Novel Chitin Deacetylase from Thalassiosira weissflogii Highlights the Potential for Chitin Derivative Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Culture
2.2. Substrate Preparation
2.3. Cloning and Sequence Analysis of TwCDA
2.4. Bioinformatic Analysis of TwCDA
2.5. Expression of Recombinant TwCDA Proteins
2.6. SDS-PAGE and Western Blotting
2.7. Detection of Chitin Deacetylase Activity
2.8. Detection of Chitinolytic Activity
2.9. Statistical Analysis
3. Results
3.1. Extraction and Analysis of Chitin from T. weissflogii
3.2. Sequence Analysis of TwCDA
3.3. Heterologous Expression and Deacetyl Activity of TwCDAs
3.4. Chitinolytic Activity of TwCDA
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Name | TwCDA | TwCDA-S |
---|---|---|
Molecular weight (Kda) | 71.15038 | 31.89025 |
Number of amino acids | 658 | 295 |
Length of CDS (bp) | 1974 | 885 |
Isoelectric point (pI) | 6.21 | 5.11 |
Instability index | 36.72 | 34.08 |
Signal peptide | 0 | 0 |
Number of predicted TMHs | 8 | 1 |
Conserved domain | Polysaccharide deacetylase | Polysaccharide deacetylase |
Subcellular location | ER | - |
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Cheng, M.; Shao, Z.; Wang, X.; Lu, C.; Li, S.; Duan, D. Novel Chitin Deacetylase from Thalassiosira weissflogii Highlights the Potential for Chitin Derivative Production. Metabolites 2023, 13, 429. https://doi.org/10.3390/metabo13030429
Cheng M, Shao Z, Wang X, Lu C, Li S, Duan D. Novel Chitin Deacetylase from Thalassiosira weissflogii Highlights the Potential for Chitin Derivative Production. Metabolites. 2023; 13(3):429. https://doi.org/10.3390/metabo13030429
Chicago/Turabian StyleCheng, Mengzhen, Zhanru Shao, Xin Wang, Chang Lu, Shuang Li, and Delin Duan. 2023. "Novel Chitin Deacetylase from Thalassiosira weissflogii Highlights the Potential for Chitin Derivative Production" Metabolites 13, no. 3: 429. https://doi.org/10.3390/metabo13030429