Thiophosphate Analogs of Coenzyme A and Its Precursors—Synthesis, Stability, and Biomimetic Potential
Abstract
:1. Introduction
2. Materials and Methods
2.1. Protein Expression and Purification
2.2. Enzymatic Synthesis of Thiophosphate Analogs of PPanSH, dpCoA, and CoA
2.3. Synthesis of Calcium Hopantenate (HoPan)
2.4. Serum Stability of C1
2.5. Pyrophosphate Hydrolysis in Fetal Bovine Serum (FBS)
2.6. Pyrophosphate Hydrolysis by Human Nudt7
2.7. HEK 293T Cell Experiments
3. Results
3.1. Enzymatic Synthesis of Thiophosphate Analogs
3.2. Stability of Thiophosphate Analogs
3.3. C1 Treatment Rescues CoA-Deficient Cells
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Löcherer, C.; Tosun, E.; Backes, H.; Jäschke, A. Thiophosphate Analogs of Coenzyme A and Its Precursors—Synthesis, Stability, and Biomimetic Potential. Biomolecules 2022, 12, 1065. https://doi.org/10.3390/biom12081065
Löcherer C, Tosun E, Backes H, Jäschke A. Thiophosphate Analogs of Coenzyme A and Its Precursors—Synthesis, Stability, and Biomimetic Potential. Biomolecules. 2022; 12(8):1065. https://doi.org/10.3390/biom12081065
Chicago/Turabian StyleLöcherer, Christian, Elif Tosun, Hannah Backes, and Andres Jäschke. 2022. "Thiophosphate Analogs of Coenzyme A and Its Precursors—Synthesis, Stability, and Biomimetic Potential" Biomolecules 12, no. 8: 1065. https://doi.org/10.3390/biom12081065