Isolation and Functional Characterization of a Green-Tissue Promoter in Japonica Rice (Oryza sativa subsp. Japonica)
, Shaojuan Wang 2, Shengnan Tian 2, Faxing Chen 2,* and Zhimin Lin 1,*
Round 1
Reviewer 1 Report
This manuscript is well written, but I only observe that most of the figures the description is not clear, for example figure 7 a the horizontal and vertical axis is not clear. this needs to improve.
Author Response
This manuscript is well written, but I only observe that most of the figures the description is not clear, for example figure 7 a the horizontal and vertical axis is not clear. this needs to improve.
Response: Respected reviewer, thank you so much for the appreciation of our work. The figures are replaced with more clear ones. The figure 7a has also been improved.
Reviewer 2 Report
1. Since the promoter can drive GUS expression in root, you can't say "Green-Tissue Promoter OsGSX7". You may change to "Green-Tissue preferred..".
2. Explain what is reverse promoter in the introduction section more clearly.
3. Line 150-151: "The reverse promoter GSX7R-2097 derived the expression of exogenous GUS gene in the green tissue of rice, including root, stem, leaf, glume, flower and embryo, but not in endosperm". I don't think root is green tissue, you should modify it and similar sentences across the manuscript.
4. Fig 4: No. 2 lane has multiple bands, you should indicate which band is the target band.
5. Line 229-230: "Figure 8bB,bD" change to "Figure 8b B&D"; "Figure 8bA,bC" change to " Figure 8b A&C".
6. Line 260: what is the difference of the two "G-Box, G-box"?
7. Line 283: "genes by interacting with transcription factors such as CCAAT box and MYB". I don't think CCAAT box is a transcription factor. The authors should read the manuscript carefully again and improve the English writing.
Author Response
- Since the promoter can drive GUS expression in root, you can't say "Green-Tissue Promoter OsGSX7". You may change to "Green-Tissue preferred..".
Response: Thank you for the valuable suggestion. The title has been modified.
- Explain what is reverse promoter in the introduction section more clearly.
Response: The introduction of reverse promoter has been incorporated in Introduction section (see lines 64-67)
- Line 150-151: "The reverse promoter GSX7R-2097 derived the expression of exogenous GUS gene in the green tissue of rice, including root, stem, leaf, glume, flower and embryo, but not in endosperm". I don't think root is green tissue, you should modify it and similar sentences across the manuscript.
Response: Thank you for the valuable suggestion. The sentence has been modified and pointed change has been incorporated in whole manuscript.
- Fig 4: No. 2 lane has multiple bands, you should indicate which band is the target band.
Response: The amplified bands are indicated by red arrows now.
- Line 229-230: "Figure 8bB,bD" change to "Figure 8b B&D"; "Figure 8bA,bC" change to " Figure 8b A&C".
Response: The figure citations style has been modified, as suggested.
- Line 260: what is the difference of the two "G-Box, G-box"?
Response: The G-Box and G-box are two different cis elements having different sequences.
- Line 283: "genes by interacting with transcription factors such as CCAAT box and MYB". I don't think CCAAT box is a transcription factor. The authors should read the manuscript carefully again and improve the English writing.
Response: Thanks for the correction. The text has been modified (see lines 287-288).
Reviewer 3 Report
Very good job! Well done
Author Response
Thank you so much for appreciation
