Which Zebrafish Strains Are More Suitable to Perform Behavioral Studies? A Comprehensive Comparison by Phenomic Approach
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Strains Used in This Study
2.2. Zebrafish Behavioral Tests and Ethics
2.3. Video-Tracking and Statistical Data Analysis
2.4. Principal Component Analysis (PCA), Heatmap, and Hierarchical Clustering Analysis
3. Results
3.1. Absolute, Golden, and Pet Store-Purchased (PET) Zebrafish Displayed Different Behaviors in the Novel Tank Test
3.2. Different Level of Aggressiveness from AB Zebrafish Was Displayed by Absolute, Tübingen Long Fin (TL), Golden, and Wild Indian Karyotype (WIK) Zebrafish
3.3. Absolute, Tübingen Long Fin (TL), and Wild Indian Karyotype (WIK) Zebrafish Displayed Less Predator Avoidance Behaviors than the AB Zebrafish in the Predator Avoidance Test
3.4. Absolute, Pet Store-Purchased (PET), and Wild Indian Karyotype (WIK) Zebrafish Displayed Less Interest to the Conspecific than the AB Zebrafish in the Social Interaction Test
3.5. Loose Shoal Was Formed by Pet Store-Purchased (PET) and Wild Indian Karyotype (WIK) Zebrafishes in the Shoaling Test
3.6. Principal Component Analysis (PCA) Analysis and Hierarchical Clustering Analysis of Several Fish Behavioral Endpoints
4. Discussions and Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Multiple Fish Behavior Tests | ||||||
---|---|---|---|---|---|---|
Fish Strains | Locomotor Activity | Exploratory Behavior | Aggressiveness | Predator Avoidance | Conspecific Social Interaction | Shoaling Size |
Absolute Zebrafish | - | ↓↓↓↓ | ↓↓ | ↓↓ | ↓↓↓ | - |
TL Zebrafish | - | - | ↓ | ↓ | - | - |
Golden Zebrafish | ↑↑ | ↓↓↓ | ↑ | - | - | - |
PET Zebrafish | ↑↑ | ↓↓↓↓↓ | - | - | ↓↓ | ↑↑ |
WIK Zebrafish | - | ↓ | ↓ | ↓↓ | ↓↓↓ | ↑ |
Puntigrus tetrazona | ↑↑ | ↑↑↑ | ↑↑ | ↓↓ | ↓↓ | ↓ |
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Audira, G.; Siregar, P.; Strungaru, S.-A.; Huang, J.-C.; Hsiao, C.-D. Which Zebrafish Strains Are More Suitable to Perform Behavioral Studies? A Comprehensive Comparison by Phenomic Approach. Biology 2020, 9, 200. https://doi.org/10.3390/biology9080200
Audira G, Siregar P, Strungaru S-A, Huang J-C, Hsiao C-D. Which Zebrafish Strains Are More Suitable to Perform Behavioral Studies? A Comprehensive Comparison by Phenomic Approach. Biology. 2020; 9(8):200. https://doi.org/10.3390/biology9080200
Chicago/Turabian StyleAudira, Gilbert, Petrus Siregar, Stefan-Adrian Strungaru, Jong-Chin Huang, and Chung-Der Hsiao. 2020. "Which Zebrafish Strains Are More Suitable to Perform Behavioral Studies? A Comprehensive Comparison by Phenomic Approach" Biology 9, no. 8: 200. https://doi.org/10.3390/biology9080200