterials. 2024; 15(10):309. https://doi.org/10.3390/jfb15100309
Chicago/Turabian StyleTorres-Betancourt, Jesús Alejandro, Rene Hernández-Delgadillo, Juan Valerio Cauich-Rodríguez, Diego Adrián Oliva-Rico, Juan Manuel Solis-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Nayely Pineda-Aguilar, Samantha Flores-Treviño, Irene Meester, and et al. 2024. "A Polyurethane Electrospun Membrane Loaded with Bismuth Lipophilic Nanoparticles (BisBAL NPs): Proliferation, Bactericidal, and Antitumor Properties, and Effects on MRSA and Human Breast Cancer Cells" Journal of Functional Biomaterials 15, no. 10: 309. https://doi.org/10.3390/jfb15100309