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Article

Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae

by
Randi Yeager
1,
G. Guy Bushkin
2,
Emily Singer
1,
Rui Fu
3,
Benjamin Cooperman
1 and
Michael McMurray
1,*
1
Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, Aurora, CO 80045, USA
2
Whitehead Institute for Biomedical Research, Cambridge, MA 02142, USA
3
RNA Biosciences Initiative, School of Medicine, University of Colorado, Aurora, CO 80045, USA
*
Author to whom correspondence should be addressed.
Biomolecules 2021, 11(8), 1223; https://doi.org/10.3390/biom11081223
Submission received: 16 July 2021 / Revised: 5 August 2021 / Accepted: 14 August 2021 / Published: 17 August 2021
(This article belongs to the Special Issue Transmembrane and Intracellular Signal Transduction Mechanisms: A Themed Issue in Honor of Professor Jeremy Thorner)
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Abstract

Gametogenesis in diploid cells of the budding yeast Saccharomyces cerevisiae produces four haploid meiotic products called spores. Spores are dormant until nutrients trigger germination, when they bud asexually or mate to return to the diploid state. Each sporulating diploid produces a mix of spores of two haploid mating types, a and α. In asexually dividing haploids, the mating types result from distinct, mutually exclusive gene expression programs responsible for production of mating pheromones and the receptors to sense them, all of which are silent in diploids. It was assumed that spores only transcribe haploid- and mating-type-specific genes upon germination. We find that dormant spores of each mating type harbor transcripts representing all these genes, with the exception of Mata1, which we found to be enriched in a spores. Mata1 transcripts, from a rare yeast gene with two introns, were mostly unspliced. If the retained introns reflect tethering to the MATa locus, this could provide a mechanism for biased inheritance. Translation of pheromones and receptors were repressed at least until germination. We find antisense transcripts to many mating genes that may be responsible. These findings add to the growing number of examples of post-transcriptional regulation of gene expression during gametogenesis.
Keywords: budding yeast; sporulation; gametogenesis; gene expression; splicing; antisense budding yeast; sporulation; gametogenesis; gene expression; splicing; antisense

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MDPI and ACS Style

Yeager, R.; Bushkin, G.G.; Singer, E.; Fu, R.; Cooperman, B.; McMurray, M. Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae. Biomolecules 2021, 11, 1223. https://doi.org/10.3390/biom11081223

AMA Style

Yeager R, Bushkin GG, Singer E, Fu R, Cooperman B, McMurray M. Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae. Biomolecules. 2021; 11(8):1223. https://doi.org/10.3390/biom11081223

Chicago/Turabian Style

Yeager, Randi, G. Guy Bushkin, Emily Singer, Rui Fu, Benjamin Cooperman, and Michael McMurray. 2021. "Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae" Biomolecules 11, no. 8: 1223. https://doi.org/10.3390/biom11081223

APA Style

Yeager, R., Bushkin, G. G., Singer, E., Fu, R., Cooperman, B., & McMurray, M. (2021). Post-Transcriptional Control of Mating-Type Gene Expression during Gametogenesis in Saccharomyces cerevisiae. Biomolecules, 11(8), 1223. https://doi.org/10.3390/biom11081223

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