Evaluation of β-Lactamase Enzyme Activity in Outer Membrane Vesicles (OMVs) Isolated from Extended Spectrum β-Lactamase (ESBL) Salmonella Infantis Strains
Abstract
:1. Introduction
2. Results
2.1. Transmission Electron Microscopy (TEM)
2.2. Dynamic Light Scattering (DLS) Analysis
2.3. Evaluation of β-Lactamase Enzymatic Activity
3. Discussion
4. Materials and Methods
4.1. Experimental Design
4.2. Isolation of S. Infantis OMVs
4.3. Transmission Electron Microscopy (TEM)
4.4. Dynamic Light Scattering (DLS)
4.5. Evaluation of β-Lactamase Activity
4.6. Protein Content Quantification
4.7. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Toppi, V.; Scattini, G.; Musa, L.; Stefanetti, V.; Pascucci, L.; Chiaradia, E.; Tognoloni, A.; Giovagnoli, S.; Franciosini, M.P.; Branciari, R.; et al. Evaluation of β-Lactamase Enzyme Activity in Outer Membrane Vesicles (OMVs) Isolated from Extended Spectrum β-Lactamase (ESBL) Salmonella Infantis Strains. Antibiotics 2023, 12, 744. https://doi.org/10.3390/antibiotics12040744
Toppi V, Scattini G, Musa L, Stefanetti V, Pascucci L, Chiaradia E, Tognoloni A, Giovagnoli S, Franciosini MP, Branciari R, et al. Evaluation of β-Lactamase Enzyme Activity in Outer Membrane Vesicles (OMVs) Isolated from Extended Spectrum β-Lactamase (ESBL) Salmonella Infantis Strains. Antibiotics. 2023; 12(4):744. https://doi.org/10.3390/antibiotics12040744
Chicago/Turabian StyleToppi, Valeria, Gabriele Scattini, Laura Musa, Valentina Stefanetti, Luisa Pascucci, Elisabetta Chiaradia, Alessia Tognoloni, Stefano Giovagnoli, Maria Pia Franciosini, Raffaella Branciari, and et al. 2023. "Evaluation of β-Lactamase Enzyme Activity in Outer Membrane Vesicles (OMVs) Isolated from Extended Spectrum β-Lactamase (ESBL) Salmonella Infantis Strains" Antibiotics 12, no. 4: 744. https://doi.org/10.3390/antibiotics12040744