The Morphological and Molecular Characterization of the Avian Trematodes Harrahium obscurum and Morishitium dollfusi (Digenea: Cyclocoelidae) from the Middle Volga Region (European Russia)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Trematodes’ Collection and Morphological Examination
2.2. Molecular Data and Phylogenetic Analysis
3. Results
3.1. Systematic and Morphological Characteristics of the Studied Cyclocoelids
3.2. Molecular Phylogenetic Analysis
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Family | Species | Host | Locality | GenBank Number | References | |
---|---|---|---|---|---|---|
28S rDNA | cox1 mtDNA | |||||
Cyclocoelidae | Anativermis normdroneni | Branta canadensis (Anatidae) | USA | OQ780427 | – | [30] |
Anativermis normdroneni | Branta canadensis (Anatidae) | USA | OQ780428 | – | [30] | |
Circumvitellatrema momota | Momotus momota (Momotidae) | France (captive-born in zoo) | JQ886067 | – | [4] | |
Circumvitellatrema momota | Momotus momota (Momotidae) | France (captive-born in zoo) | JQ886066 | – | [4] | |
Cyclocoelum mutabile | Calidris canutus (Scolopacidae), Gallinula chloropus (Rallidae) | Peru | AY222249 | MH091807 | [31,32] | |
Harrahium obscurum | Tringa ochropus (Scolopacidae) | Russia | PP935228 | PP934665 | This study | |
Harrahium obscurum | Tringa nebularia, Tringa erythropus (Scolopacidae) | Czech Republic | – | KU877888 KU877884 KU877890 | [1] | |
Harrahium tringae | Tringa erythropus (Scolopacidae) | Czech Republic | – | KU877889 | [1] | |
Morishitium dollfusi | Pica pica (Corvidae), Turdus merula (Turdidae) | Czech Republic, Italy | OR364989 OR364990 OR364991 | OR364777 OR364782 MH800193 KU877887 KU877885 | [1,6,7] | |
Morishitium dollfusi | Turdus merula (Turdidae) | Russia | PP935227 | PP934664 | This study | |
Morishitium polonicum | Aplonis panayensis strigata (Sturnidae) | Malaysia | LC520231 | – | [33] | |
Morishitium polonicum malayense | Aplonis panayensis strigata (Sturnidae) | Malaysia | – | LC517300 LC517302 LC517301 | [33] | |
Neohaematotrephus arayae | Jacana spinosa (Jacanidae) | Mexico | MH725787 | – | [34] | |
Tracheophilus cymbius | Cairina moschata (Anatidae) | China | MK327367 | – | Not published | |
Typhlocoelum sp. | Anas platyrhynchos (Anatidae) | USA | KT956960 | – | [5] | |
Typhlocoelum cucumerinum | Cairina moschata (Anatidae) | Brazil | – | MZ254997 | [35] | |
Echinostomatidae | Himasthla elongata | Littorina littorea (Littorinidae) | Russia | MT987600 | – | [36] |
This Study | Harrah [37], Dubois [38] on Original Material | Sitko et al. [1] | Lamothe-Argumedo, Orozco-Flores [39] | |
---|---|---|---|---|
Host | Tringa ochropus | ? | Tringa erythropus | Tringa semipalmata |
Locality | Mordovia NR | USA | Czech Republic | Mexico |
Body length | 10.00–15.00 (11.96) | 6.0–13.00 | 13.00–28.00 (20.71) | 18.90–21.70 |
Body width | 1.750–3.100 (2.392) | 1.50–3.00 | 3.00–6.50 (4.80) | 3.50–4.30 |
Pharynx width | 0.183–0.362 (0.258) | 0.115–0.264 | 0.183–0.350 (0.254) | 0.270–0.372 |
Esophagus length | 0.308–0.462 (0.374) | 0.500–0.750 | – | 0.888 2 |
Cirrus sac length | 0.385–0.539 (0.442) | 0.248–0.579 | – | 0.777 2 |
Anterior testis length | 0.323–0.492 (0.422) | 0.462–0.827 | 0.486–1.143 (0.828) | 1.110–2.180 |
Anterior testis width | 0.400–0.539 (0.476) | 0.380–0.827 | 0.457–1.222 (0.923) | 1.440–2.500 |
Posterior testis length | 0.354–0.585 (0.424) | 0.480–1.000 | 0.514–1.457 (0.940) | 1.010–1.819 |
Posterior testis width | 0.385–0.662 (0.501) | 0.300–0.877 | 0.514–1.341 (0.986) | 1.300–1.980 |
Ovary length | 0.262–0.339 (0.300) | 0.275–0.463 1 | 0.343–0.571 (0.482) | 0.410–0.480 |
Ovary width | 0.246–0.354 (0.286) | 0.343–0.596 (0.483) | 0.430–0.560 | |
Intertesticular space length | 0.658–1.785 (1.122) | 0.830 | – | 1.888 2 |
Post-testicular space length | 0.400–0.754 (0.565) | 0.415 | – | 0.444 2 |
Egg length | 0.109–0.139 (0.127) | 0.138–0.162 | 0.133–0.157 (0.142) | 0.144–0.161 |
Egg width | 0.044–0.077 (0.069) | 0.070–0.094 | 0.070–0.087 (0.082) | 0.090 |
This Study | Timon-David [40] | Kirillova et al. [10] | Machalska [19] | Heneberg, Sitko [7] | Jaume-Ramis, Pinya [20] | |
---|---|---|---|---|---|---|
Host | Turdus merula | Pica pica | Turdus merula, Coccothraustes coccothraustes | Turdus philomelos | Turdus merula, Turdus philomelos | Turdus philomelos |
Locality | Zhiguli NR (Russia) | France | Smolny NP (Russia) | Poland | Czech Republic | Spain |
Body length | 8.00–11.75 (9.78) | 15.50–20.00 (17.00) | 6.250–8.254 | 7.138–13.109 | 6.850–21.500 (10.580) | 12.00 |
Body width | 1.90–2.75 (2.34) | – | 1.815–2.650 | – | 2.000–4.100 (2.513) | 2.50 |
Oral sucker width | 0.169–0.285 (0.219) | 0.350 | 0.169–0.266 | 0.208–0.323 | 184–368 (264) × 184–322 (246) 2 | 0.230 |
Pharynx width | 0.221–0.277 (0.251) | 0.250 | 0.193–0.266 | 0.208–0.323 | 193–285 (246) × 202–359 (261) 2 | 0.235 |
Esophagus length | 0.185–0.339 (0.248) | 0.250–0.425 | – | – | 0.230–0.644 (0.453) | – |
Cirrus sac length | 0.231–0.400 (0.299) | 0.255 | 0.193–0.324 | 0.219–0.474 | 0.203–0.644 (0.368) × 116–0.230 (0.166) 2 | 0.365 |
Anterior testis length | 0.385–0.769 (0.619) | 0.910 | 0.370–1.000 | 0.498 1 | 0.402–0.984 (0.687) | 0.715 |
Anterior testis width | 0.462–0.739 (0.589) | 1.350 | 0.373–0.984 (0.592) | – | ||
Posterior testis length | 0.492–0.862 (0.679) | 0.910 | 0.373–1.222 (0.820) | – | ||
Posterior testis width | 0.554–0.923 (0.695) | 1.350 | 0.447–1.043 (0.701) | – | ||
Ovary length | 0.200–0.308 (0.263) | 0.320–0.400 | 0.222–0.348 | 0.265 1 | 0.184–0.507 (0.307) | 0.280 |
Ovary width | 0.262–0.354 (0.301) | 0.230–0.745 (0.311) | – | |||
Intertesticular space length | 0.446–1.092 (0.759) | 1.920 | 0.533–0.963 | 1.430 | 0.343–1.143 (0.773) | – |
Post-testicular space length | 0.677–1.062 (0.842) | 1.590 | 0.444–0.954 | 1.260 | 0.571–1.714 (0.946) | – |
Egg length | 0.123–0.154 (0.135) | 0.120–0.130 | 0.079–0.134 | 0.081–0.139 | 0.128–0.139 (0.137) | 0.120 |
Egg width | 0.064–0.079 (0.070) | 0.043–0.071 | 0.046–0.069 | 0.070–0.081 (0.078) | 0.060 |
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Kirillov, A.A.; Kirillova, N.Y.; Shchenkov, S.V.; Knyazev, A.E.; Vekhnik, V.A. The Morphological and Molecular Characterization of the Avian Trematodes Harrahium obscurum and Morishitium dollfusi (Digenea: Cyclocoelidae) from the Middle Volga Region (European Russia). Biology 2024, 13, 621. https://doi.org/10.3390/biology13080621
Kirillov AA, Kirillova NY, Shchenkov SV, Knyazev AE, Vekhnik VA. The Morphological and Molecular Characterization of the Avian Trematodes Harrahium obscurum and Morishitium dollfusi (Digenea: Cyclocoelidae) from the Middle Volga Region (European Russia). Biology. 2024; 13(8):621. https://doi.org/10.3390/biology13080621
Chicago/Turabian StyleKirillov, Alexander A., Nadezhda Yu. Kirillova, Sergei V. Shchenkov, Alexei E. Knyazev, and Victoria A. Vekhnik. 2024. "The Morphological and Molecular Characterization of the Avian Trematodes Harrahium obscurum and Morishitium dollfusi (Digenea: Cyclocoelidae) from the Middle Volga Region (European Russia)" Biology 13, no. 8: 621. https://doi.org/10.3390/biology13080621