Anti-Inflammatory Effects of Encapsulated Human Mesenchymal Stromal/Stem Cells and a Method to Scale-Up Cell Encapsulation
Abstract
:1. Introduction
2. Materials and Methods
2.1. Encapsulated Human MSC (eMSC)
2.2. Spinal Cord Injury (SCI) and Capsule Injection
2.3. ELISA and Multiplex Assays
2.4. qRT-PCR
2.5. Rat Endotoxemia Model
2.6. Rapid Capsule Collection System (RaCCS)
2.7. Statistical Analysis
3. Results
3.1. Effects of Encapsulated MSC In Vivo
3.1.1. Encapsulated MSC in SCI
3.1.2. eMSC in Rat Endotoxemia
3.1.3. Viability of MSC in Unpolymerized Alginate
3.2. A Rapid Capsule Collection System (RaCCS) for Scaling-Up Cell Encapsulation
3.2.1. A Rapid Capsules Collection System (RaCCS)
3.2.2. Properties of Encapsulation
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Appendix A
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Kumar, S.; Kabat, M.; Basak, S.; Babiarz, J.; Berthiaume, F.; Grumet, M. Anti-Inflammatory Effects of Encapsulated Human Mesenchymal Stromal/Stem Cells and a Method to Scale-Up Cell Encapsulation. Biomolecules 2022, 12, 1803. https://doi.org/10.3390/biom12121803
Kumar S, Kabat M, Basak S, Babiarz J, Berthiaume F, Grumet M. Anti-Inflammatory Effects of Encapsulated Human Mesenchymal Stromal/Stem Cells and a Method to Scale-Up Cell Encapsulation. Biomolecules. 2022; 12(12):1803. https://doi.org/10.3390/biom12121803
Chicago/Turabian StyleKumar, Suneel, Maciej Kabat, Sayantani Basak, Joanne Babiarz, Francois Berthiaume, and Martin Grumet. 2022. "Anti-Inflammatory Effects of Encapsulated Human Mesenchymal Stromal/Stem Cells and a Method to Scale-Up Cell Encapsulation" Biomolecules 12, no. 12: 1803. https://doi.org/10.3390/biom12121803