Natural Product Isoliquiritigenin Activates GABAB Receptors to Decrease Voltage-Gate Ca2+ Channels and Glutamate Release in Rat Cerebrocortical Nerve Terminals
Abstract
:1. Introduction
2. Materials and Methods
2.1. Drugs
2.2. Animals
2.3. Preparation of Synaptosomes
2.4. Glutamate Release
2.5. Cytosolic Free Ca2+ Concentration ([Ca2+]C)
2.6. Immunohistochemistry
2.7. Molecular Docking Study
2.8. Western Blotting
2.9. Statistical Analysis
3. Results
3.1. ISL Inhibits 4-AP-evoked Glutamate Release from Nerve Terminals in the Cerebral Cortex through Ca2+ Influx Reduction
3.2. Specifics of VGCCs Involved in ISL Inhibition of Glutamate Release
3.3. Protein Kinase C Suppression Is Involved in the ISL-mediated Inhibition of Glutamate Release
3.4. Presynaptic GABAB Receptors Mediate ISL’s Effect on Glutamate Release
3.5. ISL Interacts with the GABAB Receptors
3.6. Gβγ-coupling Mechanism Is Involved in the ISL-mediated Inhibition of Glutamate Release
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Lin, T.-Y.; Lu, C.-W.; Hsieh, P.-W.; Chiu, K.-M.; Lee, M.-Y.; Wang, S.-J. Natural Product Isoliquiritigenin Activates GABAB Receptors to Decrease Voltage-Gate Ca2+ Channels and Glutamate Release in Rat Cerebrocortical Nerve Terminals. Biomolecules 2021, 11, 1537. https://doi.org/10.3390/biom11101537
Lin T-Y, Lu C-W, Hsieh P-W, Chiu K-M, Lee M-Y, Wang S-J. Natural Product Isoliquiritigenin Activates GABAB Receptors to Decrease Voltage-Gate Ca2+ Channels and Glutamate Release in Rat Cerebrocortical Nerve Terminals. Biomolecules. 2021; 11(10):1537. https://doi.org/10.3390/biom11101537
Chicago/Turabian StyleLin, Tzu-Yu, Cheng-Wei Lu, Pei-Wen Hsieh, Kuan-Ming Chiu, Ming-Yi Lee, and Su-Jane Wang. 2021. "Natural Product Isoliquiritigenin Activates GABAB Receptors to Decrease Voltage-Gate Ca2+ Channels and Glutamate Release in Rat Cerebrocortical Nerve Terminals" Biomolecules 11, no. 10: 1537. https://doi.org/10.3390/biom11101537
APA StyleLin, T. -Y., Lu, C. -W., Hsieh, P. -W., Chiu, K. -M., Lee, M. -Y., & Wang, S. -J. (2021). Natural Product Isoliquiritigenin Activates GABAB Receptors to Decrease Voltage-Gate Ca2+ Channels and Glutamate Release in Rat Cerebrocortical Nerve Terminals. Biomolecules, 11(10), 1537. https://doi.org/10.3390/biom11101537