Attenuation of PITPNM1 Signaling Cascade Can Inhibit Breast Cancer Progression
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. SiRNA Transfection
2.3. CCK8 Cell Counting Assay
2.4. Colony Formation Assay
2.5. RNA Isolation and Real-Time Quantitative PCR
2.6. Data Extraction and Bioinformatics Analysis
2.7. Statistical Analysis
3. Results
3.1. PITPNM1 Is Significantly Higher in Breast Cancer and Correlates with Poor Prognosis in Patients with Breast Cancer
3.2. PITPNM1 Over-Expression Is Associated with Carcinogenesis Gene Ontology and Pathway
3.3. Silencing of PITPNM1 Inhibits Proliferation of Breast Cancer Cells
3.4. The Relationship between PITPNM1 Expression and T Cell Populations
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Liu, Z.; Shi, Y.; Lin, Q.; Yang, W.; Luo, Q.; Cen, Y.; Li, J.; Fang, X.; Jiang, W.G.; Gong, C. Attenuation of PITPNM1 Signaling Cascade Can Inhibit Breast Cancer Progression. Biomolecules 2021, 11, 1265. https://doi.org/10.3390/biom11091265
Liu Z, Shi Y, Lin Q, Yang W, Luo Q, Cen Y, Li J, Fang X, Jiang WG, Gong C. Attenuation of PITPNM1 Signaling Cascade Can Inhibit Breast Cancer Progression. Biomolecules. 2021; 11(9):1265. https://doi.org/10.3390/biom11091265
Chicago/Turabian StyleLiu, Zihao, Yu Shi, Qun Lin, Wenqian Yang, Qing Luo, Yinghuan Cen, Juanmei Li, Xiaolin Fang, Wen G. Jiang, and Chang Gong. 2021. "Attenuation of PITPNM1 Signaling Cascade Can Inhibit Breast Cancer Progression" Biomolecules 11, no. 9: 1265. https://doi.org/10.3390/biom11091265