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Open AccessArticle
Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes
by
Richard F. Kraus
Richard F. Kraus 1,*,
Daniel Panter
Daniel Panter 1,
Michael A. Gruber
Michael A. Gruber 1,
Stephanie Arndt
Stephanie Arndt 2,
Petra Unger
Petra Unger 2,
Michael T. Pawlik
Michael T. Pawlik 3 and
Diane Bitzinger
Diane Bitzinger 1
1
Department of Anaesthesiology, University Medical Centre Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany
2
Department of Dermatology, University Medical Centre Regensburg, Franz-Josef-Strauss-Allee 11, 93053 Regensburg, Germany
3
Center of Dive and Hyperbaric Medicine, Department of Anaesthesiology, Caritas Hospital St. Josef, Landshuter Str. 65, 93053 Regensburg, Germany
*
Author to whom correspondence should be addressed.
Biomolecules 2024, 14(10), 1242; https://doi.org/10.3390/biom14101242 (registering DOI)
Submission received: 24 July 2024
/
Revised: 14 September 2024
/
Accepted: 16 September 2024
/
Published: 30 September 2024
Abstract
Background: The application of normo- and hyperbaric O2 is a common therapy option in various disease patterns. Thereby, the applied O2 affects the whole body, including the blood and its components. This study investigates influences of pressure and oxygen fraction on human blood plasma, nutrient media, and the functions of neutrophil granulocytes (PMNs). Methods: Neutrophil migration, reactive oxygen species (ROS) production, and NETosis were examined by live cell imaging. The treatment of various matrices (Roswell Park Memorial Institute 1640 medium, Dulbecco’s Modified Eagle’s Medium, H2O, human plasma, and isolated PMNs) with hyperbaric oxygen (HBO) was performed. In addition, the expression of different neutrophil surface epitopes (CD11b, CD62L, CD66b) and the oxidative burst were investigated by flow cytometry (FACS). The application of cold atmospheric plasma (CAP) to normoxic and normobaric culture media served as a positive control. Soluble reaction products such as H2O2, reactive nitrogen species (RNS: NO2− and NO3−), and ROS-dependent dihydrorhodamine oxidation were quantified by fluoro- and colorimetric assay kits. Results: Under normobaric normoxia, PMNs migrate slower and shorter in comparison with normobaric hyper- or hypoxic conditions and hyperbaric hyperoxia. The pressure component has less effect on the migration behavior of PMNs than the O2 concentration. Individual PMN cells produce prolonged ROS at normoxic conditions. PMNs showed increased expression of CD11b in normobaric normoxia, lower expression of CD62L in normobaric normoxia, and lower expression of CD66b after HBO and CAP treatment. Treatment with CAP increased the amount of ROS and RNS in common culture media. Conclusions: Hyperbaric and normobaric O2 influences neutrophil functionality and surface epitopes in a measurable way, which may have an impact on disorders with neutrophil involvement. In the context of hyperbaric experiments, especially high amounts of H2O2 in RPMI after hyperbaric oxygen should be taken into account. Therefore, our data support a critical indication for the use of normobaric and hyperbaric oxygen and conscientious and careful handling of oxygen in everyday clinical practice.
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MDPI and ACS Style
Kraus, R.F.; Panter, D.; Gruber, M.A.; Arndt, S.; Unger, P.; Pawlik, M.T.; Bitzinger, D.
Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes. Biomolecules 2024, 14, 1242.
https://doi.org/10.3390/biom14101242
AMA Style
Kraus RF, Panter D, Gruber MA, Arndt S, Unger P, Pawlik MT, Bitzinger D.
Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes. Biomolecules. 2024; 14(10):1242.
https://doi.org/10.3390/biom14101242
Chicago/Turabian Style
Kraus, Richard F., Daniel Panter, Michael A. Gruber, Stephanie Arndt, Petra Unger, Michael T. Pawlik, and Diane Bitzinger.
2024. "Effects of Pressure, Hypoxia, and Hyperoxia on Neutrophil Granulocytes" Biomolecules 14, no. 10: 1242.
https://doi.org/10.3390/biom14101242
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