Joint Action Toxicity of Arsenic (As) and Lead (Pb) Mixtures in Developing Zebrafish
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal Husbandry
2.2. Arsenic and Lead Single Chemical Exposure
2.3. Single Arsenic and Lead LC Calculations
2.4. Binary Arsenic and Lead Mixture Exposure
2.5. Independent Action (IA) and Concentration Addition (CA) Model Calculations
2.6. Mixture Toxicity Analysis and Comparison of Measured with Predicted Toxicity
2.7. Type of Mixture Interaction Analysis
3. Results
3.1. Effects of Single Chemical Exposure on Mortality
Chemical | Percent (%) Mortality | ||||
---|---|---|---|---|---|
0% | 25% | 50% | 75% | 100% | |
Lead (Pb) | 0 ppm | 39.0 ppm | 73.76 ppm | 99.94 ppm | 121.74 ppm |
Arsenic (As) | 0 ppm | 40.18 ppm | 55.42 ppm | 66.64 ppm | 77.32 pm |
3.2. Effects of Metal Mixtures on Mortality
3.3. Mixture Toxicity Comparison of Predicted to Observed Toxicity
3.4. Type of Mixture Interaction Analysis
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Experiment | Mixture 1 | Mixture 2 | Mixture 3 |
---|---|---|---|
Experiment 1 | Pb LC25 + As LC25 | Pb LC25 + As LC50 | Pb LC25 + As LC75 |
Experiment 2 | Pb LC50 + As LC25 | Pb LC50 + As LC50 | Pb LC50 + As LC75 |
Experiment 3 | Pb LC75 + As LC25 | Pb LC75 + As LC50 | Pb LC75 + As LC75 |
Mixture Treatments | Observed vs. CA | Observed vs. IA | ||
---|---|---|---|---|
x2 | p | x2 | p | |
Pb LC25 + As LC25 | 22.427 | 0.0001 | 20.74 | 0.0001 |
Pb LC25 + As LC50 | 3.209 | 0.0733 | - | - |
Pb LC25 + As LC75 | 0.328 | 0.5666 | - | - |
Pb LC50 + As LC25 | 48 | <0.0001 | - | - |
Pb LC50 + As LC50 | 50 | <0.0001 | 50 | 0.0001 |
Pb LC50 + As LC75 | 33.333 | <0.0001 | - | - |
Pb LC75 + As LC25 | 50 | <0.0001 | - | - |
Pb LC75+ As LC50 | 25 | <0.0001 | - | - |
Pb LC75 + As LC75 | 15.97 | <0.0001 | 15.789 | 0.0001 |
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Kiper, K.; Freeman, J.L. Joint Action Toxicity of Arsenic (As) and Lead (Pb) Mixtures in Developing Zebrafish. Biomolecules 2022, 12, 1833. https://doi.org/10.3390/biom12121833
Kiper K, Freeman JL. Joint Action Toxicity of Arsenic (As) and Lead (Pb) Mixtures in Developing Zebrafish. Biomolecules. 2022; 12(12):1833. https://doi.org/10.3390/biom12121833
Chicago/Turabian StyleKiper, Keturah, and Jennifer L. Freeman. 2022. "Joint Action Toxicity of Arsenic (As) and Lead (Pb) Mixtures in Developing Zebrafish" Biomolecules 12, no. 12: 1833. https://doi.org/10.3390/biom12121833