Next Article in Journal
The Genus Chaetogaster Baer, 1827 (Annelida, Clitellata) in Switzerland: A First Step toward Cataloguing Its Molecular Diversity and Description of New Species on a DNA Sequence Basis
Previous Article in Journal
Differential Cellular Response to Mercury in Non-Farmed Fish Species Based on Mitochondrial DNA Copy Number Variation Analysis
Previous Article in Special Issue
Targeted Overexpression of Claudin 11 in Osteoblasts Increases Trabecular Bone Mass by Stimulating Osteogenesis at the Expense of Adipogenesis in Mice
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biology
Volume 13
Issue 9
10.3390/biology13090692
biology-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles thumb_up
...
Endorse textsms
...
Comment
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Review

Roles of Toll-like Receptor Signaling in Inflammatory Bone Resorption

by
Tsukasa Tominari
1,
Chiho Matsumoto
1,
Yuki Tanaka
2,
Kensuke Shimizu
1,
Masaru Takatoya
2,
Moe Sugasaki
2,
Kento Karouji
2,
Urara Kasuga
1,
Chisato Miyaura
1,
Shinji Miyata
3,
Yoshifumi Itoh
3,4,
Michiko Hirata
1 and
Masaki Inada
1,2,3,*
1
Department of Biotechnology and Life Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei-shi, Tokyo 184-8588, Japan
2
Cooperative Major of Advanced Health Science, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei-shi, Tokyo 184-8588, Japan
3
Inada Research Unit, Institute of Global Innovation Research, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei-shi, Tokyo 184-8588, Japan
4
Kennedy Institute of Rheumatology, Nuffield Department of Orthopaedics, Rheumatology and Musculoskeletal Sciences, University of Oxford, Oxford OX3 7FY, UK
*
Author to whom correspondence should be addressed.
Biology 2024, 13(9), 692; https://doi.org/10.3390/biology13090692
Submission received: 30 July 2024 / Revised: 26 August 2024 / Accepted: 29 August 2024 / Published: 4 September 2024
(This article belongs to the Special Issue Bone Cell Biology)
Browse Figures
Versions Notes

Abstract

:

Simple Summary

Toll-like receptor (TLR) signaling contributes to the pathogenesis of inflammatory oral diseases, such as periodontal disease by stimulating osteoclast differentiation and function. Recent reports suggest that various components from bacteria, viruses, and autologous cells act as TLR ligands. Lipopolysaccharide (LPS), a TLR4 ligand, induces osteoclast differentiation and bone resorption; however, blocking PGE2 synthesis or antagonizing PGE2 receptor signaling abrogates LPS-induced inflammatory bone resorption. In addition, other ligands for TLR2/1, TLR2/6 and TLR3 facilitate PGE2 production, leading to osteoclastic bone resorption. This review introduces the latest findings regarding the relationship between TLR signaling pathways and inflammatory bone resorption.

Abstract

Toll-like receptors (TLRs) are pattern recognition receptors expressed in immune cells, including neutrophils, macrophages, and dendritic cells. Microbe-associated molecular patterns, including bacterial components, membranes, nucleic acids, and flagella are recognized by TLRs in inflammatory immune responses. Periodontal disease is an inflammatory disease known to cause local infections associated with gingival inflammation, subsequently leading to alveolar bone resorption. Prostaglandin E2 (PGE2) is a key mediator of TLR-induced inflammatory bone resorption. We previously reported that membrane-bound PGE synthase (mPGES-1)-deficient mice failed to induce bone resorption by lipopolysaccharide (LPS), a major pathogenic factor involved in periodontal bone resorption. Further experiments exploring specific pathogen-promoting osteoclast differentiation revealed that various TLR ligands induced osteoclast differentiation in a co-culture model. The ligands for TLR2/1, TLR2/6, TLR3, and TLR5, as well as TLR4, induce osteoclast differentiation associated with the production of PGE2 and the receptor activator of nuclear factor-kappa B ligand (RANKL), an inevitable inducer of osteoclast differentiation in osteoblasts. In vivo, local injection of TLR ligands, including TLR2/1, TLR2/6, and TLR3, resulted in severe alveolar bone resorption. This review summarizes the latest findings on TLR-mediated osteoclast differentiation and bone resorption in inflammatory diseases, such as periodontal diseases.

1. Introduction

Toll-like receptors (TLRs) are pattern recognition receptors (PRRs) that recognize microbe-associated molecular patterns (MAMPs), also known as pathogen-associated molecular patterns (PAMPs), and damage-associated molecular patterns (DAMPs) that initiate innate responses in immune cells, including neutrophils, macrophages, and dendritic cells [1].
Thus far, 10 TLRs have been identified in humans (TLR1-10) and 12 TLRs in mice (TLR1-9 and 11-13) [2]. The TLR family can be divided into cell surface and intracellular. TLR1, TLR2, TLR4, TLR5, and TLR6 are mainly located on the cell surface of the plasma membranes, whereas TLR3, TLR7, TLR8, and TLR9 are primarily located on the intracellular membranes of endosomes, phagosomes, and lysosomes. All TLRs except TLR3 activate the myeloid differentiation factor 88 (MyD88)-dependent pathway. In contrast, TLR3 and TLR4 activate toll/interleukin-1 receptor-domain-containing adapter-inducing interferon-β (TRIF)-dependent pathways. Because TLR4 is internalized into the cytosol and located on intracellular membranes, it transduced both MyD88-dependent and TRIF-dependent signaling pathways. These pathways stimulate nuclear factor-κB (NF-κB), mitogen-activated protein kinases (MAPKs), and interferon regulatory factors (IRFs) to produce inflammatory cytokines and type I interferons (IFNs) (Figure 1). TLRs mediate several cases of oral infection, such as hard tissue breakdown of alveolar bones in periodontal disease.
Bone metabolism is precisely regulated by the balance between osteoclastic bone resorption and osteoblastic bone formation, known as bone remodeling [3]. Various factors, including inflammatory cytokines, hormones, mechanical unloading, and aging, induce excessive osteoclastic bone resorption, resulting in decreased bone mineral density (BMD) and bone volume [4,5]. Osteoclasts are multinucleated bone-resorbing cells that differentiate from macrophage-lineage cells. In contrast, osteoblasts play a dual role in forming new bone through the secretion of bone substrates and the induction of osteoclast differentiation through the expression of receptor activator of NF-κB ligand (RANKL). The interaction between RANKL and RANK expressed on osteoclast precursor cells stimulates osteoclast differentiation by activating several pathways, such as the nuclear factor of activated T cells (NFATc1), NF-κB, and activator protein (AP)-1 [6].
Periodontal disease is an inflammatory disease characterized by a bacterial infection that results in the progression of alveolar bone resorption and tooth loss. Lipopolysaccharide (LPS), an outer membrane component of Gram-negative bacteria, is a well-characterized TLR4 ligand that induces inflammatory bone resorption in periodontal diseases. Various bacterial and virus components and cellular components from dead cells are identified as exogenous and endogenous TLR ligands, respectively [7,8,9]. Because these ligands are considered to be abundant in infiltrated tissues, TLR signaling can be excessively activated and induce inflammatory bone resorption.
In this review, we summarize the role of TLRs in inflammatory bone resorption.

2. Prostaglandin E2 Is a Primary Mediator of Inflammatory Bone Resorption

PGE2 is a primary inflammatory mediator of lipid metabolites that is synthesized via the arachidonic acid (AA) cascade and is a potent inducer of osteoclastic bone resorption in inflammatory bone-related diseases, such as periodontitis and arthritis [10,11,12]. The AA cascade proceeds through metabolic processes: cell membrane phospholipids release AA via phospholipase A2 (PLA2). AA is converted to PGG2 and PGH2 by cyclooxygenases (COXs), and PGE synthases produce PGE2 from PGH2. PGE2 receptors are classified into four G-protein-coupled receptor subtypes, EP1-4. PGE2 synthesis (production) is stimulated by several cytokines, such as interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α), through the upregulation of cyclooxygenase 2 (COX2) and membrane-bound PGE synthase-1 (mPGES-1) [13].
We herein report a role for PGE2 in inflammatory bone resorption. Adding PGE2 or each agonist to EP2 and EP4, but not EP1 and EP3, induced osteoclast differentiation in mouse bone marrow cultures and bone resorption in mouse calvarial organ cultures [14]. In addition, PGE2-induced bone resorption was attenuated only in EP4 knockout mice, not in EP1-3 knockout mice, in mouse calvarial organ cultures [15]. Other studies have consistently reported that PGE2 enhances RANKL-induced osteoclast differentiation [16,17]. In fact, in an animal model of periodontitis, the blockage of PG biosynthesis by indomethacin suppressed alveolar bone resorption [18,19]. We also clarified the crosstalk between TLR signaling and PGE2 in osteoclast differentiation [11,20,21,22].

3. Roles of TLR Signaling in Osteoclast Differentiation

3.1. Exogenous and Endogenous Ligands for TLRs

Various MAMPs and DAMPs are identified as TLR ligands (Table 1). TLRs form hetero- or homodimers to activate downstream signaling pathways (Figure 1). TLR2/1, TLR2/6, and TLR4 recruit cytoplasmic TIR-domain-containing adaptor protein (TIRAP) and activate the MyD88-dependent pathway. TLR5, TLR7, TLR8, and TLR9 use the MyD88-dependent pathway, whereas TLR3 uses a TRIF-dependent pathway. In particular, TLR4 is endocytosed and translocated on an endosomal membrane, similar to TLR3, TLR7, TLR8, and TLR9, to activate the TRIF-related adaptor molecule (TRAM) and TRIF-dependent pathways. The MyD88-dependent pathway drives the induction of proinflammatory cytokines via NF-κB and AP-1, whereas the TRIF-dependent pathway drives type 1 IFNs via IRF3 and IRF7. Proinflammatory cytokines such as IL-1 and tumor necrosis factor-α (TNF-α) are positive regulators of osteoclast differentiation and function by acting on osteoblasts, osteoclast precursor cells, and mature osteoclasts [23,24]. Type 1 interferons, such as IFN-α and IFN-β, are negative regulators of osteoclast differentiation and function [25].

3.2. Roles of Cell Surface and Intracellular TLR4 for LPS from Gram-Negative Bacteria

LPS is a bacterial endotoxin that is an outer membrane component of Gram-negative bacteria and is a major pathogenic factor in periodontal disease. TLR4 is a well-characterized LPS receptor. Gram-negative bacteria are found in subgingival plaques deep in the periodontal pocket. Periodontal pathogens such as Aggregatibacter actinomycetemcomitans and P. gingivalis possess LPS and cause severe inflammatory responses when they infect periodontal tissues. TLR4 requires an accessory protein, myeloid differentiation protein 2 (MD2), to recognize LPS. LPS-binding protein (LBP) forms a complex with LPS, binds to CD14, and is subsequently delivered to the TLR4-MD2 complex to activate TLR4 signaling [26]. Cell surface TLR4-MyD88-dependent signaling leads to early NF-κB activation. At this point, TLR4 is subsequently endocytosed, which is controlled by CD14, and intracellular (endosomal) TLR4 transduces TRIF-dependent signaling, resulting in late NF-κB activation [27]. We previously demonstrated that LPS upregulated the mRNA expression of Ptgs2 (COX-2) and Ptges (mPGES-1) and promoted PGE2 synthesis in osteoblasts, inducing osteoclast differentiation through PGE2-mediated Tnfsf11 (RANKL) upregulation in osteoblasts [28]. Intraperitoneal injection of LPS into cytosolic PLA2α (cPLA2α)-deficient mice resulted in decreased PGE2 levels in BMCs but failed to decrease femoral BMD [28]. Similarly, local injection of LPS into mouse mandibular gingiva induced alveolar bone resorption characterized by a decrease in alveolar BMD, whereas LPS injection did not decrease alveolar BMD in mPGES-1-deficient mice [11]. We further confirmed that blocking PGE2 production using several compounds and natural phytochemicals, including β-cryptoxanthin [29,30], lutein [31], epigallocatechin gallates [32,33], and polymethoxyflavones [34,35], significantly suppressed LPS-induced osteoclast differentiation and alveolar bone resorption. Based on these data, we suggest that LPS targets osteoblasts expressing TLR4 and stimulates PGE2 production by upregulating Ptgs2 and Ptges mRNA expression via the LPS/TLR4/NF-κB pathway. PGE2 binds to EP4 via paracrine and autocrine signaling in osteoblasts to induce RANKL expression, supporting osteoclast differentiation (Figure 2).
LPS also directly controls the functions of osteoclast precursor cells and mature osteoclasts. Several reports have shown that LPS plays a dual role in osteoclast differentiation, inducing osteoclast differentiation without RANKL [36,37], promoting osteoclast differentiation via the TLR4/TNF-α axis in RANKL-primed cells [38,39], and prolonging the lifespan of mature osteoclasts [40,41]. In contrast, LPS suppresses RANKL-induced osteoclast differentiation [39,40]. Itoh et al. [41] reported that the LPS-mediated survival of osteoclasts does not mediate the production of cytokines, including IL-1 and TNF-α, osteoprotegerin (OPG), and macrophage colony-stimulating factor (M-CSF), indicating that the LPS-TLR4-dependent survival of osteoclasts due to LPS-TLR4 is mediated by the TRAF6-NF-κB and PI3K-AKT pathways.
The role of internal TLRs in host cells is currently understood to be the induction of inflammation. Several DAMPs derived from dead cells have been reported to function as endogenous TLR4 ligands, including high-mobility group box 1 (HMGB1) and S100 proteins, which induce osteoclastic bone resorption [42,43,44]. HMGB1 can also bind to TLR2 and TLR5, which activate inflammatory responses [45]. These in vivo and in vitro studies demonstrate that TLR4 signaling driven by LPS and endogenous factors is a potent inducer of bone resorption through an indirect effect mediated by RANKL expression in osteoblasts and a direct effect that extends the lifespan of osteoclasts (Figure 2).

3.3. Cell Surface TLR2 Heterodimers as Receptors for Bacterial Cell-Wall Components from Gram-Positive and Gram-Negative Bacteria

TLR2 forms heterodimers with TLR1 or TLR6 to recognize lipopeptides and lipoteichoic acids (LTAs) of Gram-positive or Gram-negative bacteria membrane components and activates MyD88-mediated signaling [46].
Gram-positive bacteria are found in supragingival plaques, in contrast to Gram-negative bacteria. TLR2/1 heterodimers recognize bacterial triacylated lipopeptides, whereas the TLR2/6 heterodimer recognizes bacterial diacylated lipopeptides, such as LTA, macrophage-activating lipopeptide 2 (MALP2) from Mycoplasma fermentans, FSL-1 from M. salivarium, and zymosan, a β-glucan-rich product found in the yeast cell wall [46]. We previously reported that synthetic ligands for TLR2/1 (Pam3CSK4) and TLR2/6 (Pam2CSK4) induce osteoclast differentiation through PGE2 production in osteoblasts [20]. LTA derived from the cell wall of Gram-positive bacteria, a natural ligand for TLR2/6, induces PGE2-mediated osteoclast differentiation, whereas indomethacin treatment suppresses these activities [21], and TLR2 ligands extend the lifespan of mature osteoclasts [20,21]. Local injection of these ligands for TLR2 into the mandibular gingiva induces alveolar bone resorption in mice [20,21]. Based on these data, we suggest that TLR2 signaling, similar to TLR4 signaling in osteoblasts, is involved in PGE2-mediated osteoclast differentiation (Figure 2). Yano et al. reported that the TLR2/1 ligand (Pam3CSK4) directly induces osteoclast differentiation in Raw264.7 cells [47]. A recent report indicated that sialylated TLR2 functions as a receptor for sialic acid-binding immunoglobulin-type lectin 15 (Siglec15), which is identified as a ligand for DNAX-activating protein 12 kDa (DAP12) and initiates cell-to-cell fusion between preosteoclasts for osteoclast differentiation [48]. Thus, TLR2 signaling positively regulates the osteoclast differentiation and function.

3.4. Cell Surface TLR5, a Receptor for Flagellum from Gram-Positive and G-Negative Bacteria

Flagellin, a component of the flagellum of Gram-positive and Gram-negative bacteria, is recognized by TLR5. Ha et al. reported that TLR5 activation by flagellin suppresses RANKL-induced osteoclast differentiation by inhibiting c-Fos expression, a key regulator of osteoclast differentiation, and promoting interferon-β (IFN-β) production in bone marrow-derived macrophage cultures, whereas flagellin induces osteoclast differentiation in co-cultures of osteoblasts and bone marrow cells without inducing IFN-β production [49]. Kassem et al. reported that TLR5 activation by a local injection of flagellin over the skull bone in mice induces osteoclast differentiation and bone resorption associated with increased Tnfsf11 (RANKL) mRNA expression [50]. Chamberlain et al. reported that potential endogenous TLR5 ligands, such as lectin and HSPs, promote TNF-α production from RA fibroblasts and macrophages to exacerbate RA progression [51]. Kim et al. reported that intra-articular injection of flagellin promoted TNF-α production through monocyte infiltration and exacerbated arthritic bone erosion in collagen-induced arthritis model mice [52]. These reports suggest that TLR5 signaling may be involved in periodontal alveolar bone resorption.

3.5. Intracellular TLR3, 7, and 9 as Receptors for Nucleic Acids Including Endogenous and Exogenous RNA and DNA

There are few reports on the roles of TLR3, 7, and 9 expressed on endosomal/lysosomal membranes in bone resorption. Mouse TLR8 has been suggested to be non-functional or to partially control TLR7 expression [53]. Kim et al. reported that poly(I:C) upregulates RANKL expression in RA fibroblast-like synoviocytes (FLS), and co-culture of human monocytes with TLR3-activated RA-FLS induces osteoclast differentiation [54]. We previously demonstrated that poly(I:C), a synthetic dsRNA analog, is endocytosed by osteoblasts and upregulates RANKL expression, inducing osteoclast differentiation [22]. Indomethacin and an EP4 antagonist blocked this effect, suggesting that PGE2 mediates poly(I:C)-TLR3 signaling-induced osteoclast differentiation (Figure 2) [22]. TLR3 also recognizes RNA from dead cells, mitochondrial dsRNA, and viral dsRNA [55,56,57]. Because these RNAs are derived from host dead cells, including necrotic cells, apoptotic cells, and immune cells, releasing antimicrobial extracellular traps, and infected bacteria may accumulate in inflamed tissues, TLR3 signaling may be involved in inflammatory bone resorption in periodontitis and others.
The synthetic TLR7 ligand, B848 (resiquimod) or bropirimine, has been reported to inhibit RANKL-induced osteoclast differentiation, but not the survival or function, in BMM cultures and human PBMC cultures [58] and suppresses di-hydroxyvitamin D3 [1,25(OH)2D3]-induced osteoclast differentiation in co-cultures of bone marrow cells and osteoblasts through IFN-β production [59]. Several reports have shown that TLR7 signaling contributes to inflammatory bone resorption associated with RA. Alzabin et al. reported that TLR7-deficient mice showed milder pathology of collagen-induced arthritis than wild-type mice [60]. TLR7 activated by single-stranded RNA from RA synovial fluid has been reported to promote osteoclast differentiation by upregulating RANKL expression in synovial fibroblasts [61]. Hegewald et al. reported that miR-574-5p delivered by small extracellular vesicles secreted from synovial fibroblasts in RA patients induces osteoclast differentiation via TLR7/8 signaling [62].
Synthetic oligonucleotides containing unmethylated CpG dinucleotides (CpG-ODNs) promote osteoclast differentiation from RANKL-pretreated BMMs via the induction of TNF-α, whereas CpG-ODN inhibits osteoclast differentiation from BMMs via the downregulation of M-CSF receptor at the early stage of differentiation [63,64]. Ding et al. reported that osteoclast differentiation is promoted in cell cultures of TLR9-deficient BMMs treated with M-CSF and RANKL, and TLR9-deficient mice exhibit low bone mass due to the promotion of osteoclast differentiation, which is associated with chronic systemic inflammation [65]. Kim et al. demonstrated that TLR9 signaling elevates proinflammatory cytokine production, including IL-6 and TNF-α, and mediates inflammatory alveolar bone loss induced by P. gingivalis infection in mice [66].
These reports suggest that intracellular TLRs may be involved in the pathogenesis or progression of periodontal bone resorption by recognizing bacterial or host cell-derived nucleic acids.

3.6. TLR Ligands Bind TLRs and Coordinate the Inflammatory Signaling with Other Family of Cytosolic Sensor

Several TLR ligands are incorporated into the cytosol of host cells through endocytosis of bacterial extracellular vesicles or a type III secretory system by certain bacteria and are recognized by cytosolic PRRs, which activate TLR-independent signaling. LPS is one of the components of bacterial outer membrane vesicles (OMVs) secreted from Gram-negative bacteria, and OMVs are endocytosed by cells, delivering LPS into the cytosol [67,68]. Intracellular LPS directly binds to caspase-11, which cooperates with NOD-like receptor protein 3 (NLRP3) to trigger caspase-11-dependent cell death and IL-1β responses. Flagellin is also delivered to the cytosol via the type III secretory system [69]. Intracellular flagellin is sensed by the NOD-like receptor family caspase recruitment domain-containing protein 4 (NLRC4), which activates the NLRC4 inflammasome and caspase-1-mediated IL-1β secretion [70]. Recent studies have shown that NLRP3 and NLRC4 inflammasomes positively regulate osteoclast differentiation and bone resorption [71,72].
In contrast, RNA and DNA are sensed by cytosolic sensors after endosomal escape. Retinoic acid-inducible gene-I (RIG-I) and melanoma differentiation-associated protein 5 (MDA5) preferentially recognize short (<300 bp) and long (>1000 bp) dsRNAs, respectively, leading to mitochondrial antiviral signaling (MAVS)-dependent NF-κB and IRF activation [73]. The cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING) pathway senses cytosolic DNA, including mitochondrial DNA, as well as bacteria and dead cells, which activate the transcription of NF-κB and IRF [74]. MacLauchlan et al. reported that STING-deficient mice show bone loss over time due to IFN-dependent suppression of osteoclast differentiation [75].
Further studies are required to clarify the crosstalk between TLRs and cytosolic sensors activated by TLR ligands that regulate bone resorption.

3.7. Roles of Immune Cells Activated by TLR Ligands in Inflammatory Bone Resorption

Various immune cells are infiltrated and accumulated in infected periodontal tissues to regulate local immune responses. Li et al. profiled and analyzed the subtypes of immune cells present in periodontitis tissues (n = 210) compared to healthy periodontal tissues (n = 133) using datasets from the Gene Expression Omnibus (GEO) [76]. They showed that plasma cells (differentiated B cells), naïve B cells, and neutrophils are elevated in periodontitis tissues, whereas memory B cells, CD4+ memory T cells, dendritic cells, mast cells and M1/M2 macrophages are more prevalent in healthy tissues. These immune cells can contribute to osteoclast differentiation by producing inflammatory cytokines and RANKL in inflammatory conditions [77,78,79,80]. Several reports demonstrated that neutrophils, B cells and T cells accumulated in periodontal lesions produce inflammatory mediators [81,82], including TNF-α, IL-1, PGE2 and oncostatin. M. Makkawi et al. demonstrated that TLR2/PI3K signaling activated by P. gingivalis in neutrophils and macrophages facilitates the production of inflammatory cytokines [83]. Inflammatory mediators support osteoclast differentiation and alveolar bone resorption through upregulating RANKL expression in osteogenic cells, such as osteoblasts and periodontal ligament cells, and/or directly activating osteoclasts. Chakravarti et al. reported that several TLR ligands for TLR4 (LPS), TLR2 (Pam3CSK4), TLR5 (flagellin) and TLR7 (gardiquimod) induce RANKL expression in neutrophils, and osteoclast differentiation is induced in co-cultures of monocytes and LPS-activated neutrophils without the addition of exogenous RANKL [77]. Also, neutrophil extracellular traps (NETs), which are net-like structures composed of DNA and antimicrobial proteins to trap and kill pathogens, have been reported to exacerbate alveolar bone resorption in periodontal disease [84,85]. TLR ligands for TLR2/6, TLR4, TLR7, TLR8 and TLR9 induce NET formation through NADPH oxidase (NOX)-dependent ROS production [86]. On the other hand, some immune cells, including regulatory B cells (Bregs), regulatory T cells and CD8+ T cells, have been reported to inhibit inflammatory alveolar bone resorption [87,88,89].
Overall, in immune-bone interaction, certain immune cells promote bone resorption and others inhibit it, indicating that a disrupted balance of immune cell populations in periodontitis tissues contributes to the development of periodontal bone resorption.

4. Conclusions

Current scientific progress in the field of infectious inflammation and TLRs has revealed the inevitable roles of TLR signaling in inflammatory bone resorption of periodontal disease. LPS derived from Gram-negative bacteria is a major pathogen of periodontal alveolar bone resorption via TLR4 signaling, and other TLRs contribute to the elongation of TLR4 functions. TLR ligands from bacterial membranes, including LPS and LTA (TLR2/1, TLR2/6, and TLR4 ligands), flagellin (TLR5 ligand), and nucleic acids (TLR3, TLR,7, and TLR9 ligands), are cross-sectionally recognized by all TLRs. Endogenous factors, such as DAMPs from dead cells and components of extracellular traps from immune cells recruited to inflamed tissues, are also recognized by TLRs. These TLR ligands are also sensed by cytosolic sensors, such as inflammasomes, RIG-I/MDA5, and cGAS-STING. The signaling networks in the pathogenic bone and surrounding tissues are presumed to be highly active and increase inflammation and subsequent inflammatory bone resorption (Figure 3).
The role of TLR signaling in osteoclasts remains controversial. Osteoclast precursors express TLR1-9, especially TLR2 and TLR4, which are the major TLRs induced by RANKL in mature osteoclasts [21,22,26]. We previously screened which TLR ligands induced osteoclast differentiation in co-cultures of mouse primary osteoblasts (POBs) and bone marrow cells (BMCs) among TLR2/1 (Pam3CSK4), TLR2/6 (fibroblast-stimulating lipopeptide 1; FSL-1), TLR3 (poly[I:C]), TLR4 (LPS from Escherichia coli and Porphyromonas gingivalis), TLR5 (flagellin from Salmonella typhimurium), TLR7 (single-strand RNA 40), and TLR9 (CpG oligonucleotide; ODN1826). The ligands for TLR2/1, TLR2/6, TLR3, TLR4, and TLR5 significantly induced osteoclast differentiation (Figure 2). The inhibitory effects of TLR ligands have been reported for TLR2 (peptidoglycan from Staphylococcus aureus), TLR3 (poly[I:C]), TLR4 (LPS from E. coli), and TLR9 (synthetic phosphothioate-stabilized CpG DNA), which strongly inhibited RANKL-induced osteoclast differentiation despite stimulation of tumor necrosis factor-α (TNF-α) in mouse bone marrow macrophage (BMM) cultures and the mouse macrophage lineage cell line Raw264.7 [26]. In contrast, TLR2 and TLR4 activation by each ligand has been shown to enhance the survival of mature osteoclasts in BMM cultures [26]. These reports suggest that several TLRs play different roles in inflammatory bone resorption, including in periodontitis. Further investigation is needed to elucidate the precise role of TLRs in osteoclast differentiation.

5. Future Perspectives

Bacterial infection activates TLR signaling and other cytosolic sensor signaling through bacterial components and autologous DAMPs released from host-derived immune cells and dead cells in the periodontal tissues. These factors participate in the development and progression of inflammatory bone resorption (Figure 3). Further research is needed to elucidate the interaction networks of each TLR, including both endosomal/lysosomal TLRs and other cytosolic sensor families, activated by exogenous and endogenous ligands originating from bacteria and host cells. These findings concerning TLR function are expected to contribute to effective drug discovery for progressive periodontal diseases.

Author Contributions

Conceptualization, T.T., C.M. (Chiho Matsumoto) and M.I.; investigation, T.T., C.M. (Chiho Matsumoto), Y.T., K.S., M.T., M.S., K.K. and U.K.; writing—original draft preparation, T.T., C.M. (Chiho Matsumoto) and M.I.; writing—review and editing, C.M. (Chisato Miyaura), S.M., Y.I., M.H. and M.I.; visualization, T.T.; supervision, M.I.; project administration, C.M. (Chisato Miyaura) and M.I. All authors have read and agreed to the published version of the manuscript.

Funding

This work was supported by a Grant-in-Aid from the Japan Society for the Promotion of Science (JSPS) KAKENHI (M.I. and M.H.: 22K05490).

Institutional Review Board Statement

Not applicable.

Informed Consent Statement

Not applicable.

Data Availability Statement

The raw data supporting the conclusions of this article will be made available by the authors on request.

Conflicts of Interest

The authors declare no conflicts of interest.

Abbreviations

Abbreviation or AcronymName
1,25(OH)2D31,25-dihydroxyvitamin D3
AAArachidonic acid
AGEAdvanced glycation end product
A. ActinomycetemcomitansAggregatibacter actinomycetemcomitans
Anti-MPAntimicrobial peptide
AP-1Activator protein-1
BMCBone marrow cell
BMDBone mineral density
CDCluster of differentiation
cGASCyclic GMP-AMP synthase
COXCyclooxygenase
ODNOligodeoxynucleotide
DAMPDamage-associated molecular pattern
DAP12DNAX activating protein 12 kDa
dsRNADouble strand RNA
E. coliEscherichia coli
EPProstaglandin E2 receptor
FLSFibroblast-like synoviocyte
FSL-1Fibroblast-stimulating lipopeptide
GEOGene Expression Omnibus
HMGB1High mobility group box 1
HSPHeat shock protein
IFNInterferon
IκBαInhibitor of NF-κB α
IKKIκB kinase
ILInterleukin
IRAKIL-1 receptor-associated kinase
IRFInterferon regulatory factor
LDLLow-density lipoprotein
LL37Cathelicidin-related antimicrobial peptide
LPSLipopolysaccharide
LTALipoteichoic acid
MAMPMicrobe-associated molecular pattern
MALP2Macrophage-activating lipopeptide 2
MAPKMitogen-activated protein kinase
M-CSFMacrophage colony-stimulating factor
MD2Myeloid differentiation protein 2
MDA5Melanoma differentiation-associated gene 5
mPGES-1Membrane-bound PGE synthase
MyD88Myeloid differentiation factor 88
NFATc1Nuclear factor of activated T cells, cytosolic 1
NETNeutrophil extracellular trap
NF-κBNuclear factor-κB
NLRCNOD-like receptor family caspase recruitment domain-containing protein 4
NLRPNOD-like receptor protein 3
NODNucleotide oligomerization domain
NOXNADPH oxidase
OMVBacterial outer membrane vesicle
OPGOsteoprotegerin
PAMPPathogen-associated molecular pattern
PGProstaglandin
PI3KPhosphatidylinositol-3 kinase
PLA2Phospholipase A2
P. gingivalisPorphyromonas gingivalis
PRRPattern recognition receptor
RARheumatoid arthritis
RANKReceptor activator of NF-κB
RANKLReceptor activator of NF-κB ligand
RIG-IRetinoic acid-inducible gene-I
RIPReceptor interacting protein
snRNASmall nuclear RNA
ssRNASingle-strand RNA
STINGStimulator of interferon genes
TIRAPToll/interleukin-1 receptor-domain-containing adapter protein
TLRToll-like receptor
TNFTumor necrosis factor
TRAFTNF receptor-associated factor
TRAMTRIF-related adaptor molecule
TRIFToll/interleukin-1 receptor-domain-containing adapter-inducing interferon-β

References

  1. Duan, T.; Du, Y.; Xing, C.; Wang, H.Y.; Wang, R.F. Toll-Like Receptor Signaling and Its Role in Cell-Mediated Immunity. Front. Immunol. 2022, 13, 812774. [Google Scholar] [CrossRef]
  2. Fitzgerald, K.A.; Kagan, J.C. Toll-like Receptors and the Control of Immunity. Cell 2020, 180, 1044–1066. [Google Scholar] [CrossRef] [PubMed]
  3. Bolamperti, S.; Villa, I.; Rubinacci, A. Bone remodeling: An operational process ensuring survival and bone mechanical competence. Bone Res. 2022, 10, 48. [Google Scholar] [CrossRef]
  4. Roodman, G.D. Regulation of osteoclast differentiation. Ann. N. Y. Acad. Sci. 2006, 1068, 100–109. [Google Scholar] [CrossRef]
  5. Moriishi, T.; Fukuyama, R.; Ito, M.; Miyazaki, T.; Maeno, T.; Kawai, Y.; Komori, H.; Komori, T. Osteocyte network; a negative regulatory system for bone mass augmented by the induction of Rankl in osteoblasts and Sost in osteocytes at unloading. PLoS ONE 2012, 7, e40143. [Google Scholar] [CrossRef]
  6. Park, J.H.; Lee, N.K.; Lee, S.Y. Current Understanding of RANK Signaling in Osteoclast Differentiation and Maturation. Mol. Cells. 2017, 40, 706–713. [Google Scholar] [CrossRef] [PubMed]
  7. Yu, L.; Wang, L.; Chen, S. Endogenous Toll-like Receptor Ligands and Their Biological Significance. J. Cell. Mol. Med. 2010, 14, 2592–2603. [Google Scholar] [CrossRef] [PubMed]
  8. Liu, G.; Zhao, Y. Toll-like Receptors and Immune Regulation: Their Direct and Indirect Modulation on Regulatory CD4+ CD25+ T Cells. Immunology 2007, 122, 149–156. [Google Scholar] [CrossRef] [PubMed]
  9. Reuter, S.; Herold, K.; Domroes, J.; Mrowka, R.; Schön, A. Toll-Like Receptor 5 as a Novel Receptor for Fungal Zymosan. Heliyon 2023. preprint. Available online: https://www.biorxiv.org/content/10.1101/2021.12.23.473960v1 (accessed on 29 July 2024). [CrossRef]
  10. McCoy, J.M.; Wicks, J.R.; Audoly, L.P. The Role of Prostaglandin E2 Receptors in the Pathogenesis of Rheumatoid Arthritis. J. Clin. Investig. 2002, 110, 651–658. [Google Scholar] [CrossRef]
  11. Inada, M.; Matsumoto, C.; Uematsu, S.; Akira, S.; Miyaura, C. Membrane-Bound Prostaglandin E Synthase-1-Mediated Prostaglandin E2 Production by Osteoblast Plays a Critical Role in Lipopolysaccharide-Induced Bone Loss Associated with Inflammation. J. Immunol. 2006, 177, 1879–1885. [Google Scholar] [CrossRef]
  12. Jiang, W.; Jin, Y.; Zhang, S.; Ding, Y.; Huo, K.; Yang, J.; Zhao, L.; Nian, B.; Zhong, T.P.; Lu, W.; et al. PGE2 Activates EP4 in Subchondral Bone Osteoclasts to Regulate Osteoarthritis. Bone Res. 2022, 10, 27. [Google Scholar] [CrossRef]
  13. Båge, T.; Kats, A.; Lopez, B.S.; Morgan, G.; Nilsson, G.; Burt, I.; Korotkova, M.; Corbett, L.; Knox, A.J.; Pino, L.; et al. Expression of Prostaglandin E Synthases in Periodontitis Immunolocalization and Cellular Regulation. Am. J. Pathol. 2011, 178, 1676–1688. [Google Scholar] [CrossRef] [PubMed]
  14. Suzawa, T.; Miyaura, C.; Inada, M.; Maruyama, T.; Sugimoto, Y.; Ushikubi, F.; Ichikawa, A.; Narumiya, S.; Suda, T. The Role of Prostaglandin E Receptor Subtypes (EP1, EP2, EP3, and EP4) in Bone Resorption: An Analysis Using Specific Agonists for the Respective EPs. Endocrinology 2000, 141, 1554–1559. [Google Scholar] [CrossRef] [PubMed]
  15. Miyaura, C.; Inada, M.; Suzawa, T.; Sugimoto, Y.; Ushikubi, F.; Ichikawa, A.; Narumiya, S.; Suda, T. Impaired Bone Resorption to Prostaglandin E2 in Prostaglandin E Receptor EP4-Knockout Mice. J. Biol. Chem. 2000, 275, 19819–19823. [Google Scholar] [CrossRef]
  16. Kobayashi, Y.; Mizoguchi, T.; Take, I.; Kurihara, S.; Udagawa, N.; Takahashi, N. Prostaglandin E2 Enhances Osteoclastic Differentiation of Precursor Cells through Protein Kinase A-Dependent Phosphorylation of TAK1. J. Biol. Chem. 2005, 280, 11395–11403. [Google Scholar] [CrossRef] [PubMed]
  17. Kaji, H.; Sugimoto, T.; Kanatani, M.; Fukase, M.; Kumegawa, M.; Chihara, K. Prostaglandin E2 Stimulates Osteoclast-like Cell Formation and Bone-resorbing Activity via Osteoblasts: Role of CAMP-dependent Protein Kinase. J. Bone Miner. Res. 1996, 11, 62–71. [Google Scholar] [CrossRef]
  18. Nyman, S.; Schroeder, H.E.; Lindhe, J. Suppression of Inflammation and Bone Resorption by Indomethacin During Experimental Periodontitis in Dogs. J. Periodontol. 1979, 50, 450–461. [Google Scholar] [CrossRef]
  19. Zubery, Y.; Dunstan, C.R.; Story, B.M.; Kesavalu, L.; Ebersole, J.L.; Holt, S.C.; Boyce, B.F. Bone Resorption Caused by Three Periodontal Pathogens In Vivo in Mice Is Mediated in Part by Prostaglandin. Infect. Immun. 1998, 66, 4158–4162. [Google Scholar] [CrossRef]
  20. Matsumoto, C.; Oda, T.; Yokoyama, S.; Tominari, T.; Hirata, M.; Miyaura, C.; Inada, M. Toll-like Receptor 2 Heterodimers, TLR2/6 and TLR2/1 Induce Prostaglandin E Production by Osteoblasts, Osteoclast Formation and Inflammatory Periodontitis. Biochem. Biophys. Res. Commun. 2012, 428, 110–115. [Google Scholar] [CrossRef]
  21. Tominari, T.; Sanada, A.; Ichimaru, R.; Matsumoto, C.; Hirata, M.; Itoh, Y.; Numabe, Y.; Miyaura, C.; Inada, M. Gram-Positive Bacteria Cell Wall-Derived Lipoteichoic Acid Induces Inflammatory Alveolar Bone Loss through Prostaglandin E Production in Osteoblasts. Sci. Rep. 2021, 11, 13353. [Google Scholar] [CrossRef] [PubMed]
  22. Tominari, T.; Akita, M.; Matsumoto, C.; Hirata, M.; Yoshinouchi, S.; Tanaka, Y.; Karouji, K.; Itoh, Y.; Maruyama, T.; Miyaura, C.; et al. Endosomal TLR3 Signaling in Stromal Osteoblasts Induces Prostaglandin E2–Mediated Inflammatory Periodontal Bone Resorption. J. Biol. Chem. 2022, 298, 101603. [Google Scholar] [CrossRef]
  23. Luo, G.; Li, F.; Li, X.; Wang, Z.G.; Zhang, B. TNF-α and RANKL Promote Osteoclastogenesis by Upregulating RANK via the NF-ΚB Pathway. Mol. Med. Rep. 2018, 17, 6605–6611. [Google Scholar] [CrossRef]
  24. Liao, R.; Feng, Z.; Li, W.; Liu, R.; Xu, X.; Yao, S.; Tian, J. Interleukin-1 Induces Receptor Activator of Nuclear Factor-ΚB Ligand-Independent Osteoclast Differentiation in RAW264.7 Cells. Exp. Ther. Med. 2021, 21, 640. [Google Scholar] [CrossRef]
  25. Takayanagi, H.; Kim, S.; Taniguchi, T. Signaling Crosstalk between RANKL and Interferons in Osteoclast Differentiation. Arthritis Res. Ther. 2002, 4, S227. [Google Scholar] [CrossRef] [PubMed]
  26. Ryu, J.K.; Kim, S.J.; Rah, S.H.; Kang, J.I.; Jung, H.E.; Lee, D.; Lee, H.K.; Lee, J.O.; Park, B.S.; Yoon, T.Y.; et al. Reconstruction of LPS Transfer Cascade Reveals Structural Determinants within LBP, CD14, and TLR4-MD2 for Efficient LPS Recognition and Transfer. Immunity 2017, 46, 38–50. [Google Scholar] [CrossRef]
  27. Marongiu, L.; Gornati, L.; Artuso, I.; Zanoni, I.; Granucci, F. Below the Surface: The Inner Lives of TLR4 and TLR9. J. Leukoc. Biol. 2019, 106, 147–160. [Google Scholar] [CrossRef]
  28. Miyaura, C.; Inada, M.; Matsumoto, C.; Ohshiba, T.; Uozumi, N.; Shimizu, T.; Ito, A. An Essential Role of Cytosolic Phospholipase A2α in Prostaglandin E2–Mediated Bone Resorption Associated with Inflammation. J. Exp. Med. 2003, 197, 1303–1310. [Google Scholar] [CrossRef] [PubMed]
  29. Matsumoto, C.; Ashida, N.; Yokoyama, S.; Tominari, T.; Hirata, M.; Ogawa, K.; Sugihara, M.; Yano, M.; Inada, M.; Miyaura, C. The Protective Effects of β-Cryptoxanthin on Inflammatory Bone Resorption in a Mouse Experimental Model of Periodontitis. Biosci. Biotechnol. Biochem. 2013, 77, 860–862. [Google Scholar] [CrossRef]
  30. Hirata, N.; Ichimaru, R.; Tominari, T.; Matsumoto, C.; Watanabe, K.; Taniguchi, K.; Hirata, M.; Ma, S.; Suzuki, K.; Grundler, F.M.W.; et al. Beta-Cryptoxanthin Inhibits Lipopolysaccharide-Induced Osteoclast Differentiation and Bone Resorption via the Suppression of Inhibitor of NF-ΚB Kinase Activity. Nutrients 2019, 11, 368. [Google Scholar] [CrossRef]
  31. Takeda, H.; Tominari, T.; Ichimaru, R.; Matsumoto, C.; Watanabe, K.; Hirata, M.; Inada, M.; Miyaura, C. Lutein, a Carotenoid, Inhibits Lipopolysaccharide-Induced Alveolar Bone Loss Associated with Inflammation in a Mouse Model of Periodontitis. Curr. Top. Biochem. Res. 2016, 17, 71–76. [Google Scholar]
  32. Tominari, T.; Matsumoto, C.; Watanabe, K.; Hirata, M.; Grundler, F.M.W.; Miyaura, C.; Inada, M. Epigallocatechin Gallate (EGCG) Suppresses Lipopolysaccharide-induced Inflammatory Bone Resorption, and Protects against Alveolar Bone Loss in Mice. FEBS Open Bio 2015, 5, 522–527. [Google Scholar] [CrossRef]
  33. Tominari, T.; Ichimaru, R.; Yoshinouchi, S.; Matsumoto, C.; Watanabe, K.; Hirata, M.; Grundler, F.M.W.; Inada, M.; Miyaura, C. Effects of O-methylated (−)-epigallocatechin Gallate (EGCG) on LPS-induced Osteoclastogenesis, Bone Resorption, and Alveolar Bone Loss in Mice. FEBS Open Bio 2017, 7, 1972–1981. [Google Scholar] [CrossRef]
  34. Tominari, T.; Hirata, M.; Matsumoto, C.; Inada, M.; Miyaura, C. Polymethoxy Flavonoids, Nobiletin and Tangeretin, Prevent Lipopolysaccharide-Induced Inflammatory Bone Loss in an Experimental Model for Periodontitis. J. Pharmacol. Sci. 2012, 119, 390–394. [Google Scholar] [CrossRef] [PubMed]
  35. Matsumoto, S.; Tominari, T.; Matsumoto, C.; Yoshinouchi, S.; Ichimaru, R.; Watanabe, K.; Hirata, M.; Grundler, F.M.W.; Miyaura, C.; Inada, M. Effects of Polymethoxyflavonoids on Bone Loss Induced by Estrogen Deficiency and by LPS-Dependent Inflammation in Mice. Pharmaceuticals 2018, 11, 7. [Google Scholar] [CrossRef] [PubMed]
  36. Islam, S.; Hassan, F.; Tumurkhuu, G.; Dagvadorj, J.; Koide, N.; Naiki, Y.; Mori, I.; Yoshida, T.; Yokochi, T. Bacterial Lipopolysaccharide Induces Osteoclast Formation in RAW 264.7 Macrophage Cells. Biochem. Biophys. Res. Commun. 2007, 360, 346–351. [Google Scholar] [CrossRef] [PubMed]
  37. Hou, G.Q.; Guo, C.; Song, G.H.; Fang, N.; Fan, W.J.; Chen, X.D.; Yuan, L.; Wang, Z.Q. Lipopolysaccharide (LPS) Promotes Osteoclast Differentiation and Activation by Enhancing the MAPK Pathway and COX-2 Expression in RAW264.7 Cells. Int. J. Mol. Med. 2013, 32, 503–510. [Google Scholar] [CrossRef]
  38. AlQranei, M.S.; Senbanjo, L.T.; Aljohani, H.; Hamza, T.; Chellaiah, M.A. Lipopolysaccharide- TLR-4 Axis Regulates Osteoclastogenesis Independent of RANKL/RANK Signaling. BMC Immunol. 2021, 22, 23. [Google Scholar] [CrossRef]
  39. Zou, W.; Bar-Shavit, Z. Dual Modulation of Osteoclast Differentiation by Lipopolysaccharide. J. Bone Miner. Res. 2002, 17, 1211–1218. [Google Scholar] [CrossRef]
  40. Takami, M.; Kim, N.; Rho, J.; Choi, Y. Stimulation by Toll-Like Receptors Inhibits Osteoclast Differentiation. J. Immunol. 2002, 169, 1516–1523. [Google Scholar] [CrossRef]
  41. Itoh, K.; Udagawa, N.; Kobayashi, K.; Suda, K.; Li, X.; Takami, M.; Okahashi, N.; Nishihara, T.; Takahashi, N. Lipopolysaccharide Promotes the Survival of Osteoclasts Via Toll-Like Receptor 4, but Cytokine Production of Osteoclasts in Response to Lipopolysaccharide Is Different from That of Macrophages. J. Immunol. 2003, 170, 3688–3695. [Google Scholar] [CrossRef] [PubMed]
  42. Zhou, Z.; Han, J.; Xi, C.; Xie, J.; Feng, X.; Wang, C.; Mei, L.; Xiong, W. HMGB1 Regulates RANKL-Induced Osteoclastogenesis in a Manner Dependent on RAGE1. J. Bone Miner. Res. 2009, 23, 1084–1096. [Google Scholar] [CrossRef] [PubMed]
  43. Nishida, M.; Saegusa, J.; Tanaka, S.; Morinobu, A. S100A12 Facilitates Osteoclast Differentiation from Human Monocytes. PLoS ONE 2018, 13, e0204140. [Google Scholar] [CrossRef] [PubMed]
  44. Sakamoto, Y.; Okui, T.; Yoneda, T.; Ryumon, S.; Nakamura, T.; Kawai, H.; Kunisada, Y.; Ibaragi, S.; Masui, M.; Ono, K.; et al. High-Mobility Group Box 1 Induces Bone Destruction Associated with Advanced Oral Squamous Cancer via RAGE and TLR4. Biochem. Biophys. Res. Commun. 2020, 531, 422–430. [Google Scholar] [CrossRef]
  45. Das, N.; Dewan, V.; Grace, P.M.; Gunn, R.J.; Tamura, R.; Tzarum, N.; Watkins, L.R.; Wilson, I.A.; Yin, H. HMGB1 Activates Proinflammatory Signaling via TLR5 Leading to Allodynia. Cell Rep. 2016, 17, 1128–1140. [Google Scholar] [CrossRef]
  46. Buwitt-Beckmann, U.; Heine, H.; Wiesmüller, K.-H.; Jung, G.; Brock, R.; Akira, S.; Ulmer, A.J. TLR1- and TLR6-Independent Recognition of Bacterial Lipopeptides. J. Biol. Chem. 2006, 281, 9049–9057. [Google Scholar] [CrossRef] [PubMed]
  47. Yano, A.; Suzuki, K.; Yamamoto, M.; Yamada, S. Pam3CSK4, a TLR2 Agonist, Induces Osteoclastogenesis in RAW 264.7 Cells. Dent. Med. Res. 2012, 32, 181–188. [Google Scholar] [CrossRef]
  48. Dou, C.; Zhen, G.; Dan, Y.; Wan, M.; Limjunyawong, N.; Cao, X. Sialylation of TLR2 Initiates Osteoclast Fusion. Bone Res. 2022, 10, 24. [Google Scholar] [CrossRef]
  49. Ha, H.; Lee, J.H.; Kim, H.N.; Kwak, H.B.; Kim, H.M.; Lee, S.E.; Rhee, J.H.; Kim, H.H.; Lee, Z.H. Stimulation by TLR5 Modulates Osteoclast Differentiation through STAT1/IFN-β. J. Immunol. 2008, 180, 1382–1389. [Google Scholar] [CrossRef]
  50. Kassem, A.; Henning, P.; Kindlund, B.; Lindholm, C.; Lerner, U.H. TLR5, a Novel Mediator of Innate Immunity-induced Osteoclastogenesis and Bone Loss. FASEB J. 2015, 29, 4449–4460. [Google Scholar] [CrossRef]
  51. Chamberlain, N.D.; Vila, O.M.; Volin, M.V.; Volkov, S.; Pope, R.M.; Swedler, W.; Mandelin, A.M.; Shahrara, S. TLR5, a Novel and Unidentified Inflammatory Mediator in Rheumatoid Arthritis That Correlates with Disease Activity Score and Joint TNF-α Levels. J. Immunol. 2012, 189, 475–483. [Google Scholar] [CrossRef]
  52. Kim, S.; Chen, Z.; Chamberlain, N.D.; Essani, A.B.; Volin, M.V.; Amin, M.A.; Volkov, S.; Gravallese, E.M.; Arami, S.; Swedler, W.; et al. Ligation of TLR5 Promotes Myeloid Cell Infiltration and Differentiation into Mature Osteoclasts in Rheumatoid Arthritis and Experimental Arthritis. J. Immunol. 2014, 193, 3902–3913. [Google Scholar] [CrossRef]
  53. Demaria, O.; Pagni, P.P.; Traub, S.; de Gassart, A.; Branzk, N.; Murphy, A.J.; Valenzuela, D.M.; Yancopoulos, G.D.; Flavell, R.A.; Alexopoulou, L. TLR8 Deficiency Leads to Autoimmunity in Mice. J. Clin. Investig. 2010, 120, 3651–3662. [Google Scholar] [CrossRef]
  54. Kim, K.W.; Cho, M.L.; Oh, H.J.; Kim, H.R.; Kang, C.M.; Heo, Y.M.; Lee, S.H.; Kim, H.Y. TLR-3 Enhances Osteoclastogenesis through Upregulation of RANKL Expression from Fibroblast-like Synoviocytes in Patients with Rheumatoid Arthritis. Immunol. Lett. 2009, 124, 9–17. [Google Scholar] [CrossRef] [PubMed]
  55. Karikó, K.; Ni, H.; Capodici, J.; Lamphier, M.; Weissman, D. MRNA Is an Endogenous Ligand for Toll-like Receptor 3. J. Biol. Chem. 2004, 279, 12542–12550. [Google Scholar] [CrossRef] [PubMed]
  56. Cavassani, K.A.; Ishii, M.; Wen, H.; Schaller, M.A.; Lincoln, P.M.; Lukacs, N.W.; Hogaboam, C.M.; Kunkel, S.L. TLR3 Is an Endogenous Sensor of Tissue Necrosis during Acute Inflammatory Events. J. Exp. Med. 2008, 205, 2609–2621. [Google Scholar] [CrossRef]
  57. Lee, J.; Shim, Y.; Seo, W.; Kim, M.; Choi, W.; Kim, H.; Kim, Y.E.; Yang, K.; Ryu, T.; Jeong, J.M.; et al. Mitochondrial Double-Stranded RNA in Exosome Promotes Interleukin-17 Production Through Toll-Like Receptor 3 in Alcohol-associated Liver Injury. Hepatology 2020, 72, 609–625. [Google Scholar] [CrossRef] [PubMed]
  58. Miyamoto, A.; Takami, M.; Matsumoto, A.; Mochizuki, A.; Yamada, T.; Tachi, K.; Shibuya, I.; Nakamachi, T.; Shioda, S.; Baba, K.; et al. R848, a Toll-like Receptor 7 Agonist, Inhibits Osteoclast Differentiation but Not Survival or Bone-Resorbing Function of Mature Osteoclasts. Cytotechnology 2012, 64, 331–339. [Google Scholar] [CrossRef]
  59. Suzuki, H.; Mochizuki, A.; Yoshimura, K.; Miyamoto, Y.; Kaneko, K.; Inoue, T.; Chikazu, D.; Takami, M.; Kamijo, R. Bropirimine Inhibits Osteoclast Differentiation through Production of Interferon-β. Biochem. Biophys. Res. Commun. 2015, 467, 146–151. [Google Scholar] [CrossRef]
  60. Alzabin, S.; Kong, P.; Medghalchi, M.; Palfreeman, A.; Williams, R.; Sacre, S. Investigation of the Role of Endosomal Toll-like Receptors in Murine Collagen-Induced Arthritis Reveals a Potential Role for TLR7 in Disease Maintenance. Arthritis Res. Ther. 2012, 14, R142. [Google Scholar] [CrossRef]
  61. Kim, K.W.; Kim, B.M.; Won, J.Y.; Lee, K.A.; Kim, H.R.; Lee, S.H. Toll-like Receptor 7 Regulates Osteoclastogenesis in Rheumatoid Arthritis. J. Biochem. 2019, 166, 259–270. [Google Scholar] [CrossRef] [PubMed]
  62. Hegewald, A.B.; Breitwieser, K.; Ottinger, S.M.; Mobarrez, F.; Korotkova, M.; Rethi, B.; Jakobsson, P.-J.; Catrina, A.I.; Wähämaa, H.; Saul, M.J. Extracellular MiR-574-5p Induces Osteoclast Differentiation via TLR 7/8 in Rheumatoid Arthritis. Front. Immunol. 2020, 11, 585282. [Google Scholar] [CrossRef]
  63. Amcheslavsky, A.; Bar-Shavit, Z. Toll-Like Receptor 9 Ligand Blocks Osteoclast Differentiation Through Induction of Phosphatase. J. Bone Miner. Res. 2007, 22, 1301–1310. [Google Scholar] [CrossRef]
  64. Zou, W.; Schwartz, H.; Endres, S.; Hartmann, G.; Bar-Shavit, Z. CpG Oligonucleotides: Novel Regulators of Osteoclast Differentiation. FASEB J. 2002, 16, 274–282. [Google Scholar] [CrossRef] [PubMed]
  65. Ding, P.; Tan, Q.; Wei, Z.; Chen, Q.; Wang, C.; Qi, L.; Wen, L.; Zhang, C.; Yao, C. Toll-like Receptor 9 Deficiency Induces Osteoclastic Bone Loss via Gut Microbiota-Associated Systemic Chronic Inflammation. Bone Res. 2022, 10, 42. [Google Scholar] [CrossRef] [PubMed]
  66. Kim, P.D.; Xia-Juan, X.; Crump, K.E.; Abe, T.; Hajishengallis, G.; Sahingur, S.E. Toll-Like Receptor 9-Mediated Inflammation Triggers Alveolar Bone Loss in Experimental Murine Periodontitis. Infect. Immun. 2015, 83, 2992–3002. [Google Scholar] [CrossRef]
  67. Vanaja, S.K.; Russo, A.J.; Behl, B.; Banerjee, I.; Yankova, M.; Deshmukh, S.D.; Rathinam, V.A.K. Bacterial Outer Membrane Vesicles Mediate Cytosolic Localization of LPS and Caspase-11 Activation. Cell 2016, 165, 1106–1119. [Google Scholar] [CrossRef]
  68. Hagar, J.A.; Powell, D.A.; Aachoui, Y.; Ernst, R.K.; Miao, E.A. Cytoplasmic LPS Activates Caspase-11: Implications in TLR4-Independent Endotoxic Shock. Science 2013, 341, 1250–1253. [Google Scholar] [CrossRef]
  69. Miao, E.A.; Mao, D.P.; Yudkovsky, N.; Bonneau, R.; Lorang, C.G.; Warren, S.E.; Leaf, I.A.; Aderem, A. Innate Immune Detection of the Type III Secretion Apparatus through the NLRC4 Inflammasome. Proc. Natl. Acad. Sci. USA 2010, 107, 3076–3080. [Google Scholar] [CrossRef]
  70. Zhao, Y.; Yang, J.; Shi, J.; Gong, Y.N.; Lu, Q.; Xu, H.; Liu, L.; Shao, F. The NLRC4 Inflammasome Receptors for Bacterial Flagellin and Type III Secretion Apparatus. Nature 2011, 477, 596–600. [Google Scholar] [CrossRef]
  71. Alippe, Y.; Wang, C.; Ricci, B.; Xiao, J.; Qu, C.; Zou, W.; Novack, D.V.; Abu-Amer, Y.; Civitelli, R.; Mbalaviele, G. Bone Matrix Components Activate the NLRP3 Inflammasome and Promote Osteoclast Differentiation. Sci. Rep. 2017, 7, 6630. [Google Scholar] [CrossRef]
  72. Alippe, Y.; Kress, D.; Ricci, B.; Sun, K.; Yang, T.; Wang, C.; Xiao, J.; Abu-Amer, Y.; Mbalaviele, G. Actions of the NLRP3 and NLRC4 Inflammasomes Overlap in Bone Resorption. FASEB J. 2021, 35, e21837. [Google Scholar] [CrossRef] [PubMed]
  73. Reikine, S.; Nguyen, J.B.; Modis, Y. Pattern Recognition and Signaling Mechanisms of RIG-I and MDA5. Front. Immunol. 2014, 5, 342. [Google Scholar] [CrossRef]
  74. Wang, X.; Lin, M.; Zhu, L.; Ye, Z. GAS-STING: A Classical DNA Recognition Pathways to Tumor Therapy. Front. Immunol. 2023, 14, 1200245. [Google Scholar] [CrossRef] [PubMed]
  75. MacLauchlan, S.; Kushwaha, P.; Tai, A.; Chen, S.; Manning, C.; Swarnkar, G.; Abu-Amer, Y.; Fitzgerald, K.A.; Sharma, S.; Gravallese, E.M. STING-Dependent Interferon Signatures Restrict Osteoclast Differentiation and Bone Loss in Mice. Proc. Natl. Acad. Sci. USA 2023, 120, e2210409120. [Google Scholar] [CrossRef] [PubMed]
  76. Li, W.; Zhang, Z.; Wang, Z.M. Differential immune cell infiltrations between healthy periodontal and chronic periodontitis tissues. BMC Oral Health 2020, 20, 293. [Google Scholar] [CrossRef] [PubMed]
  77. Chakravarti, A.; Raquil, M.A.; Tessier, P.; Poubelle, P.E. Surface RANKL of Toll-like receptor 4-stimulated human neutrophils activates osteoclastic bone resorption. Blood 2009, 114, 1633–1644. [Google Scholar] [CrossRef]
  78. Poli, C.; Martin, J.C.; Braudeau, C.; Bériou, G.; Hémont, C.; Charrier, C.; Guérin, S.; Heslan, M.; Josien, R. Receptor activating NF-κB ligand (RANKL) is a constitutive intracellular protein in resting human basophils and is strongly induced on their surface by interleukin 3. Immunobiology 2015, 220, 692–700. [Google Scholar] [CrossRef]
  79. Loser, K.; Mehling, A.; Loeser, S.; Apelt, J.; Kuhn, A.; Grabbe, S.; Schwarz, T.; Penninger, J.M.; Beissert, S. Epidermal RANKL controls regulatory T-cell numbers via activation of dendritic cells. Nat. Med. 2006, 12, 1372–1379. [Google Scholar] [CrossRef]
  80. Schmiedel, B.J.; Nuebling, T.; Steinbacher, J.; Malinovska, A.; Wende, C.M.; Azuma, M.; Schneider, P.; Grosse-Hovest, L.; Salih, H.R. Receptor activator for NF-κB ligand in acute myeloid leukemia: Expression, function, and modulation of NK cell immunosurveillance. J. Immunol. 2013, 190, 821–831. [Google Scholar] [CrossRef]
  81. Hienz, S.A.; Paliwal, S.; Ivanovski, S. Mechanisms of Bone Resorption in Periodontitis. J. Immunol. Res. 2015, 2015, 615486. [Google Scholar] [CrossRef]
  82. Ando, Y.; Tsukasaki, M.; Huynh, N.C.; Zang, S.; Yan, M.; Muro, R.; Nakamura, K.; Komagamine, M.; Komatsu, N.; Okamoto, K.; et al. The neutrophil-osteogenic cell axis promotes bone destruction in periodontitis. Int. J. Oral Sci. 2024, 16, 18. [Google Scholar] [CrossRef] [PubMed]
  83. Makkawi, H.; Hoch, S.; Burns, E.; Hosur, K.; Hajishengallis, G.; Kirschning, C.J.; Nussbaum, G. Porphyromonas gingivalis Stimulates TLR2-PI3K Signaling to Escape Immune Clearance and Induce Bone Resorption Independently of MyD88. Front. Cell Infect. Microbiol. 2017, 7, 359. [Google Scholar] [CrossRef] [PubMed]
  84. Guilherme Neto, J.L.; Rodrigues Venturini, L.G.; Schneider, A.H.; Taira, T.M.; Duffles Rodrigues, L.F.; Veras, F.P.; Oliveira, S.R.; da Silva, T.A.; Cunha, F.Q.; Fukada, S.Y. Neutrophil Extracellular Traps Aggravate Apical Periodontitis by Stimulating Osteoclast Formation. J. Endod. 2023, 49, 1514–1521. [Google Scholar] [CrossRef]
  85. Kim, T.S.; Silva, L.M.; Theofilou, V.I.; Greenwell-Wild, T.; Li, L.; Williams, D.W.; Ikeuchi, T.; Brenchley, L. NIDCD/NIDCR Genomics and Computational Biology Core; Bugge, T.H.; et al. Neutrophil extracellular traps and extracellular histones potentiate IL-17 inflammation in periodontitis. J. Exp. Med. 2023, 220, e20221751. [Google Scholar] [CrossRef] [PubMed]
  86. Chen, T.; Li, Y.; Sun, R.; Hu, H.; Liu, Y.; Herrmann, M.; Zhao, Y.; Muñoz, L.E. Receptor-Mediated NETosis on Neutrophils. Front. Immunol. 2021, 12, 775267. [Google Scholar] [CrossRef]
  87. Han, Y.K.; Jin, Y.; Miao, Y.B.; Shi, T.; Lin, X.P. CD8+ Foxp3+ T Cells Affect Alveolar Bone Homeostasis via Modulating Tregs/Th17 During Induced Periodontitis: An Adoptive Transfer Experiment. Inflammation 2018, 41, 1791–1803. [Google Scholar] [CrossRef]
  88. Li, S.; Su, L.; Luan, Q.; Liu, G.; Zeng, W.; Yu, X. Regulatory B cells induced by interleukin-35 inhibit inflammation and alveolar bone resorption in ligature-induced periodontitis. J. Periodontol. 2023, 94, 1376–1388. [Google Scholar] [CrossRef]
  89. Luo, C.Y.; Wang, L.; Sun, C.; Li, D.J. Estrogen enhances the functions of CD4(+)CD25(+)Foxp3(+) regulatory T cells that suppress osteoclast differentiation and bone resorption in vitro. Cell Mol. Immunol. 2011, 8, 50–58. [Google Scholar] [CrossRef]
Biology 13 00692 g001
Figure 1. TLR signaling pathways. TLR2/1, TLR2/6, and TLR4 transduce the TIRAP/MyD88-dependent pathway to activate NF-κB and AP-1 transcription, which induces the production of pro-inflammatory cytokines. TLR5, TLR7/8, and TLR9 activate the MyD88-dependent pathway without the TIRAP adaptor protein. In contrast, TLR3 and endocytosed TLR4 activate a TRIF-dependent pathway to activate both NF-κB and IRFs. Endocytosed TLR4 requires a TRAM adaptor protein. TLR7/8 and TLR9 also transduce the IRF7 and NF-κB pathways.
Figure 1. TLR signaling pathways. TLR2/1, TLR2/6, and TLR4 transduce the TIRAP/MyD88-dependent pathway to activate NF-κB and AP-1 transcription, which induces the production of pro-inflammatory cytokines. TLR5, TLR7/8, and TLR9 activate the MyD88-dependent pathway without the TIRAP adaptor protein. In contrast, TLR3 and endocytosed TLR4 activate a TRIF-dependent pathway to activate both NF-κB and IRFs. Endocytosed TLR4 requires a TRAM adaptor protein. TLR7/8 and TLR9 also transduce the IRF7 and NF-κB pathways.
Biology 13 00692 g001
Biology 13 00692 g002
Figure 2. Roles of TLR signaling in osteoclast differentiation. In osteoblasts, TLR2/1, TLR2/6, TLR3, and TLR4 signaling activates the NF-κB pathway, leading to PGE2 production mediated by COX2 and mPGES-1. PGE2 activates EP4 signaling in an autocrine/paracrine manner, followed by RANKL expression. In addition, these signaling pathways directly activate the osteoclast function and survival via the NF-κB pathway. Both indirect and direct effects of TLR signaling induce osteoclastic bone resorption.
Figure 2. Roles of TLR signaling in osteoclast differentiation. In osteoblasts, TLR2/1, TLR2/6, TLR3, and TLR4 signaling activates the NF-κB pathway, leading to PGE2 production mediated by COX2 and mPGES-1. PGE2 activates EP4 signaling in an autocrine/paracrine manner, followed by RANKL expression. In addition, these signaling pathways directly activate the osteoclast function and survival via the NF-κB pathway. Both indirect and direct effects of TLR signaling induce osteoclastic bone resorption.
Biology 13 00692 g002
Biology 13 00692 g003
Figure 3. Schematic illustration of inflammatory bone resorption induced by TLR ligands. In inflammatory tissues, such as periodontal tissues infected with bacteria and arthritic joints, bacterial ligands derived from infected bacteria and endogenous ligands derived from host cells, such as immune cells and stromal cells, are recognized by the cell surface and endosomal/lysosomal TLRs and cytosolic sensors (RIG-I/MDA5, cGAS-STING, and inflammasomes), stimulating the production of inflammatory cytokines and PGE2. These factors induce osteoclast differentiation and bone resorption, which are associated with inflammation.
Figure 3. Schematic illustration of inflammatory bone resorption induced by TLR ligands. In inflammatory tissues, such as periodontal tissues infected with bacteria and arthritic joints, bacterial ligands derived from infected bacteria and endogenous ligands derived from host cells, such as immune cells and stromal cells, are recognized by the cell surface and endosomal/lysosomal TLRs and cytosolic sensors (RIG-I/MDA5, cGAS-STING, and inflammasomes), stimulating the production of inflammatory cytokines and PGE2. These factors induce osteoclast differentiation and bone resorption, which are associated with inflammation.
Biology 13 00692 g003
Table 1. TLR ligands.
Table 1. TLR ligands.
TLRLocationExogenous Ligands
(MAMPs)		Endogenous Ligands
(DAMPs)
TLR2/1 and/or TLR2/6Cell surface
(Cell membrane)		Peptide glycanAmyloid,
HSPs,
HMGB1,
Hyaluronan
TLR2/1Cell surface
(Cell membrane)		Triacylated lipopeptide-
TLR2/6Cell surface
(Cell membrane)		Diacylated lipopeptide,
LTA,
Fungal zymosan		-
TLR3Endosome/lysosomedsRNAsnRNA,
Mitochondrial dsRNA,
RNA from dead cells
TLR4Cell surface
(Cell membrane)		LPS,
Virus envelope protein		Oxidized LDL,
AGE-LDL,
HSPs,
HMGB1,
Hyaluronan,
S100 proteins,
Fibrinogen,
Anti-MPs
TLR5Cell surface
(Cell membrane)		Flagellin
Fungal zymosan		Lectin,
HSPs
TLR7Endosome/lysosomessRNARNA from dead cells
Anti-MPs
TLR8Endosome/lysosomessRNARNA from dead cells
Anti-MPs
TLR9Endosome/lysosomeUnmethylated CpG DNAHMGB1,
Mitochondria DNA,
Anti-MPs
TLR10Endosome/lysosome--
TLR11Cell surface
(Cell membrane)		Profilin-like protein-
TLR12Endosome/lysosomeProfilin-
TLR13Endosome/lysosome23S rRNA-
HSP: Heat-shock protein, HMGB1: high-mobility group box 1, LTA: lipoteichoic acid, dsRNA: double-stranded RNA, snRNA: small nuclear RNA, LPS: lipopolysaccharide, LDL: low-density lipoprotein, AGE: advanced glycation end product, Anti-MP: antimicrobial peptide, ssRNA: single-strand RNA.
Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Share and Cite

MDPI and ACS Style

Tominari, T.; Matsumoto, C.; Tanaka, Y.; Shimizu, K.; Takatoya, M.; Sugasaki, M.; Karouji, K.; Kasuga, U.; Miyaura, C.; Miyata, S.; et al. Roles of Toll-like Receptor Signaling in Inflammatory Bone Resorption. Biology 2024, 13, 692. https://doi.org/10.3390/biology13090692

AMA Style

Tominari T, Matsumoto C, Tanaka Y, Shimizu K, Takatoya M, Sugasaki M, Karouji K, Kasuga U, Miyaura C, Miyata S, et al. Roles of Toll-like Receptor Signaling in Inflammatory Bone Resorption. Biology. 2024; 13(9):692. https://doi.org/10.3390/biology13090692

Chicago/Turabian Style

Tominari, Tsukasa, Chiho Matsumoto, Yuki Tanaka, Kensuke Shimizu, Masaru Takatoya, Moe Sugasaki, Kento Karouji, Urara Kasuga, Chisato Miyaura, Shinji Miyata, and et al. 2024. "Roles of Toll-like Receptor Signaling in Inflammatory Bone Resorption" Biology 13, no. 9: 692. https://doi.org/10.3390/biology13090692

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop