Obtaining of Antibacterial Nanoporous Layer on Ti7.5Mo Alloy Surface Combining Alkaline Treatment and Silver: In Vitro Studies
, Willian Fernando Zambuzzi 3, Patricia Capellato 4, Daniela Sachs 4 and Ana Paula Rosifini Alves 1,2,*
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsManuscript ID: coatings-2683641
Despite the efforts done by the authors, and the manuscript contains a good story, there are some important points that should be considered by the authors.
1. The manuscript title should be modified to be more relevant to the aim and results of the study. Please use one format when writing the manuscript title.
2. In abstract "The methodology employed was based on previous studies conducted by the group, which enabled the acquisition of a nanostructured surface containing silver particles…..". What do you mean by group? Which group?
3. The abstract should be specific and brief. It must contain the objective of the work, a summary of the experiments, and the most important results.
4. Keywords must have words that express the purpose of the research. Please add more words.
5. In conclusion, please clarify the main finding of your experimental study.
6. In previous studies conducted by our group, we utilized two different types of surface treatment techniques….., Please, add references, for example: In previous studies [R1, R2].
7. The novelty of the current study should be highlighted at the end of the introduction
8. The manuscript needs linguistic review. For example "Samples were melted in an arc furnace an argon atmosphere"
9. Please, check Table 1. What is the meaning of "Condição de reação"
10. The quality of Figs.3, 5 and 6 should be improved.
11. Update the cited references.
Comments for author File: 
Comments.pdf
must be improved
Author Response
We appreciate the suggestions.
The revisions are attached.
Author Response File: Author Response.pdf
Reviewer 2 Report
Comments and Suggestions for AuthorsThe paper, entitled oCatings-2683641, is well written, clear and concise, the results are well presented, the discussion includes all the results shown, and the conclusion is consistent with the measurements shown.
I recommend the paper for publication with minor changes.
Page 2, line 85
I ask the authors to remove this technical error "to the treatment.which was divided into 3 stages:"
Page 13, line 363
Similar error, please correct
"proteins such as kinases. as focal adhesion kinase (FAK) and Src"
Page 2, line 88
80ºC, 60ºC... for some reason the degree is underlined (also in other places), this needs to be corrected.
Page 5, 6 at the bottom of the image titles, the color of the text is slightly different than black.
Page 9, Figures 5 and 6, it would be good if the authors could provide a better resolution of these images.
Page 11, Figure 7, the markings aren't clearly visible in the picture.
Check the references in detail, in terms of stating the year, volume and page... I ask the authors to look at how exactly the numbering of the papers should be stated.
Author Response
We appreciate the suggestions and forward them as attachments.
Author Response File: Author Response.pdf
Reviewer 3 Report
Comments and Suggestions for AuthorsAfter analyzing the full paper, it is found that there are many similar published results, author needs to explain the novelty of the present work in the Introduction part. In addition, there are many shortcomings that need to be revised, details are as follows:
1. The font color of the caption in Figure 1 is not consistent, and a ruler needs to be given;
2. Figs. 2 and 3 are not very clear, the resolution should be improved and the ruler needs to be given;
3. The measured contact Angle should be drawn in Fig. 4;
4. The clarity of Figs. 5 and 6 need to be improved;
5. The ruler in Fig. 7 cannot be seen clearly;
6. The format of the references should be consistent;
7. Some new references need to be added, such as:
[1] Rare Metals, 2023, 42(9): 3114–3129.
[2] Rare Metal Materials and Engineering, 2021, 50(11): 4165-4173.
Author Response
We appreciate the suggestions and forward them as attachments.
Author Response File: Author Response.pdf
Reviewer 4 Report
Comments and Suggestions for AuthorsGood piece of work. Major revisions in order.
“ve in this research was to alter the surface of the Ti 7.5Mo alloy by utilizing”: why this specific alloy?
“showed that there is no difference in cytotoxicity after silver immobilization”: was this expected? Why?
“er showed a better response in terms of colony formation, meaning that less bacterial adhesion was detected”: how is this quantified?
“Titanium and its alloys play an important role due to their bulk properties, such as high mechanical resistance, low elastic modulus, and fatigue strength, which are associated with their excellent biocompatibility”: as detailed elsewhere. See for example 10.1016/j.matchar.2020.110400, 10.1016/j.matchar.2020.110180 and 10.1038/s41597-022-01283-9 and complement.
“mplants is the occurrence of local infection”: of what type?
“t leads to a bioactive nanostructured surface[15, 16, 17], while anodic oxidation produce”: what is the significance? Detail please.
“The Ti–7.5Mo alloy was produced”: at. or wt?
What is the alloy phase structure? Was this checked? Does it impact the colonization of cells?
Fig 2 needs a scale.
Fig 5 is too pixelated. Cannot be properly seen.
“hanisms can be regulated by extracellular matrix remodeling, which is regulated by the activity of metalloproteinases”: in what way? Needs to be discussed.
“hat surface topographic promotion of osseointegration depends on the ability to induc”: is there any limitation on the topographic dimensions of the material? Detail please.
“Cell interactions with the extracellular matrix play a key role in the cell adhesion process.”: in what way? Unclear.
“nhibiting various cell functions and causing cell death has been described by several authors”: where? Must be shown.
Comments on the Quality of English Language
good
Author Response
We appreciate the suggestions and forward them as attachments.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report
Comments and Suggestions for AuthorsAll the modifications have been achieved.
Reviewer 3 Report
Comments and Suggestions for AuthorsThe paper can be published
Reviewer 4 Report
Comments and Suggestions for AuthorsAcceptance is recommended
