Protective Effect of Polysaccharides Isolated from Sargassum horneri against H2O2-Induced Oxidative Stress Both In Vitro, in Vero Cells, and In Vivo in Zebrafish
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Chemicals and Regents
2.2. Plant Material and Sample Preparation
2.3. The Cell Cultures
2.4. The Assay for Cytotoxicity
2.5. Measurement of Intracellular ROS Levels
2.6. The Effect of SHP on Intracellular MDA Content and SOD Activity
2.7. Maintenance of Zebrafish
2.8. The Survival Rate of Zebrafish Embryos
2.9. Measurement of Oxidative Stress-Related Indicators (SOD, CAT, GSH-PX, and MDA) in Zebrafish
2.10. Determination of ROS in Zebrafish
2.11. Statistical Analysis
3. Results
3.1. FTIR Spectrum of S. horneri Polysaccharide (SHP)
3.2. Determination of the H2O2 Damage Model
3.3. The Effect of SHP against Oxidative Stress Induced by H2O2 in Vero Cells
3.4. The Scavenging Effect of SHP on ROS in Vero Cells
3.5. Effects of SHP on Intracellular MDA Content and SOD Activity
3.6. Effects of H2O2 on Survival Rate and Hatching Rate of Zebrafish
3.7. Effects of H2O2 on the Growth and Development of Zebrafish
3.8. Effects of SHP on H2O2-Induced Zebrafish
3.9. Effects of SHP on the Activities of SOD and CAT and on the Levels of GSH-PX and MDA in Zebrafish
3.10. The Role of SHP on H2O2-Induced ROS in Zebrafish
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample | (μg/mL) | Vero | |
---|---|---|---|
SOD (U/mgprot) | MDA (nmol/mgprot) | ||
Control | - | 52.43 ± 1.31 | 7.17 ± 1.29 |
H2O2 | - | 22.9 ± 4.46 ## | 19.04 ± 2.53 ## |
SHP | 25 | 28.28 ± 5.77 | 14.09 ± 0.51 ** |
50 | 30.80 ± 6.04 | 11.09 ± 2.10 ** | |
100 | 40.70 ± 8.95 ** | 9.39 ± 0.92 ** |
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Wei, S.; Wang, L.; Yang, J.; Xu, R.; Jia, R.; He, P. Protective Effect of Polysaccharides Isolated from Sargassum horneri against H2O2-Induced Oxidative Stress Both In Vitro, in Vero Cells, and In Vivo in Zebrafish. Biology 2024, 13, 651. https://doi.org/10.3390/biology13090651
Wei S, Wang L, Yang J, Xu R, Jia R, He P. Protective Effect of Polysaccharides Isolated from Sargassum horneri against H2O2-Induced Oxidative Stress Both In Vitro, in Vero Cells, and In Vivo in Zebrafish. Biology. 2024; 13(9):651. https://doi.org/10.3390/biology13090651
Chicago/Turabian StyleWei, Shuangyan, Li Wang, Jia Yang, Ruihang Xu, Rui Jia, and Peimin He. 2024. "Protective Effect of Polysaccharides Isolated from Sargassum horneri against H2O2-Induced Oxidative Stress Both In Vitro, in Vero Cells, and In Vivo in Zebrafish" Biology 13, no. 9: 651. https://doi.org/10.3390/biology13090651