A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Zein Protein Extraction
2.3. SDS-PAGE Analysis
2.4. In-Gel Digestion of Zein Proteins and Zip-Tip Procedure
2.5. Mass Spectrometric Analysis
2.6. Chemical Structure and Microtopography Analysis
3. Results
3.1. Proteomic Approach for Characterization of Zein Protein in Corn Flour Extraction
3.2. Chemical Structure and Microtopography Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Extraction Method | Maize Sample | Flour Granulation (µm) | Ethanol Concentration (%) | Protein Concentration (mg/mL) |
---|---|---|---|---|
Ultrasonic homogenizer | 65/250 | 250 | 65 | 1.58 |
65/710 | 710 | 65 | 2.09 | |
95/250 | 250 | 95 | 1.44 | |
95/710 | 710 | 95 | 1.90 | |
Ultrasonic bath | 65/250 | 250 | 65 | 0.79 |
65/710 | 710 | 65 | 0.63 | |
95/250 | 250 | 95 | 0.44 | |
95/710 | 710 | 95 | 0.28 |
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Darie-Ion, L.; Jayathirtha, M.; Hitruc, G.E.; Zaharia, M.-M.; Gradinaru, R.V.; Darie, C.C.; Pui, A.; Petre, B.A. A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction. Biomolecules 2021, 11, 1838. https://doi.org/10.3390/biom11121838
Darie-Ion L, Jayathirtha M, Hitruc GE, Zaharia M-M, Gradinaru RV, Darie CC, Pui A, Petre BA. A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction. Biomolecules. 2021; 11(12):1838. https://doi.org/10.3390/biom11121838
Chicago/Turabian StyleDarie-Ion, Laura, Madhuri Jayathirtha, Gabriela Elena Hitruc, Marius-Mihai Zaharia, Robert Vasile Gradinaru, Costel C. Darie, Aurel Pui, and Brindusa Alina Petre. 2021. "A Proteomic Approach to Identify Zein Proteins upon Eco-Friendly Ultrasound-Based Extraction" Biomolecules 11, no. 12: 1838. https://doi.org/10.3390/biom11121838