A Microfluidic Platform with an Embedded Miniaturized Electrochemical Sensor for On-Chip Plasma Extraction Followed by In Situ High-Sensitivity C-Reactive Protein (hs-CRP) Detection
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagent and Sample Preparation
2.2. Microchannel Fabrication and Assembly
2.3. Instrument Setup
3. Results and Discussion
3.1. Operation Protocol of the Microfluidic Platform
3.2. Plasma Extraction Efficiency
3.3. The Electrochemical (EC) Sensor Functionalization Protocol
3.4. Specificity Test
3.5. CRP Detection
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Conflicts of Interest
References
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Lai, Z.-X.; Wu, C.-C.; Huang, N.-T. A Microfluidic Platform with an Embedded Miniaturized Electrochemical Sensor for On-Chip Plasma Extraction Followed by In Situ High-Sensitivity C-Reactive Protein (hs-CRP) Detection. Biosensors 2022, 12, 1163. https://doi.org/10.3390/bios12121163
Lai Z-X, Wu C-C, Huang N-T. A Microfluidic Platform with an Embedded Miniaturized Electrochemical Sensor for On-Chip Plasma Extraction Followed by In Situ High-Sensitivity C-Reactive Protein (hs-CRP) Detection. Biosensors. 2022; 12(12):1163. https://doi.org/10.3390/bios12121163
Chicago/Turabian StyleLai, Zhi-Xuan, Chia-Chien Wu, and Nien-Tsu Huang. 2022. "A Microfluidic Platform with an Embedded Miniaturized Electrochemical Sensor for On-Chip Plasma Extraction Followed by In Situ High-Sensitivity C-Reactive Protein (hs-CRP) Detection" Biosensors 12, no. 12: 1163. https://doi.org/10.3390/bios12121163