Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling
Abstract
:1. Introduction
2. Material and Methods
2.1. Isolation and Cultivation of BM-MSCs
2.2. Characterization of BM-MSCs
2.2.1. Immunophenotype Analysis
2.2.2. In Vitro Multilineage Differentiation
2.3. CFU–F (Colony Forming Unit–Fibroblast) Assay
2.4. Cellular Viability and Cell Cycle Progression
2.5. Osteogenic and Adipogenic Differentiation of BM-MSCs
2.6. Immunofluorescence Assay
2.7. β-Galactosidase Staining
2.8. Quantitative Real-Time PCR (qRT-PCR)
2.9. Statistical Analysis
3. Results
3.1. Bone Marrow Mesenchymal Stromal/Stem Cells’ Characterization
3.2. Influence of VD3 on BM-MSCs’ Proliferation and Differentiation
3.3. VD3 Activates SIRT1-FoxO3 Signaling Pathways in BM-MSCs
3.4. VD3 Treatment Modulates Proliferation and Cell Cycle Progression of BM-MSCs Independently of SIRT1 Signaling
3.5. VD3 Treatment Modulates the Expression of Pluripotency-Associated Markers in BM-MSCs: Involvement of SIRT1 Signaling
3.6. Modulated Differentiation Potential of VD3-Pretreated BM-MSCs: Involvement of SIRT1 Signaling
3.7. VD3 Treatment Reduces β-Galactosidase Expression in BM-MSCs: The Role of SIRT1 Signaling
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Gene | Sequence (5′-3′) |
---|---|
GAPDH | F: GAAGGTGAAGGTCGGAGT |
R: GAAGATGGTGATGGGATTTC | |
ALP | F: CACCCACGTCGATTGCATCT |
R: TAGCCACGTTGGTGTTGAGC | |
OCN | F: GGCGCTACCTGTATCAATGG |
R: TCAGCCAACTCGTCACAGTC | |
RUNX2 | F: GCCTAGGCGCATTTCAGA |
R: CTGAGAGTGGAAGGCCAGAG | |
NANOG | F: GATGCCTCACACGGAGACTG |
R: GCAGAAGTGGGTTGTTTGCC | |
Oct4 | F: TGAGTAGTCCCTTCGCAAGC |
R. GCTTCGGATTTCGCCTTCTC | |
SOX2 | F: GACAGTTACGCGCACATGAA |
R: TAGGTCTGCGAGCTGGTCAT | |
SIRT1 | F: TGCTGGCCTAATAGAGTGGCA |
R: CTCAGCGCCATGGAAAATGT |
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Borojević, A.; Jauković, A.; Kukolj, T.; Mojsilović, S.; Obradović, H.; Trivanović, D.; Živanović, M.; Zečević, Ž.; Simić, M.; Gobeljić, B.; et al. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. Biomolecules 2022, 12, 323. https://doi.org/10.3390/biom12020323
Borojević A, Jauković A, Kukolj T, Mojsilović S, Obradović H, Trivanović D, Živanović M, Zečević Ž, Simić M, Gobeljić B, et al. Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. Biomolecules. 2022; 12(2):323. https://doi.org/10.3390/biom12020323
Chicago/Turabian StyleBorojević, Ana, Aleksandra Jauković, Tamara Kukolj, Slavko Mojsilović, Hristina Obradović, Drenka Trivanović, Milena Živanović, Željko Zečević, Marija Simić, Borko Gobeljić, and et al. 2022. "Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling" Biomolecules 12, no. 2: 323. https://doi.org/10.3390/biom12020323
APA StyleBorojević, A., Jauković, A., Kukolj, T., Mojsilović, S., Obradović, H., Trivanović, D., Živanović, M., Zečević, Ž., Simić, M., Gobeljić, B., Vujić, D., & Bugarski, D. (2022). Vitamin D3 Stimulates Proliferation Capacity, Expression of Pluripotency Markers, and Osteogenesis of Human Bone Marrow Mesenchymal Stromal/Stem Cells, Partly through SIRT1 Signaling. Biomolecules, 12(2), 323. https://doi.org/10.3390/biom12020323