The Tardigrade Damage Suppressor Protein Modulates Transcription Factor and DNA Repair Genes in Human Cells Treated with Hydroxyl Radicals and UV-C
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Transfection
2.2. Evaluation of Dsup Transcript Presence by Endpoint and Real-Time Reverse Transcriptase-PCR
2.3. Cell Viability
2.4. Cyclobutane Pyrimidine Dimers (CPDs) Evaluation
2.5. Transcription Factor Evaluation
2.6. Gene Expression Analysis
2.7. Statistical Analysis
3. Results
3.1. Cell Survival under Stress Conditions
3.2. Transcription Factor Activation in Response to Stress Condition
3.3. Gene Expression in Response to Stress Conditions
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ricci, C.; Riolo, G.; Marzocchi, C.; Brunetti, J.; Pini, A.; Cantara, S. The Tardigrade Damage Suppressor Protein Modulates Transcription Factor and DNA Repair Genes in Human Cells Treated with Hydroxyl Radicals and UV-C. Biology 2021, 10, 970. https://doi.org/10.3390/biology10100970
Ricci C, Riolo G, Marzocchi C, Brunetti J, Pini A, Cantara S. The Tardigrade Damage Suppressor Protein Modulates Transcription Factor and DNA Repair Genes in Human Cells Treated with Hydroxyl Radicals and UV-C. Biology. 2021; 10(10):970. https://doi.org/10.3390/biology10100970
Chicago/Turabian StyleRicci, Claudia, Giulia Riolo, Carlotta Marzocchi, Jlenia Brunetti, Alessandro Pini, and Silvia Cantara. 2021. "The Tardigrade Damage Suppressor Protein Modulates Transcription Factor and DNA Repair Genes in Human Cells Treated with Hydroxyl Radicals and UV-C" Biology 10, no. 10: 970. https://doi.org/10.3390/biology10100970
APA StyleRicci, C., Riolo, G., Marzocchi, C., Brunetti, J., Pini, A., & Cantara, S. (2021). The Tardigrade Damage Suppressor Protein Modulates Transcription Factor and DNA Repair Genes in Human Cells Treated with Hydroxyl Radicals and UV-C. Biology, 10(10), 970. https://doi.org/10.3390/biology10100970