BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals and Samples
2.2. Gene Expression Analysis
2.3. Immunohistochemical Determination of BAMBI and CHGA
2.4. Western Blot Analysis
2.5. Histopathological Study of Central Nervous System Tissues
2.6. Quantification of BAMBI and CHGA in Cerebrospinal Fluid
3. Results
3.1. Dysregulation of BAMBI and CHGA in the Central Nervous System of Scrapie Animals
3.2. Overexpression of BAMBI and CHGA Proteins in the Central Nervous System of Scrapie Animals
3.3. Western Blot Quantification of BAMBI and CHGA Proteins in the Central Nervous System of Scrapie-Affected Sheep
3.4. BAMBI and CHGA Expression Positively Correlates with Prion Neuropathology in Scrapie
3.5. BAMBI Is Upregulated in CSF of Scrapie-Affected Sheep at Clinical Stage and CJD Patients
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Sheep | Tg338 Mice | |||||||
---|---|---|---|---|---|---|---|---|
Preclinical Stage | Clinical Stage | Total Set | Scrapie Clinical + Preclinical | |||||
Control + Scrapie | Scrapie | Control + Scrapie | Scrapie | Control + Scrapie | Scrapie | |||
BAMBI | ||||||||
Spongiosis | 0.362 *** | 0.563 *** | 0.379 *** | 0.545 *** | 0.559 *** | 0.709 *** | 0.483 *** | 0.648 *** |
Intraneuronal vacuolation | 0.392 *** | 0.447 *** | ---- | ---- | ---- | ---- | ---- | ---- |
PrPSc deposition | 0.409 *** | 0.540 *** | ---- | N.S. | ---- | 0.454 ** | ---- | 0.327 * |
Microgliosis | 0.313 *** | 0.489 *** | 0.487 *** | 0.515 ** | 0.371 *** | 0.535 *** | 0.422 *** | 0.575 *** |
CHGA | ||||||||
Spongiosis | 0.431 *** | 0.564 *** | 0.518 *** | 0.625 *** | 0.668 *** | 0.694 *** | 0.594 *** | 0.694 *** |
Intraneuronal vacuolation | 0.345 *** | 0.476 *** | ---- | ---- | ---- | ---- | ---- | ---- |
PrPSc deposition | 0.507 *** | 0.530 *** | ---- | N.S. | ---- | 0.454 ** | ---- | 0.550 *** |
Microgliosis | 0.360 *** | 0.451 *** | 0.559 *** | 0.543 *** | 0.724 *** | 0.609 *** | 0.644 *** | 0.662 *** |
BAMBI/CHGA | 0.702 *** | 0.765 *** | 0.603 *** | 0.637 *** | 0.520 *** | 0.613 *** | 0.545 *** | 0.645 *** |
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López-Pérez, Ó.; Bernal-Martín, M.; Hernaiz, A.; Llorens, F.; Betancor, M.; Otero, A.; Toivonen, J.M.; Zaragoza, P.; Zerr, I.; Badiola, J.J.; et al. BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers. Biomolecules 2020, 10, 706. https://doi.org/10.3390/biom10050706
López-Pérez Ó, Bernal-Martín M, Hernaiz A, Llorens F, Betancor M, Otero A, Toivonen JM, Zaragoza P, Zerr I, Badiola JJ, et al. BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers. Biomolecules. 2020; 10(5):706. https://doi.org/10.3390/biom10050706
Chicago/Turabian StyleLópez-Pérez, Óscar, Marcos Bernal-Martín, Adelaida Hernaiz, Franc Llorens, Marina Betancor, Alicia Otero, Janne Markus Toivonen, Pilar Zaragoza, Inga Zerr, Juan José Badiola, and et al. 2020. "BAMBI and CHGA in Prion Diseases: Neuropathological Assessment and Potential Role as Disease Biomarkers" Biomolecules 10, no. 5: 706. https://doi.org/10.3390/biom10050706