Compounds in Indonesian Ginger Rhizome Extracts and Their Potential for Anti-Skin Aging Based on Molecular Docking
, Ayu Rahmania Lestari 1, Wulan Tri Wahyuni 1,2 and Setyanto Tri Wahyudi 2,3
Round 1
Reviewer 1 Report
November 3, 2022
This paper described the compounds identification from Indonesian ginger rhizome extract and molecular docking study of octinoxate from the extract. The topic is interesting but some major points are to be cleared before acceptance of the paper.
1. The authors focus on skin anti-aging among many different facets of anti-aging. So the title, abstract, introduction, and discussion should be rewritten in this aspect. Skin anti-aging should be stressed out.
2. The title should be changed including skin anti-aging compounds or something like that. And molecular docking study were done on vitamin C and octinoxate, so it should be mentioned in the title and abstract.
3. Many editorial errors. In line 12, Indonesian gingers, ----, are. In line 17, PCA should be written in full name.
4. Introduction started with skin, but it should be started with anti-aging and narrowed the scope to skin anti-aging.
5. Yin and yang of molecular docking study should be mentioned.
6. In line 90, GPS location of the growth and harvest area should be described as well as harvest conditions.
7. In line 94, extraction temp, time and apparatus should be mentioned.
8. All the machines used in this study have to include the model name, company name, city, and country names.
9. In line 121, appropriate website should be included to reproduce the receptor structure.
10. In line 170, there are many unknown peaks in chromatogram. Some remarks should be included in Results and Discussion section about that.
11. Fig. 4, 5, 6, and 7 should be merged in one summary figure.
12. In References, italic fonts check in Ref 12, and recheck the bib info in Ref 18.
Author Response
Thank you so much for your evaluation of our manuscript. We appreciate you and editor for providing very constructive suggestions and comments and offering the opportunity to us for making a revision. We have made careful revisions according to the reviewer’s and editor’s comments, and the point-to-point responses have been stated below. The changes in the manuscript were highlighted in red, seeing the file of “Revised Manuscript”.
Author Response File: 
Author Response.pdf
Reviewer 2 Report
Research indicates that skin aging processes are caused by reactive oxygen species (ROS) and advanced glycation end products (AGEs). Ginger, a well-known natural product, has been shown to possess antioxidant, anti-inflammatory, anti-cancer and antibacterial properties. In this study, the authors investigated ethanolic extracts of three types of Indonesian ginger rhizomes (GG, EE, MM) to identify compounds with the most potent anti-aging effects. The authors used Liquid Chromatography-Mass Spectrometry / Mass Spectrometry (LC-MS / MS). The statistical evaluation of the results was performed using the ANOVA method. Molecular docking simulations were performed with Autodock Vina. The molecular docking results were analyzed to obtain the test ligand with the most negative affinity energy. The enzymes also used in the molecular docking study were collagenase, hyaluronidase, elastase and tyrosinase.
The authors demonstrated that EE ginger rhizome extract had potential as an anti-aging ingredient compared to other Indonesian ginger rhizome extracts due to its high octinoxate content.
The analysis of the results and their discussion is carried out by the authors very carefully and does not raise any objections.
However, I recommend the authors to include a short chapter in the manuscript justifying the use of their extract in cosmetic products.
I recommend minor revision.
Author Response
Dear Reviewers
Thank you so much for your evaluation of our manuscript. We appreciate you and editor for providing very constructive suggestions and comments and offering the opportunity to us for making a revision. We have made careful revisions according to the reviewer’s and editor’s comments, and the point-to-point responses have been stated below. The changes in the manuscript were highlighted in red, seeing the file of “Revised Manuscript”.
As your suggestion, we had added a brief explanation about ginger extract in skin care and cosmetics industry on the Introduction part (line 70 – 87).
Thank you so much.
Author Response File: Author Response.pdf
Reviewer 3 Report
The paper is interesting and innovative. The English language and style required some changes
Line 33: “Among these three problems, skin aging is a sure thing to happen to live things” - reforming this sentence
Author Response
Dear Reviewers
Thank you so much for your evaluation of our manuscript. We appreciate you and editor for providing very constructive suggestions and comments and offering the opportunity to us for making a revision. We have made careful revisions according to the reviewer’s and editor’s comments, and the point-to-point responses have been stated below. The changes in the manuscript were highlighted in red, seeing the file of “Revised Manuscript”.
For line 33: We had deleted that sentence.
Author Response File: Author Response.pdf
