Targeted Isolation of Lignans from Trachelospermum asiaticum Using Molecular Networking and Hierarchical Clustering Analysis
Abstract
:1. Introduction
2. Results and Discussion
2.1. Molecular Networking and Hierarchical Clustering Analysis of Mass Spectral Data from T. asiaticum
2.2. Targeted Isolation of Compounds 1–5 Using Molecular Networking and HCA Results
2.3. Cytotoxic Activity of Isolated Compounds 1–5
3. Materials and Methods
3.1. Plant Material
3.2. Apparatus and Reagents
3.3. Extraction and Isolation
3.3.1. Trachelogenin (1)
3.3.2. Tracheloside (2)
3.3.3. Trachelogenin β-Gentionbioside (3)
3.3.4. Nortrachelogenin (4)
3.3.5. Nortracheloside (5)
3.4. GNPS Analysis
3.5. Dendrogram Analysis
3.6. Cytotoxicity Assay
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Compound | IC50 (μM) 1 | |||
---|---|---|---|---|
A549 | SKOV3 | PC3 | HEP2 | |
1 | 19.5 | 63.3 | 27.8 | 18.3 |
2 | 67.9 | 23.8 | 19.3 | >100 |
3 | 33.7 | 72.7 | 48.7 | 46.7 |
4 | 20.6 | 24.7 | 55.6 | 43.1 |
5 | 46.9 | 23.2 | 40.8 | 47.2 |
Etoposide 2 | 6.56 | 6.57 | 7.78 | 4.14 |
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Lee, J.; Yang, H.S.; Jeong, H.; Kim, J.-H.; Yang, H. Targeted Isolation of Lignans from Trachelospermum asiaticum Using Molecular Networking and Hierarchical Clustering Analysis. Biomolecules 2020, 10, 378. https://doi.org/10.3390/biom10030378
Lee J, Yang HS, Jeong H, Kim J-H, Yang H. Targeted Isolation of Lignans from Trachelospermum asiaticum Using Molecular Networking and Hierarchical Clustering Analysis. Biomolecules. 2020; 10(3):378. https://doi.org/10.3390/biom10030378
Chicago/Turabian StyleLee, Jiho, Hong Seok Yang, Hyogeun Jeong, Jung-Hwan Kim, and Heejung Yang. 2020. "Targeted Isolation of Lignans from Trachelospermum asiaticum Using Molecular Networking and Hierarchical Clustering Analysis" Biomolecules 10, no. 3: 378. https://doi.org/10.3390/biom10030378