Expression of Semaphorin 3A in Malignant and Normal Bladder Tissue: Immunohistochemistry Staining and Morphometric Evaluation
, Sarel Halachmi 5 and Zahava Vadasz 6Round 1
Reviewer 1 Report
This paper looks at protein expression (Sema3A) in UCC versus normal tissue and detects a tangible difference between the two. Moreover, there is a difference in pattern of expression from LG to HG and CIS. This is an interesting finding. I would have someone fluent in English read the paper to fix the minor mistakes spotted in the paper. Beside that, use the newer terminology (UCC instead of TCC). Figure 2 needs to be color coded so the reader can understand what each column presents. Otherwise, this is a nicely done paper.Author Response
Point 1: I would have someone fluent in English read the paper to fix the minor mistakes spotted in the paper
Answer 1: revisions were made to fix minor language flaws.
Point 2: use the newer terminology (UCC instead of TCC)
Answer 2: thank you so much for your comment, this was corrected
Point 3: Figure 2 needs to be color coded so the reader can understand what each column presents
Answer 3: The figure is now color-coded
Reviewer 2 Report
1) Well written article. Conclusions are supported by the evidence provided.
2) Please expand BCG in the introduction.
3) It would be helpful for the readers if FIG 2 image is annotated with demarcations for the three layers: basal, intermediate and apical.
Author Response
Point 1: Well written article. Conclusions are supported by the evidence provided.
Answer: thank you so much
Point 2: Please expand BCG in the introduction.
Answer: thank you for the comment. done!
Point 3: It would be helpful for the readers if FIG 2 image is annotated with demarcations for the three layers: basal, intermediate and apical.
Answer: excellent point. Done!
Reviewer 3 Report
The manuscript is written correctly, the experiments are adequate and the results are discussed. However, both the introduction and the discussion lack recent references on the role of Semaphorin 3A. In particular, the controversial Semaphorin role should be discussed, perhaps discussing recent publications e.g. such as the following references:
Pham HT, Kondo S, Endo K, Wakisaka N, Aoki Y, Nakanishi Y, Kase K, Mizokami
H, Kano M, Ueno T, Hatano M, Moriyama-Kita M, Sugimoto H, Yoshizaki T.
Influences of Semaphorin 3A Expression on Clinicopathological Features, Human Papillomavirus Status, and Prognosis in Oropharyngeal Carcinoma. Microorganisms. 2020 Aug 22;8(9):1286.
Izycka N, Sterzynska K, Januchowski R, Nowak-Markwitz E. Semaphorin 3A
(SEMA3A), protocadherin 9 (PCdh9), and S100 calcium binding protein A3 (S100A3)as potential biomarkers of carcinogenesis and chemoresistance of differentneoplasms, including ovarian cancer - review of literature. Ginekol Pol.
2019;90(4):223-227.
Author Response
Point 1: The manuscript is written correctly, the experiments are adequate and the results are discussed. However, both the introduction and the discussion lack recent references on the role of Semaphorin 3A. In particular, the controversial Semaphorin role should be discussed, perhaps discussing recent publications e.g. such as the following references:
Answer: We appreciate this comment. we have updated with relevant recent publications
