An In Vitro Orbital Flow Model to Study Mechanical Loading Effects on Osteoblasts
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Osteoblasts Cultures
2.2. Orbital Shaker and Rocker Flow Models
2.3. Osteoblast Proliferation Assay
2.4. Differentiation Assay
2.5. Gene Expression
2.6. Immunofluorescence Labeling of Cells
2.7. Statistical Analysis
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Mohan, S.; Surisetty, R.; Chandrasekhar, K. An In Vitro Orbital Flow Model to Study Mechanical Loading Effects on Osteoblasts. Biology 2024, 13, 646. https://doi.org/10.3390/biology13090646
Mohan S, Surisetty R, Chandrasekhar K. An In Vitro Orbital Flow Model to Study Mechanical Loading Effects on Osteoblasts. Biology. 2024; 13(9):646. https://doi.org/10.3390/biology13090646
Chicago/Turabian StyleMohan, Subburaman, Ritika Surisetty, and Kesavan Chandrasekhar. 2024. "An In Vitro Orbital Flow Model to Study Mechanical Loading Effects on Osteoblasts" Biology 13, no. 9: 646. https://doi.org/10.3390/biology13090646