Ablation of TrkB from Enkephalinergic Precursor-Derived Cerebellar Granule Cells Generates Ataxia
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Tissue Processing
2.3. Immunofluorescence
2.4. Estimation of a Proportion of GCs and PCs Undergoing Recombination
2.5. PC Count Analysis
2.6. Nissl Staining and Cavalieri Analysis
2.7. Immunoblotting
2.8. Behavioural Analysis
2.8.1. Hindlimb Clasping
2.8.2. Kyphosis Test
2.8.3. Ledge Test
2.8.4. Catwalk Test
2.9. Statistical Analysis
3. Results
3.1. Exploring the Impact of Ntrk2 Deletion in a Specific Subset of Cerebellar Granule Cells
3.2. Pattern of BAC-Penk-Cre Mediated Recombination in the Cerebellum
3.3. Unaltered Cerebellar Morphology of TrkbPenk-KO Mice
3.4. Molecular Consequences of Ntrk2 Deletion in TrkbPenk-KO Mice
3.5. Behavioural Consequences of Ntrk2 Deletion in Enk-Derived GCs
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Atoh1 | Pax6 | Penk+ Glutamatergic Progenitors, GC Precursors and GCs | |
---|---|---|---|
Total cells | 10,706 | 12,538 | 169 |
Penk | 187 | 162 | N/A |
Penk % of cells | 1.75 | 1.29 | N/A |
Ntrk2 | 1352 | 2686 | 25 |
Ntrk2 % of cells | 12.63 | 21.42 | 14.79 |
GCs | Golgi Cells | PCs | |
---|---|---|---|
Total cells | 477,176 | 3989 | 16,634 |
Penk scaled expression | 0.01 | 3.85 | 0.02 |
Penk % of cells | 1.03 | 81.35 | 2.03 |
Ntrk2 scaled expression | 1.48 | 11.17 | 0.4 |
Ntrk2 % of cells | 69.47 | 99 | 26.72 |
Test | Genotype | Age (in Months) | n | Median | Max | Min | Mann-Whitney U | Adjusted p |
---|---|---|---|---|---|---|---|---|
Hindpaw clasping | Control | 3 | 9, all male | 0 | 0 | 0 | 40.50 | >0.999 |
Mutant | 3 | 9, all male | 0 | 0 | 0 | |||
Control | 8 | 3, 1 male and 2 females | 0 | 0 | 0 | 6.00 | >0.999 | |
Mutant | 8 | 4, 2 males and 2 females | 0 | 0 | 0 | |||
Kyphosis test | Control | 3 | 10, 3 males and 7 females | 0 | 0 | 0 | 45.00 | >0.999 |
Mutant | 3 | 9, 4 males and 5 females | 0 | 0 | 0 | |||
Control | 8 | 6, 1 male and 5 females | 0 | 0 | 0 | 33.00 | >0.999 | |
Mutant | 8 | 12, 4 males and 8 females | 0 | 2 | 0 |
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Eliseeva, E.; Malik, M.Y.; Minichiello, L. Ablation of TrkB from Enkephalinergic Precursor-Derived Cerebellar Granule Cells Generates Ataxia. Biology 2024, 13, 637. https://doi.org/10.3390/biology13080637
Eliseeva E, Malik MY, Minichiello L. Ablation of TrkB from Enkephalinergic Precursor-Derived Cerebellar Granule Cells Generates Ataxia. Biology. 2024; 13(8):637. https://doi.org/10.3390/biology13080637
Chicago/Turabian StyleEliseeva, Elena, Mohd Yaseen Malik, and Liliana Minichiello. 2024. "Ablation of TrkB from Enkephalinergic Precursor-Derived Cerebellar Granule Cells Generates Ataxia" Biology 13, no. 8: 637. https://doi.org/10.3390/biology13080637