Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Mice and Their Diets
2.2. Histology
2.3. RNA Separation and cDNA Synthesis
2.4. Reverse Transcription PCR (RT-PCR)
2.5. Quantitative Real-Time PCR (qRT-PCR)
2.6. Mass Spectrometric Measurement of Serum Vitamin D
2.7. Measurement of Gastric Acid
2.8. Statistical Analysis
3. Results
3.1. Expression of the VDR in Normal Gastric Epithelial Tissues
3.2. The VDR Is Expressed by Gastric Parietal and Mucous Cells
3.3. Gastric Expression of CYP Enzymes Involved in Regulating VD3
3.4. Establishment of a VDD Mouse Model
3.5. Low Gastric Acid Content along with Increased Gastrin Gene Expression in the VDD Stomach
3.6. A Noticeable Increase in Gastric Stem/Progenitor Cell Proliferation in the VDD Group of Mice
3.7. Gastric Differentiation Markers and Vdr Expression Are Affected by Vitamin D Status
3.8. The Expression of the Target Genes of 1α,25(OH)2D3 in Response to Vitamin D Status
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ali, I.I.; Shah, I.; Marzouk, S.; Karam, S.M.; Al Menhali, A. Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis. Biology 2021, 10, 705. https://doi.org/10.3390/biology10080705
Ali II, Shah I, Marzouk S, Karam SM, Al Menhali A. Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis. Biology. 2021; 10(8):705. https://doi.org/10.3390/biology10080705
Chicago/Turabian StyleAli, Ifrah Ismail, Iltaf Shah, Sayed Marzouk, Sherif M. Karam, and Asma Al Menhali. 2021. "Vitamin D Is Necessary for Murine Gastric Epithelial Homeostasis" Biology 10, no. 8: 705. https://doi.org/10.3390/biology10080705