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Review

Isolation and Proteomics of the Insulin Secretory Granule

Charles Perkins Centre, School of Medical Sciences, University of Sydney, Camperdown, NSW 2006, Australia
*
Author to whom correspondence should be addressed.
Metabolites 2021, 11(5), 288; https://doi.org/10.3390/metabo11050288
Submission received: 12 April 2021 / Revised: 28 April 2021 / Accepted: 28 April 2021 / Published: 30 April 2021
(This article belongs to the Special Issue Islet Biology and Metabolism)

Abstract

Insulin, a vital hormone for glucose homeostasis is produced by pancreatic beta-cells and when secreted, stimulates the uptake and storage of glucose from the blood. In the pancreas, insulin is stored in vesicles termed insulin secretory granules (ISGs). In Type 2 diabetes (T2D), defects in insulin action results in peripheral insulin resistance and beta-cell compensation, ultimately leading to dysfunctional ISG production and secretion. ISGs are functionally dynamic and many proteins present either on the membrane or in the lumen of the ISG may modulate and affect different stages of ISG trafficking and secretion. Previously, studies have identified few ISG proteins and more recently, proteomics analyses of purified ISGs have uncovered potential novel ISG proteins. This review summarizes the proteins identified in the current ISG proteomes from rat insulinoma INS-1 and INS-1E cell lines. Here, we also discuss techniques of ISG isolation and purification, its challenges and potential future directions.
Keywords: insulin secretory granule; beta-cells; granule protein purification insulin secretory granule; beta-cells; granule protein purification

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MDPI and ACS Style

Norris, N.; Yau, B.; Kebede, M.A. Isolation and Proteomics of the Insulin Secretory Granule. Metabolites 2021, 11, 288. https://doi.org/10.3390/metabo11050288

AMA Style

Norris N, Yau B, Kebede MA. Isolation and Proteomics of the Insulin Secretory Granule. Metabolites. 2021; 11(5):288. https://doi.org/10.3390/metabo11050288

Chicago/Turabian Style

Norris, Nicholas, Belinda Yau, and Melkam Alamerew Kebede. 2021. "Isolation and Proteomics of the Insulin Secretory Granule" Metabolites 11, no. 5: 288. https://doi.org/10.3390/metabo11050288

APA Style

Norris, N., Yau, B., & Kebede, M. A. (2021). Isolation and Proteomics of the Insulin Secretory Granule. Metabolites, 11(5), 288. https://doi.org/10.3390/metabo11050288

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