Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion
Round 1
Reviewer 1 Report (Previous Reviewer 2)
Comments and Suggestions for AuthorsDear Authors,
I write in regard to your manuscript entitled Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion.
- the reference (12) is from 2008. Certainly, new references challenged the photoprotection properties involving clinical assays of rutin (SPF and rutin), rosmarinic acid (SPF), caffeine (another reason to use caffeine), ferulic acid etc. Please, revise and update your references in the introduction section.
- the abstract must be written in the past tense. please, revise the abstract.
- please, add "Limitation and Strenghts".
Author Response
Comment 1: the reference (12) is from 2008. Certainly, new references challenged the photoprotection properties involving clinical assays of rutin (SPF and rutin), rosmarinic acid (SPF), caffeine (another reason to use caffeine), ferulic acid etc. Please, revise and update your references in the introduction section.
Response1: New reference from year 2021 was used for the older 2008.
Comment 2: the abstract must be written in the past tense. please, revise the abstract.
Response1: Thank you for pointing this out. The abstract was revised and the English language and errors were also carefully checked and corrected throughout the manuscript.
Comment 3: please, add "Limitation and Strenghts"
Response 3: The ‘strengths and limitation’ of manuscript was added in the very last on the ‘Conclusion’ section since there is no part of this in the template of the journal.
The authors are very appreciated for all voluble comments and would like to express our thankfulness to the reviewer.
Reviewer 2 Report (Previous Reviewer 1)
Comments and Suggestions for AuthorsThe paper Nattakan Panyachariwat et all entitled Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion is revised version of manuscript.
The authors revised their manuscript and respond to all my comments. They performed additional analysis and test such as UHPLC-QTOF-MS and cytotoxic effect if E. bulbosa or viscosity. They made also corrections such as provide additional information such as INCI name, function in recipe. More information on the E. bulbosa properties and application was added in the introduction part and the conclusion was revised. I accept manuscript in the present form.
Comments on the Quality of English LanguageThe paper Nattakan Panyachariwat et all entitled Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion is revised version of manuscript.
The authors revised their manuscript and respond to all my comments. They performed additional analysis and test such as UHPLC-QTOF-MS and cytotoxic effect if E. bulbosa or viscosity. They made also corrections such as provide additional information such as INCI name, function in recipe. More information on the E. bulbosa properties and application was added in the introduction part and the conclusion was revised. I accept manuscript in the present form.
Author Response
Comments 1: The authors revised their manuscript and respond to all my comments. They performed additional analysis and test such as UHPLC-QTOF-MS and cytotoxic effect if E. bulbosa or viscosity. They made also corrections such as provide additional information such as INCI name, function in recipe. More information on the E. bulbosa properties and application was added in the introduction part and the conclusion was revised. I accept manuscript in the present form.
Response 1: The authors are very appreciated for all valuable comments and would like to express our thankfulness to the reviewer. Besides, we have carefully revised for errors and English language and corrections were made throughout the manuscript.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
Round 1
Reviewer 1 Report
Comments and Suggestions for AuthorsTo the Authors,
Title: Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion
Manuscript Summary: In this manuscript, Nattakan Panyachariwat et. al have described antioxidative potentials of Eleutherine bulbosa. In addition, the authors have also presented its utilization in topical emulsion. Although, the manuscript is well organized and properties of Eleutherine bulbosa Bulb are current topic, as a reviewer, I must point out the following:
My biggest concerns are related to methodology. To describe antioxidant potentials of extract especially when Authors used new solvents (propylene glycol, mixture of propylene glycol and ethanol) the quantitative and not only qualitative methods should be used i.e. HPLC-MS Analysis. Also cytotoxic effect of E. bulbosa should be evaluated for all analyzed extract not only for crude ethanolic extract. Especially when Authors claim that E. bulbosa had safety potentials in cosmetic preparations.
For the characteristics of the emulsion, Authors didn’t perform necessary research such as viscosity. I also cannot agree with the methodology used to perform stability tests. The minimum time period of storage sample should be 3 months (preferably 6 month) not 1 month, especially when after 1 month the DPPH inhibition activity significantly decreased. If Authors extracted E. bulbosa from emulsion why only radical scavenging activity of products was assayed?
Other concerns:
1. All ingredients for emulsion preparation should be mentioned in Materials section. Additionally in Materials section and in Table 1 (composition of emulsion product) should be INCI name used. In table 1 there is also incorrect name of Sorbitan 60. It should be Span 60 (trade name) or Sorbitane monostearate. Polysorbate 60 is soluble in water so why Authors add it to oil phase? Because article is under consideration to be published in Cosmetics, function in recipe for all ingredients (according to Cosing data base) should be also provided.
2. Please provide standard deviation for pH value.
3. Page 11 line 415 “E. bulbosa bulb concentrated extract (1.0 and 2.0 % (w/w)) was added in emulsion cream, and it rarely affected the pH and viscosity of the prepared emulsion” On which basic Authors claim that extract did not affect the viscosity since no such tests were conducted?
4. In Section 6, the authors filed a patent. What does it refer to?
5. In introduction part Authors could also presents information about cosmetic formulations (creams, powders) with E. bulbosa ethanol extract which are used to acne treatment. Also conclusions section in my opinion looks like summary not conclusions. There are a lot of repetitions of result not specific conclusions.
Reviewer 2 Report
Comments and Suggestions for AuthorsDear Authors,
I write you in regard to the manuscript Antioxidative Potentials of Eleutherine bulbosa Bulb and Its Utilization in Topical Cosmetic Emulsion.
- in introduction, to valorize the content, try to associate the sun protection property of some of the compounds found in the E. bulbosa, like rutin (in vitro and in vivo tests os sunscreens, for exemple).
- authors presented a very strategic way to describe the objectives. very interesting.
- please, described the ingredients of the formulation by chemical name or the INCI name. Try to avoid commercial name.
- Table 2 was difficult to visualize in terms of the statistical notification.
- considering the lack of stability of the active at 45C, try to present perspectives to improve the emulsion stability.
- in conclusion, it was reported that the sample was stable at 45C. However, it was not since the antioxidant property was highly damaged by the storage condition. This must be revised in the conclusion.
