Effects of Aging and Methionine Restriction on Rat Kidney Metabolome
Abstract
:1. Introduction
2. Results
2.1. Effects of Aging and MetR on Markers of Mitochondrial Stress and Tissue Protein Damage
2.2. Effect of Aging on the Renal Cortex Metabolome
2.3. Effect of MetR on the Renal Cortex Metabolome
3. Discussion
4. Materials and Methods
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Compound | Adult | Aged | MetR |
---|---|---|---|
2-SC | 69.55 ± 7.41 | 68.82 ± 7.80 | 72.58 ± 3.01 |
GSA | 5232.67 ± 458.74 | 5216.56 ± 178.61 | 5729.25 ± 376.60 |
AASA | 284.64 ± 37.76 | 276.84 ± 21.80 | 291.35 ± 21.36 |
CEL | 384.75 ± 42.28 | 322.70 ± 38.12 | 329.99 ± 22.78 |
MDAL | 228.53 ± 25.27 | 203.44 ± 25.44 | 204.02 ± 43.33 |
CMC | 103.78 ± 15.42 | 99.27 ± 9.04 | 103.43 ± 7.04 |
CML | 1595.83 ± 75.82 | 1410.95 ± 123.92 | 1494.69 ± 110.89 |
Compound | Adduct | m/z | RT | Log FC | p | Aged vs Adult |
---|---|---|---|---|---|---|
PC(P-34:2) a | M+H+ | 742.5863 | 7.64 | 7.83 | 0.0253 | up |
MAG(18:0) a* | M+Na+ | 381.2909 | 4.47 | 13.00 | 0.00159 | up |
DAG(32:2) a* | M+H+-H2O | 529.4520 | 8.41 | 7.02 | 0.0389 | up |
TAG(50:2) a | M+NH4+ | 848.7654 | 9.55 | 9.02 | 0.0134 | up |
TAG(56:8) a | M+H+ | 903.7554 | 9.78 | -8.74 | 0.0372 | down |
TAG(56:5) a | M+NH4+ | 926.8102 | 10.01 | 9.15 | 0.0242 | up |
Palmitaldehyde b | M+NH4+ | 258.2738 | 9.11 | 7.20 | 0.00955 | up |
EET methyl ester b | M+H+-H2O | 317.2477 | 11.83 | -6.73 | 0.0473 | down |
AA methyl ester b | M+NH4+ | 336.2908 | 11.80 | -6.85 | 0.0275 | down |
Dihomo-PGI2 b | M+H+-H2O | 363.2525 | 11.47 | -9.34 | 0.00401 | down |
ADP-ribose b | M+H+-H2O | 542.0597 | 0.58 | -9.47 | 0.0423 | down |
NADH b | M+Na+ | 688.1027 | 5.90 | -8.42 | 0.0418 | down |
Compound | m/z | RT | p value | LogFC MetR vs Aged | MetR vs Aged | LogFC MetR vs Adult | MetR vs Adult |
---|---|---|---|---|---|---|---|
LysoPE(18:3) * | 493.303 | 0.83 | 0.0197 | 10.67 | up | 5.68 | up |
LysoPI(15:0) | 559.2942 | 2.97 | 0.0234 | -9.06 | down | -0.03 | down |
PA(P-30:1) | 585.4237 | 6.41 | 0.00000662 | 14.26 | up | 12.35 | up |
PC(20:0) | 604.3236 | 2.13 | 0.0128 | 10.73 | up | 7.02 | up |
PC(38:6) * | 823.5946 | 5.58 | 0.00246 | 8.39 | up | 0.07 | up |
PC(42:2) | 870.688 | 7.40 | 0.0478 | -7.80 | down | -7.05 | down |
PC(P-38:2) | 820.6198 | 8.70 | 0.00248 | -7.57 | down | -0.07 | down |
PG(32:1) | 721.5002 | 7.72 | 0.0397 | 0.05 | up | -0.12 | down |
PS(P-29:0) | 642.453 | 5.67 | 0.03 | -5.80 | down | 1.62 | up |
GlcAβ-Cer(d36:1) | 742.5863 | 7.64 | 0.002 | -10.15 | down | -1.76 | down |
SM(d30:0) * | 666.5661 | 8.41 | 0.02 | 9.08 | up | 7.20 | up |
MAG(20:3) | 381.2906 | 4.48 | 0.00482 | -6.16 | down | 8.82 | up |
DAG(32:2) * | 529.4533 | 8.41 | 0.0173 | 0.61 | up | 7.37 | up |
TAG(40:2) | 848.7654 | 9.56 | 0.00188 | -10.11 | down | 1.85 | up |
TAG(61:6) | 941.8264 | 9.94 | 0.0235 | 11.11 | up | 1.74 | up |
Compound | m/z | RT | p value | LogFC MetR vs Aged | MetR vs Aged | LogFC MetR vs Adult | MetR vs Adult |
---|---|---|---|---|---|---|---|
Methylcytidine | 258.0986 | 0.48 | 0.0224 | -2.27 | down | 0.69 | up |
ADP-ribose | 542.0597 | 0.58 | 0.0036 | -0.02 | down | -2.36 | down |
Inosine | 269.0814 | 0.67 | 0.0291 | 0.01 | up | -0.12 | down |
Glutathione disulfide | 613.1499 | 0.46 | 0.0169 | -2.46 | down | -0.51 | down |
Angiotensin (1-7) | 937.4213 | 11.50 | 0.0375 | 2.29 | up | 2.58 | up |
epoxyHDHA | 399.2466 | 10.04 | 0.0183 | 2.62 | up | 0.48 | up |
10,11-DiHDPE | 363.2511 | 11.47 | 0.0236 | 2.35 | up | 0.05 | up |
20-carboxy-LTB4 | 367.2129 | 7.33 | 0.0143 | -0.11 | down | 0.00 | down |
6-keto PGE1 | 369.2334 | 6.89 | 0.0192 | -2.37 | down | -0.51 | down |
5β-Cholestane-3α,7α-diol | 427.3541 | 13.89 | 0.0242 | -0.07 | down | 1.98 | up |
Glycocholic Acid | 466.3105 | 8.93 | 0.000176 | 2.89 | up | 2.85 | up |
Dodecenoyl-CoA | 912.2679 | 13.69 | 0.0435 | -1.18 | down | 1.03 | up |
Formyl-CoA | 778.0916 | 10.56 | 0.0215 | 0.55 | up | -1.75 | down |
Palmitaldehyde | 258.2745 | 9.11 | 0.00461 | 0.01 | up | 2.69 | up |
Propionylcarnitine | 235.1639 | 9.19 | 0.0278 | 0.00 | up | 2.36 | up |
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Pradas, I.; Jové, M.; Cabré, R.; Ayala, V.; Mota-Martorell, N.; Pamplona, R. Effects of Aging and Methionine Restriction on Rat Kidney Metabolome. Metabolites 2019, 9, 280. https://doi.org/10.3390/metabo9110280
Pradas I, Jové M, Cabré R, Ayala V, Mota-Martorell N, Pamplona R. Effects of Aging and Methionine Restriction on Rat Kidney Metabolome. Metabolites. 2019; 9(11):280. https://doi.org/10.3390/metabo9110280
Chicago/Turabian StylePradas, Irene, Mariona Jové, Rosanna Cabré, Victoria Ayala, Natalia Mota-Martorell, and Reinald Pamplona. 2019. "Effects of Aging and Methionine Restriction on Rat Kidney Metabolome" Metabolites 9, no. 11: 280. https://doi.org/10.3390/metabo9110280