Metabolite Profile of Athrixia phylicoides DC. (Bush Tea) and Determination of Inhibitory Mechanism against Tyrosinase Enzyme from Mushroom
, Maanea Lonia Ramphinwa 2, Godwin Richard Ainamensa Mchau 2 and Fhatuwani Nixwell Mudau 3
Round 1
Reviewer 1 Report
The manuscript on Metabolite profile of Athrixia phylicoides DC. (Bush tea) and determination of inhibitory mechanism against mushroom tyrosinase is well written and sound. Methods used are appropriate and results are well discussed.
Methods: Why was methanol the only solvent used for extraction? Further study should include the use of more solvents for extraction.
Do the authors intend to check and identify the exact metabolite responsible for the activity of the plant under study? It would be valuable to do this.
Author Response
Comment: Methods: Why was methanol the only solvent used for extraction? Further study should include the use of more solvents for extraction.
Response: Literature (Dhawan and Gupta et al., 2017) has reported on methanol as an effective extraction solvent for various phytochemicals hence the solvent was chosen for the present study. Various solvents of varying polarities are been investigated for their inhibitory effect on the enzyme.
Comment: Do the authors intend to check and identify the exact metabolite responsible for the activity of the plant under study? It would be valuable to do this.
Response: The tyrosinase-based TLC autography was an effective assay for highlighting those metabolites and hence the on-going studies are on isolating and purifying the active metabolite and incorporation in a cosmeceutical product.
Reviewer 2 Report
The manuscript entitled " Metabolite profile of Athrixia phylicoides DC. (Bush tea) and determination of inhibitory mechanism against mushroom tyrosinase" mainly focuses on use of plant extract enzyme inhibition and cytotoxic effects. From the contents of the manuscript to estimate, this research is good in the qualitative and quantitative analysis. In my judgement, it needs to be revised for publication in this journal.
Title: is tyrosinase is a mushroom? “mechanism against mushroom tyrosinase” could be revise as “against an enzyme tyrosinase from mushroom”
Abstract
Abstract is well written. However, results should be further specified specifically in quantitative values.
Also add which mushroom was used for extraction of tyrosinase
Introduction
Intro is very short; it needs modification for example the author should provide general background of the topic.
Intro should be start from the potential of medicinal plants in against various disease and specifically against skin diseases. The following studies could be helpful and cited.
https://doi.org/10.3390/molecules27196728, https://doi.org/10.1016/j.chnaes.2021.03.009,
Line 55 also add other references as the author used word researchers.
Line 66 also add other references as the author used word studies.
The author must discuss recent advances of the use of botanicals and plant extracts in enzyme inhibition.
Why specific enzyme tyrosinase inhibition was used in this study provide the reason in introduction. What is its link with skin imperfection?
Figure 1 is not very clear.
In conclusion present future recommendations.
Author Response
Comment: Title: is tyrosinase is a mushroom? “mechanism against mushroom tyrosinase” could be revise as “against an enzyme tyrosinase from mushroom”
Response: title has been revised
Comment: Abstract is well written. However, results should be further specified specifically in quantitative values.
Response: The quantitative reporting of the results has been expanded.
Comment: Also add which mushroom was used for extraction of tyrosinase.
Response: The name of the mushroom has been updated.
Comment: Intro is very short; it needs modification for example the author should provide general background of the topic.
Response: The introduction has been expanded.
Comment: Intro should be start from the potential of medicinal plants in against various disease and specifically against skin diseases. The following studies could be helpful and cited.
Response: The potential of medicinal plants against various disease is highlighted in the first paragraph of the introduction.
Comment: Line 55 also add other references as the author used word researchers.
Response: The reference list has been updated to highlight other studies.
Comment: Line 66 also add other references as the author used word studies.
Response: The reference list has been updated to highlight other studies.
Comment: The author must discuss recent advances of the use of botanicals and plant extracts in enzyme inhibition.
Response: Comments on the use of computational studies in medicinal plant research have been added.
Comment: Why specific enzyme tyrosinase inhibition was used in this study provide the reason in introduction. What is its link with skin imperfection?
Response: Section on tyrosinase as a therapeutic target has been added and how it affects skin complexion.
Comment: Figure 1 is not very clear.
Response: The picture quality has been improved.
Comment: In conclusion present future recommendations.
Response: Future recommendations have been included.
