Diethylstilbestrol Modifies the Structure of Model Membranes and Is Localized Close to the First Carbons of the Fatty Acyl Chains
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Sample Preparation
2.3. Differential Scanning Calorimetry (DSC)
2.4. X-Ray Diffraction
2.5. 31P-NMR
2.6. 1H-NOESY-MAS-NMR
2.7. Molecular Dynamics Simulations
3. Results
3.1. DSC Study of the Interaction of DES with DMPC
3.2. DSC Study of the Interaction of DES with DEPE
3.3. X-Ray Diffraction
3.4. Changes in Phase of DEPE as Observed by SAXD and 31P-NMR
3.5. 1H-NOESY-MAS NMR
3.6. Molecular Dynamics Simulations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Molar Ratio | DMPC/DES |
---|---|
ΔH (kcal/mol) | |
1:0 | 6.5812 |
60:1 | 6.3835 |
30:1 | 6.3749 |
15:1 | 6.2677 |
7:1 | 6.198 |
3:1 | 5.4762 |
1:1 | 4.775 |
1:0 | 7.1094 |
80:1 | 6.8634 |
40:1 | 6.5521 |
20:1 | 4.5866 |
10:1 | 2.1381 |
d (Å) | zH (Å) | σC (Å) | dHH (Å) | dB (Å) | dw (Å) | |
---|---|---|---|---|---|---|
DMPC (8 °C) | 64.0 ± 0.2 | 19.5 ± 0.2 | 5.4 ± 0.2 | 39.0 ± 0.3 | 51.0 ± 1.2 | 13.0 ± 1.4 |
DMPC/DES (15:1) 8 °C | 70.9 ± 0.2 | 19.2 ± 0.1 | 5.9 ± 0.2 | 38.4 ± 0.2 | 50.4 ± 1.2 | 20.5 ± 1.3 |
DMPC/DES (7:1) 8 °C | 72.8 ± 0.2 | 19.1 ± 0.2 | 6.1 ± 0.3 | 38.2 ± 0.3 | 50.2 ± 1.3 | 22.6 ± 1.5 |
DMPC (30 °C) | 64.6 ± 0.2 | 18.0 ± 0.2 | 6.3 ± 0.2 | 36.0 ± 0.3 | 48.0 ± 1.2 | 16.6 ± 1.4 |
DMPC/DES (15:1) (30 °C) | 65.6 ± 0.1 | 17.5 ± 0.2 | 6.4 ± 0.2 | 35.0 ± 0.3 | 47.0 ± 1.2 | 18.6 ± 1.3 |
DMPC/DES (7:1) (30 °C) | 66.7 ± 0.1 | 17.5 ± 0.1 | 6.4 ± 0.1 | 35.0 ± 0.2 | 47.0 ± 1.1 | 19.7 ± 1.2 |
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Ausili, A.; Rodríguez-González, I.; Torrecillas, A.; Teruel, J.A.; Gómez-Fernández, J.C. Diethylstilbestrol Modifies the Structure of Model Membranes and Is Localized Close to the First Carbons of the Fatty Acyl Chains. Biomolecules 2021, 11, 220. https://doi.org/10.3390/biom11020220
Ausili A, Rodríguez-González I, Torrecillas A, Teruel JA, Gómez-Fernández JC. Diethylstilbestrol Modifies the Structure of Model Membranes and Is Localized Close to the First Carbons of the Fatty Acyl Chains. Biomolecules. 2021; 11(2):220. https://doi.org/10.3390/biom11020220
Chicago/Turabian StyleAusili, Alessio, Inés Rodríguez-González, Alejandro Torrecillas, José A. Teruel, and Juan C. Gómez-Fernández. 2021. "Diethylstilbestrol Modifies the Structure of Model Membranes and Is Localized Close to the First Carbons of the Fatty Acyl Chains" Biomolecules 11, no. 2: 220. https://doi.org/10.3390/biom11020220