Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species
Abstract
:1. Introduction
2. Materials and Methods
2.1. Collecting and Identification of Plant Materials
2.2. Preparation of Mucuna Seed Extracts
2.3. Determination of L-DOPA Content
2.4. Determination of Total Phenolic Content
2.5. Determination of Total Flavonoid Content
2.6. Gas Chromatography–Mass Spectrometry Analysis
2.7. Antimicrobial Activity Assay
2.8. Antioxidant Activity Assay
2.8.1. 1,1-Diphenyl-2-Picrylhydrazyl (DPPH) Radical Scavenging Assay
2.8.2. Ferric Reducing Antioxidant Power (FRAP) Assay
2.8.3. Lipid Peroxidation Inhibition Assay Using Linoleic Acid Thiocyanate Method
2.9. Hyaluronidase Inhibitory Assay
2.10. Collagenase Inhibitory Activity
2.11. Elastase Inhibitory Activity
2.12. Statistical Analysis
3. Results and Discussions
3.1. Identification of Plant Materials Using a Mini-Barcode, ITS2 Intergenic Region
3.2. Determination of L-DOPA and, Total Phenolic, and Flavonoid Content
3.3. Phytochemical Analysis Using GC–MS
3.4. Skin-Related Antimicrobial Activities
3.5. Antioxidant Activity Assay
3.6. Anti-Aging Properties
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Sample Codes | Expected Species | Collected Dates | Localities (District, Province) |
---|---|---|---|
MGG | M.gigantea (Willd.) DC. | 24 November 2017 | Khuan Niang, Songkhla |
MIT | M.interrupta Gagnep. | 10 June 2017 | Mae Sariang, Mae Hong Son |
MMM | M.monosperma Wight | 24 May 2017 | Mae Sot, Tak |
MPR | M. pruriens (L.) DC. | 3 September 2017 | Ban Na Doem, Surat Thani |
Sample Codes | Species of BLAST Results | Accession Number | Max Score | Percent Identity (%) |
---|---|---|---|---|
MGG | M.gigantea | LC494611 | 612 | 99.11 |
MIT | M.interrupta | HM355841 | 675 | 98.94 |
MMM | M.monosperma | AB775135 | 625 | 98.06 |
MPR | M. pruriens | KX057890 | 730 | 99.50 |
Extracts | C. acnes DMST 14916 | S. aureus TISTR 746 | S. epidermidis TISTR 2141 | C. albicans TISTR 5239 | ||||
---|---|---|---|---|---|---|---|---|
MIC (mg/mL) | MBC (mg/mL) | MIC (mg/mL) | MBC (mg/mL) | MIC (mg/mL) | MFC (mg/mL) | MIC (mg/mL) | MBC (mg/mL) | |
MGG | Nd. | Nd. | Nd. | Nd. | Nd. | Nd. | Nd. | Nd. |
MIT | Nd. | Nd. | Nd. | Nd. | Nd. | Nd. | 8.20 | 16.40 |
MMM | Nd. | Nd. | 8.20 | 16.40 | 8.20 | 16.40 | 4.10 | 8.20 |
MPR | Nd. | Nd. | 4.10 | 4.10 | Nd. | Nd. | Nd. | Nd. |
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Theansungnoen, T.; Nitthikan, N.; Wilai, M.; Chaiwut, P.; Kiattisin, K.; Intharuksa, A. Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species. Cosmetics 2022, 9, 14. https://doi.org/10.3390/cosmetics9010014
Theansungnoen T, Nitthikan N, Wilai M, Chaiwut P, Kiattisin K, Intharuksa A. Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species. Cosmetics. 2022; 9(1):14. https://doi.org/10.3390/cosmetics9010014
Chicago/Turabian StyleTheansungnoen, Tinnakorn, Nichcha Nitthikan, Mayuramas Wilai, Phanuphong Chaiwut, Kanokwan Kiattisin, and Aekkhaluck Intharuksa. 2022. "Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species" Cosmetics 9, no. 1: 14. https://doi.org/10.3390/cosmetics9010014
APA StyleTheansungnoen, T., Nitthikan, N., Wilai, M., Chaiwut, P., Kiattisin, K., & Intharuksa, A. (2022). Phytochemical Analysis and Antioxidant, Antimicrobial, and Antiaging Activities of Ethanolic Seed Extracts of Four Mucuna Species. Cosmetics, 9(1), 14. https://doi.org/10.3390/cosmetics9010014