Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein Expression and Purification
2.2. Biomimetic Membranes
2.3. Sample Preparation
2.4. Polarized ATR-FTIR
3. Results & Discussion
3.1. Aggregation Behavior and Membrane Interaction Are Significantly Different for α- and β-Synuclein
3.2. Synuclein Interaction Prevents α-Synuclein Aggregation and Maintains Membrane Integrity
3.3. αS Δexon3 Shows Similar Effects to β-Synuclein but to a Lesser Extent
3.4. Orientation of Secondary Structure Elements upon Membrane Interaction Studied by Polarized Measurements
3.5. βS and Δ3 Prevent Membrane Damage by Counteracting αS Aggregation
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Scheibe, C.; Karreman, C.; Schildknecht, S.; Leist, M.; Hauser, K. Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation. Biomolecules 2021, 11, 1067. https://doi.org/10.3390/biom11081067
Scheibe C, Karreman C, Schildknecht S, Leist M, Hauser K. Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation. Biomolecules. 2021; 11(8):1067. https://doi.org/10.3390/biom11081067
Chicago/Turabian StyleScheibe, Christian, Christiaan Karreman, Stefan Schildknecht, Marcel Leist, and Karin Hauser. 2021. "Synuclein Family Members Prevent Membrane Damage by Counteracting α-Synuclein Aggregation" Biomolecules 11, no. 8: 1067. https://doi.org/10.3390/biom11081067