Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability
Abstract
:1. Introduction
2. Various Types of Electrochemical Biosensors for Live-Cell Detection
2.1. ECIS
2.2. FET-Based Electrochemical Biosensors
2.3. Potentiometric-Based Electrochemical Biosensors
3. Application for Live-Cell Monitoring Based on Electrochemical Biosensing
3.1. Cancer Cell-Based Sensing and Assessment of Anticancer Drug Candidates
3.2. Stem Cell-Based Sensing and Monitoring for Differentiation
4. Conclusions
5. Future Perspectives
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Detection Method | Cell Line | Immobilisation Strategy | LOD (Target) | Ref |
---|---|---|---|---|
ECIS | SH-SY5Y, ND7/23 cells | PPy/s-MWCNTs/ AuNPs hydrogel/ITO | 17 nM (Dopamine) | [78] |
ECIS | MCF-7 | Tb-MOF-on-Fe-MOF | 58 μU/mL (CA125) | [79] |
ECIS | HeLa | FA@UiO-66 nanocomposite/Au | 90 cells/mL | [80] |
ECIS | HeLa, MDCK, 293T | CdZnSeS QD/OMC | 10.23 μM (H2O2) | [81] |
ECIS | HeLa | Cu2O–CuO@GQDs | 1 nM (Bisphenol A) | [82] |
ECIS | LNCaP | BPene@PDA−SCX8 | 36 cells/mL | [83] |
FET | PC12 | PVC coated CNT-FET | 1 nM (Potassium ion) | [84] |
FET | U-251 MG | Graphene-based ISFET | 1 μM (Potassium ion) | [85] |
FET | HepG2 | graphene foam FET | 0.5 pM (ATP) | [86] |
FET | HeLa | MoS2/RGO FET | 1 pM (H2O2) | [87] |
Amperometric | MCF-7 | ZnMn2O4/rGO | 0.012 μM (H2O2) | [88] |
DPV | MCF-7 | ITO/MWCNT/PDDAHA | 5.94 pg/mL (CD44) | [89] |
LSV | HepG2 | GC/ZNBs/fMWCNT | 35 nM (H2O2) | [90] |
Amperometric | Patient-derived cancer | MnO2-NWs@Au-NPs/GF | 1.9 μM (H2O2) | [91] |
Detection Method | Cell Lines | Immobilisation Strategy | Anticancer Drug | Ref |
---|---|---|---|---|
EIS | Cervical cancer (HeLa) | c-MWCNTs/AuNPs | Pinoresinol | [59] |
Amperometric | Lung cancer (H1299) | HRP-AuNPs-MWNT | fMLP | [107] |
CV | Liver cancer (HepG2) | G-quadruplex/hemin/Au /QZIF-67-2/GCE | Paclitaxel | [108] |
SECM | Patient-derived cancer organoid (colorectal) | Fibrin-collagen gel | Bortezomib | [109] |
SWV | Cervical cancer (HeLa) | Telomerase and dNTPs | Epigallocatechin gallate (EGCG) | [110] |
Amperometric | Lung cancer (PC9) | PHF-MWNT-PB-Gox | Osimertini | [111] |
EIS | Cervical cancer (HeLa) | C-Kemptide-modified AuNP /rGO-GCE | H-89 | [112] |
Detection Method | Cell Lines | Differentiated Cells | LOD (Target) | Ref |
---|---|---|---|---|
DPV | Human embryonic stem cell | Undifferentiated stem cell | 12,500 cells (ESC) | [57] |
SWV | Human tissue | Cancer stem cell | 0.5–1000 ng/mL (Oct4) | [113] |
CV | Human mesenchymal stem cell | Osteogenic differentiation | 0.03 unit/mL (ALP) | [114] |
Amperometric | Human embryonic stem cell | Brain organoid | 0.5 mM (glutamine) | [115] |
EIS | Human neuroepithelial stem cell | Midbrain organoid | 8 nM (epinephrine) | [116] |
DPV | Mouse neural stem cell | Neuronal differentiation | 134 nM (dopamine) | [117] |
DPV | Bone mesenchymal stem cell | Cardiomyogenic differentiation | 0.42 pg/mL (cTnI) | [118] |
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Koo, K.-M.; Kim, C.-D.; Ju, F.N.; Kim, H.; Kim, C.-H.; Kim, T.-H. Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability. Biosensors 2022, 12, 1162. https://doi.org/10.3390/bios12121162
Koo K-M, Kim C-D, Ju FN, Kim H, Kim C-H, Kim T-H. Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability. Biosensors. 2022; 12(12):1162. https://doi.org/10.3390/bios12121162
Chicago/Turabian StyleKoo, Kyeong-Mo, Chang-Dae Kim, Fu Nan Ju, Huijung Kim, Cheol-Hwi Kim, and Tae-Hyung Kim. 2022. "Recent Advances in Electrochemical Biosensors for Monitoring Animal Cell Function and Viability" Biosensors 12, no. 12: 1162. https://doi.org/10.3390/bios12121162