Endogenous Collagenases Regulate Osteoclast Fusion
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Cells
2.2. Reagents and Antibodies
2.3. Osteoclast Differentiation and Measurement of Cell Size
2.4. Co-Culture of BMMs with Calvarial Cells
2.5. In Vitro Osteoclast Resorption Assay
2.6. The siRNA Knock-Down
2.7. Quantitative Real-Time PCR
2.8. Measurement of Osteoclasts in Calvarial Bones
2.9. Statistics
3. Results
3.1. The Role of Endogenous Collagenases in Osteoclastogenesis
3.2. The Effect of Collagenase Inhibition on Osteoclast Size
3.3. The Signaling Pathways by which the Inhibition of Collagenase Activities Regulate Osteoclast Size
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Kim, H.J.; Lee, Y. Endogenous Collagenases Regulate Osteoclast Fusion. Biomolecules 2020, 10, 705. https://doi.org/10.3390/biom10050705
Kim HJ, Lee Y. Endogenous Collagenases Regulate Osteoclast Fusion. Biomolecules. 2020; 10(5):705. https://doi.org/10.3390/biom10050705
Chicago/Turabian StyleKim, Hyo Jeong, and Youngkyun Lee. 2020. "Endogenous Collagenases Regulate Osteoclast Fusion" Biomolecules 10, no. 5: 705. https://doi.org/10.3390/biom10050705