Next Article in Journal
Effects of Pera Orange Juice and Moro Orange Juice in Healthy Rats: A Metabolomic Approach
Next Article in Special Issue
Metabolic Alteration of MCF-7 Cells upon Indirect Exposure to E. coli Secretome: A Model of Studying the Microbiota Effect on Human Breast Tissue
Previous Article in Journal
Metabolomic Profiling of Aqueous Humor from Pathological Myopia Patients with Choroidal Neovascularization
Previous Article in Special Issue
Effects of Ecologically Relevant Concentrations of Cadmium on the Microbiota, Short-Chain Fatty Acids, and FFAR2 Expression in Zebrafish
 
 
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Communication

Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver

by
Amy M. Groswald
1,
Tyler C. Gripshover
2,
Walter H. Watson
1,3,4,5,
Banrida Wahlang
1,2,3,4,5,6,
Jianzhu Luo
1,3,
Loretta L. Jophlin
1,3,4,5 and
Matthew C. Cave
1,2,3,4,5,6,7,8,*
1
Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, School of Medicine, University of Louisville, Louisville, KY 40202, USA
2
Department of Pharmacology & Toxicology, School of Medicine, University of Louisville, Louisville, KY 40202, USA
3
The Hepatobiology and Toxicology Center, University of Louisville, Louisville, KY 40202, USA
4
The University of Louisville Alcohol Research Center, Louisville, KY 40202, USA
5
The Center for Integrative Environmental Health Sciences, Louisville, KY 40202, USA
6
The University of Louisville Superfund Research Center, Louisville, KY 40202, USA
7
Department of Biochemistry & Molecular Genetics, School of Medicine, University of Louisville, Louisville, KY 40202, USA
8
Robley Rex Veterans Affairs Medical Center, Louisville, KY 40206, USA
*
Author to whom correspondence should be addressed.
Metabolites 2023, 13(8), 901; https://doi.org/10.3390/metabo13080901
Submission received: 21 July 2023 / Revised: 27 July 2023 / Accepted: 28 July 2023 / Published: 1 August 2023
(This article belongs to the Special Issue Environmental Toxicology and Metabolism)

Abstract

Many pesticides have been identified as endocrine and metabolism-disrupting chemicals with hepatotoxic effects. However, data are limited for insecticides in the n-methyl carbamate class, including methomyl. Here, we investigate the liver and systemic metabolic effects of methomyl in a mouse model. We hypothesize that methomyl exposure will disrupt xenobiotic and intermediary metabolism and promote hepatic steatosis in mice. Male C57BL/6 mice were exposed daily to 0–5 mg/kg methomyl for 18 days. Mice were fed water and regular chow diet ad libitum. Metabolic phenotyping was performed, and tissue samples were collected. Effects were generally greatest at the highest methomyl dose, which induced Cyp1a2. Methomyl decreased whole body weight while the liver:body weight and testes:body weight ratios were increased. Hepatic steatosis increased while plasma LDL decreased. Fasting blood glucose and the glucose tolerance test area under the curve decreased along with hepatic glycogen stores. Methomyl, however, did not increase liver oxidative stress or injury. Collectively, these data demonstrate that methomyl disrupts hepatic xenobiotic and intermediary metabolism while increasing the testes:body weight ratio, suggesting that it may be an endocrine disrupting chemical. Besides methomyl’s known action in cholinesterase inhibition, it may be involved in aryl hydrocarbon receptor activation. The potential impact of n-methyl carbamate insecticides on metabolic health and diseases, including toxicant-associated steatotic liver disease (TASLD), warrants further investigation.
Keywords: toxicant-associated steatotic liver disease; toxicant-associated steatohepatitis; pesticide; environmental liver disease; endocrine disruptor; metabolic disrupting chemical toxicant-associated steatotic liver disease; toxicant-associated steatohepatitis; pesticide; environmental liver disease; endocrine disruptor; metabolic disrupting chemical

Share and Cite

MDPI and ACS Style

Groswald, A.M.; Gripshover, T.C.; Watson, W.H.; Wahlang, B.; Luo, J.; Jophlin, L.L.; Cave, M.C. Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver. Metabolites 2023, 13, 901. https://doi.org/10.3390/metabo13080901

AMA Style

Groswald AM, Gripshover TC, Watson WH, Wahlang B, Luo J, Jophlin LL, Cave MC. Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver. Metabolites. 2023; 13(8):901. https://doi.org/10.3390/metabo13080901

Chicago/Turabian Style

Groswald, Amy M., Tyler C. Gripshover, Walter H. Watson, Banrida Wahlang, Jianzhu Luo, Loretta L. Jophlin, and Matthew C. Cave. 2023. "Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver" Metabolites 13, no. 8: 901. https://doi.org/10.3390/metabo13080901

APA Style

Groswald, A. M., Gripshover, T. C., Watson, W. H., Wahlang, B., Luo, J., Jophlin, L. L., & Cave, M. C. (2023). Investigating the Acute Metabolic Effects of the N-Methyl Carbamate Insecticide, Methomyl, on Mouse Liver. Metabolites, 13(8), 901. https://doi.org/10.3390/metabo13080901

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop