Cross-Contamination versus Outbreak: Pre-XDR Mycobacterial Strains Confirmed by Whole-Genome Sequencing
Abstract
:1. Introduction
2. Results
2.1. Whole-Genome Sequencing Data
2.2. Correlation with Epidemiological and Clinical Information
3. Discussion
4. Materials and Methods
4.1. Correlation with Epidemiological and Clinical Information
4.2. Whole-Genome Sequencing
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Case No. | Smear Date | Smear Results * | Culture Date | Culture Results | Concordance of Strain | H | RFP | SM | E | Km | Cm | PTH | CS | PAS | Ofx | Mfx | Amk | Lfx | Rib | Z | LNZ |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
No. 1 | 21 May | − | 21 May | 1+ | Concordant 1 | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S |
23 May | 4+ | 23 May | 4+ | ||||||||||||||||||
24 May | 4+ | 24 May | 1+ | ||||||||||||||||||
25 May | 4+ | 25 May | 1+ | ||||||||||||||||||
26 May | 4+ | 26 May | 1+ | ||||||||||||||||||
1st June | 2+ | 1 June | 1+ | ||||||||||||||||||
No. 1 WGS | R | R | S | S | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 2 | 24 May | − | 25 May | 1+ | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |
No. 2. WGS | R | R | S | S | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 3 | 27 May | − | 27 May | 2+ | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |
No. 3 WGS | R | R | S | S | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 4 | 24 May | − | 24 May | 1+ | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |
No. 4 WGS | R | R | S | S | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 5 | 27 May | − | 27 May | 2+ | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |
No. 5 WGS | R | R | S | S | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 6-1 (sputum) | 2 June | + | 26 June | 1+ | Concordant 2 | R | R | R | R | S | S | S | S | S | S | S | S | S | R | R | S |
No. 6-1 WGS | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |||||
No. 6-2 (bfs) | 4 July | 2+ | 4 July | 1+ | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S | |
No. 6-2 WGS | R | R | R | R | S | S | S | S | S | R | R | S | R | R | R | S |
Case No. | Age/Sex | Underlying Disease | Symptoms | Past TB History | Chest CT Findings | Bronchoscopy | TB Treatment | Clinical Diagnosis | Laboratory Investigation | |
---|---|---|---|---|---|---|---|---|---|---|
Previous Hospital | After Referral | |||||||||
1 | 58/Male | Chronic alcoholics | Fever, anorexia | − | Multiple cavities and consolidation in both lungs | Not done | Yes | Expired | Active TB | True strain |
2 | 69/Male | DM, HTN, Cerebral infarction | Cough, sputum | + | Multiple centrilobular GGOs and nodules in RML/RLL | No endobronchial lesion | Yes | No | CAP | Contamination |
3 | 73/Female | HTN, Spinal stenosis | Cough, sputum | − | Diffuse ill-defined centrilobular nodules and GGO in both lungs | Anthracofibrosis | Yes | No | Bronchitis | Contamination |
4 | 71/Male | HTN, Asthma, Unstable angina, CKD, Panic disorder | General weakness | + | Multiple calcified granulomas, irregular pleural thickening, and bronchiectasis | Anthracofibrosis | Yes | Yes | Bronchitis | Contamination |
5 | 32/Female | none | Cough, sputum | + | Consolidation, calcified granulomas, fibrosis, and pleural thickening in both lungs | Anthracotic pigmentation | Yes | No | CAP | Contamination |
6 | 55/Female | HTN | Cough, sputum | − | Multiple ill-defined centrilobular nodules and patchy consolidations in LUL | Endobronchial TB (left main) | Yes | Yes | Active TB | True strain |
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Oh, J.Y.; Park, K.H.; Lee, J.; Kim, D.; Seok, K.H.; Oh, I.-H.; Lee, S.H. Cross-Contamination versus Outbreak: Pre-XDR Mycobacterial Strains Confirmed by Whole-Genome Sequencing. Antibiotics 2021, 10, 297. https://doi.org/10.3390/antibiotics10030297
Oh JY, Park KH, Lee J, Kim D, Seok KH, Oh I-H, Lee SH. Cross-Contamination versus Outbreak: Pre-XDR Mycobacterial Strains Confirmed by Whole-Genome Sequencing. Antibiotics. 2021; 10(3):297. https://doi.org/10.3390/antibiotics10030297
Chicago/Turabian StyleOh, Jee Youn, Kyung Ho Park, Jisoon Lee, Donghyeok Kim, Kwang Hyuk Seok, In-Hwan Oh, and Seung Heon Lee. 2021. "Cross-Contamination versus Outbreak: Pre-XDR Mycobacterial Strains Confirmed by Whole-Genome Sequencing" Antibiotics 10, no. 3: 297. https://doi.org/10.3390/antibiotics10030297
APA StyleOh, J. Y., Park, K. H., Lee, J., Kim, D., Seok, K. H., Oh, I. -H., & Lee, S. H. (2021). Cross-Contamination versus Outbreak: Pre-XDR Mycobacterial Strains Confirmed by Whole-Genome Sequencing. Antibiotics, 10(3), 297. https://doi.org/10.3390/antibiotics10030297