Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. LEA Proteins
2.3. Sample Preparation
2.4. FTIR Spectroscopy
3. Results
3.1. FTIR Spectra of Dry Suc
3.2. The Presence of Buffer Components Influences the Properties of Dry Suc Glasses
3.3. Addition of Proteins Influences the Position of the νOH Peak of Suc in the Glassy State
3.4. Strength of H-Bonding Interactions in Dry Suc Glasses
3.5. Effect of Buffer Components and Proteins on Tg
3.6. Influence of Buffer Components and Proteins on the Fingerprint Region of FTIR Spectra from Dry Suc Glasses
3.7. The Presence of Both Buffer Components and Suc Influences the Extent of LEA Protein Aggregation in the Dry State
4. Discussion
4.1. Tris and Phosphate Buffer Components Increase Number and Strength of H-Bonding Interactions in Suc Glasses and Alter the Molecular Structure of Suc in the Dry State
4.2. LEA Proteins Stabilize Suc Glasses Dehydrated from Water or Tris Buffer and Influence the Molecular Structure of Dry Suc
4.3. The Desiccation-Induced Suc Glassy State Reduced Protein Aggregation of LEA Proteins
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
BSA | Bovine serum albumin |
FTIR | Fourier-transform infrared spectroscopy |
IDP | Intrinsically disordered protein |
LEA | Late embryogenesis abundant |
LG | β-lactoglobulin |
NaP | Sodium phosphate |
Suc | Sucrose |
TFE | 2,2,2-trifluoroethanol |
Tg | Glass transition temperature |
νOH | Vibration peak of OH groups |
WTCg | Wavenumber–temperature coefficient in the glassy state |
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Hincha, D.K.; Zuther, E.; Popova, A.V. Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana. Biomolecules 2021, 11, 615. https://doi.org/10.3390/biom11050615
Hincha DK, Zuther E, Popova AV. Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana. Biomolecules. 2021; 11(5):615. https://doi.org/10.3390/biom11050615
Chicago/Turabian StyleHincha, Dirk K., Ellen Zuther, and Antoaneta V. Popova. 2021. "Stabilization of Dry Sucrose Glasses by Four LEA_4 Proteins from Arabidopsis thaliana" Biomolecules 11, no. 5: 615. https://doi.org/10.3390/biom11050615