Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction

Epanchintseva, Anna V.; Baranova, Svetlana V.; Poletaeva, Julia E.; Bakhno, Irina A.; Ryabchikova, Elena I.; Dovydenko, Ilya S.

doi:10.3390/nano14211767

Open AccessArticle

Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction

by

Anna V. Epanchintseva

^†,

Svetlana V. Baranova

^†

,

Julia E. Poletaeva

,

Irina A. Bakhno

,

Elena I. Ryabchikova

^*

and

Ilya S. Dovydenko

^*

Institute of Chemical Biology and Fundamental Medicine SB RAS, 630090 Novosibirsk, Russia

^*

Authors to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Nanomaterials 2024, 14(21), 1767; https://doi.org/10.3390/nano14211767

Submission received: 13 September 2024 / Revised: 12 October 2024 / Accepted: 14 October 2024 / Published: 4 November 2024

(This article belongs to the Special Issue Nanostructured Materials for Biological and Pharmaceutical Applications (Second Edition))

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Abstract

The composition of the protein corona covering any nanoparticle (NP) when it enters a biological fluid determines the parameters of the NP’s interaction with the body. To “control” these parameters, it is important to know the composition of the protein corona, the determination of which is a complex task associated with the two-layer organization of the corona (hard and soft coronas). In a previous publication, we reported obtaining lipid-coated NPs with a full protein corona, isolating them, and proving the presence of the corona on the surface of the NPs. This work reports on the preparation, isolation, and purification of lipid-coated NPs bearing a hard corona. The protein corona composition was determined by using the LC–MS/MS method. Thirty-seven serum proteins were identified with a high degree of reliability. The hard corona contained various apolipoproteins, including apolipoprotein E, which can potentially affect the penetration of NPs into the cell.

Keywords: multilevel nanoconstructs; gold nanoparticles; hard protein corona; LC–MS/MS analysis; apolipoproteins

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MDPI and ACS Style

Epanchintseva, A.V.; Baranova, S.V.; Poletaeva, J.E.; Bakhno, I.A.; Ryabchikova, E.I.; Dovydenko, I.S. Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction. Nanomaterials 2024, 14, 1767. https://doi.org/10.3390/nano14211767

AMA Style

Epanchintseva AV, Baranova SV, Poletaeva JE, Bakhno IA, Ryabchikova EI, Dovydenko IS. Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction. Nanomaterials. 2024; 14(21):1767. https://doi.org/10.3390/nano14211767

Chicago/Turabian Style

Epanchintseva, Anna V., Svetlana V. Baranova, Julia E. Poletaeva, Irina A. Bakhno, Elena I. Ryabchikova, and Ilya S. Dovydenko. 2024. "Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction" Nanomaterials 14, no. 21: 1767. https://doi.org/10.3390/nano14211767

APA Style

Epanchintseva, A. V., Baranova, S. V., Poletaeva, J. E., Bakhno, I. A., Ryabchikova, E. I., & Dovydenko, I. S. (2024). Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction. Nanomaterials, 14(21), 1767. https://doi.org/10.3390/nano14211767

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Study of Hard Protein Corona on Lipid Surface of Composite Nanoconstruction

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