Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Experimental Animals
2.2. In Vitro Treatments
2.3. In Vivo Treatments
2.4. Determination of Ecdysteroid Levels
2.5. Gene Expression Analysis
2.6. Statistical Analysis
3. Results
3.1. Expression Profiles of PtCrz and PtCrzR during the Molt Cycle
3.2. Effects of PtCrz Peptide and dsPtCrzR on Ecdysteroid Synthesis In Vitro
3.3. Effects of PtCrz Peptide and dsPtCrzR on Ecdysteroid Synthesis In Vivo
3.4. Effects of PtCrz/PtCrzR Signaling on PtETH Expression
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Primer (5′-3′) | GenBank Accession No. |
---|---|---|
PtCrz | Forward: CAGTTGTGGTGCTCGTTGCC | OL694705 |
Reverse: GCTCAGCGGACCTTTTTCG | ||
PtCrzR | Forward: GTCATCTGCTGGACTCCCTACTAC | OL694706 |
Reverse: CGGGTTGACGAGACTGTTGG | ||
PtSpo | Forward: GTTTTGGCTCCCGCAACTA | KM030021 |
Reverse: TGTCGTCGGTGAGGCTTGT | ||
PtSad | Forward: CAGATATGGGCAGATTCATCG | KM596851 |
Reverse: AAGGCGTCATCCAGGCAC | ||
PtDib | Forward: GGCAAACACTGGTGGGAACT | KM880023 |
Reverse: ACCCTTCACGCCTCATCTTG | ||
PtETH | Forward: ATGCTCTCTGTTCTGGACTCAAG | MT890695 |
Reverse: TCACTTCTGCAGGTAACGCA | ||
β-actin | Forward: CGAAACCTTCAACACTCCCG | FI641977 |
Reverse: CGGGTTGACGAGACTGTTGG |
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Xie, X.; Zhang, J.; Tu, S.; Zhou, Q.; Zhu, D. Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus. Biology 2024, 13, 630. https://doi.org/10.3390/biology13080630
Xie X, Zhang J, Tu S, Zhou Q, Zhu D. Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus. Biology. 2024; 13(8):630. https://doi.org/10.3390/biology13080630
Chicago/Turabian StyleXie, Xi, Jun Zhang, Shisheng Tu, Qi Zhou, and Dongfa Zhu. 2024. "Corazonin Stimulates Ecdysteroid Synthesis during the Molting Process of the Swimming Crab, Portunus trituberculatus" Biology 13, no. 8: 630. https://doi.org/10.3390/biology13080630