NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics

Lin, Penghui; Dai, Li; Crooks, Daniel R.; Neckers, Leonard M.; Higashi, Richard M.; Fan, Teresa W-M.; Lane, Andrew N.

doi:10.3390/metabo11040202

Open AccessArticle

NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics

by

Penghui Lin

¹

,

Li Dai

²

,

Daniel R. Crooks

²,

Leonard M. Neckers

²,

Richard M. Higashi

^1,3,

Teresa W-M. Fan

^1,3

and

Andrew N. Lane

^1,3,*

¹

Center for Environmental and Systems Biochemistry, University of Kentucky, 789 S. Limestone St, Lexington, KY 40536, USA

²

Urologic Oncology Branch, Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA

³

Department Toxicology & Cancer Biology, University of Kentucky, 789 S. Limestone St, Lexington, KY 40536, USA

^*

Author to whom correspondence should be addressed.

Metabolites 2021, 11(4), 202; https://doi.org/10.3390/metabo11040202

Submission received: 25 January 2021 / Revised: 23 March 2021 / Accepted: 26 March 2021 / Published: 29 March 2021

(This article belongs to the Special Issue Stable Isotope Guided Metabolomics)

Download

Browse Figures

Versions Notes

Abstract

Lipids comprise diverse classes of compounds that are important for the structure and properties of membranes, as high-energy fuel sources and as signaling molecules. Therefore, the turnover rates of these varied classes of lipids are fundamental to cellular function. However, their enormous chemical diversity and dynamic range in cells makes detailed analysis very complex. Furthermore, although stable isotope tracers enable the determination of synthesis and degradation of complex lipids, the numbers of distinguishable molecules increase enormously, which exacerbates the problem. Although LC-MS-MS (Liquid Chromatography-Tandem Mass Spectrometry) is the standard for lipidomics, NMR can add value in global lipid analysis and isotopomer distributions of intact lipids. Here, we describe new developments in NMR analysis for assessing global lipid content and isotopic enrichment of mixtures of complex lipids for two cell lines (PC3 and UMUC3) using both ¹³C₆ glucose and ¹³C₅ glutamine tracers.

Keywords: stable isotope tracers; Nuclear Magnetic Resonance; lipid ¹³C incorporation; isotopomer distributions

Share and Cite

MDPI and ACS Style

Lin, P.; Dai, L.; Crooks, D.R.; Neckers, L.M.; Higashi, R.M.; Fan, T.W.-M.; Lane, A.N. NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics. Metabolites 2021, 11, 202. https://doi.org/10.3390/metabo11040202

AMA Style

Lin P, Dai L, Crooks DR, Neckers LM, Higashi RM, Fan TW-M, Lane AN. NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics. Metabolites. 2021; 11(4):202. https://doi.org/10.3390/metabo11040202

Chicago/Turabian Style

Lin, Penghui, Li Dai, Daniel R. Crooks, Leonard M. Neckers, Richard M. Higashi, Teresa W-M. Fan, and Andrew N. Lane. 2021. "NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics" Metabolites 11, no. 4: 202. https://doi.org/10.3390/metabo11040202

APA Style

Lin, P., Dai, L., Crooks, D. R., Neckers, L. M., Higashi, R. M., Fan, T. W.-M., & Lane, A. N. (2021). NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics. Metabolites, 11(4), 202. https://doi.org/10.3390/metabo11040202

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Menu

NMR Methods for Determining Lipid Turnover via Stable Isotope Resolved Metabolomics

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Further Information

Guidelines

MDPI Initiatives

Follow MDPI