Next Article in Journal
From Structure to Function: Analysis of the First Monomeric Pyridoxal-5′-Phosphate-Dependent Transaminase from the Bacterium Desulfobacula toluolica
Previous Article in Journal
Assessment of Agrimonia eupatoria L. and Lipophosphonoxin (DR-6180) Combination for Wound Repair: Bridging the Gap Between Phytomedicine and Organic Chemistry
 
 
Search for Articles:
Title / Keyword
Author / Affiliation / Email
Journal
Article Type
 
 
Advanced Search
 
Section
Special Issue
Volume
Issue
Number
Page
 
Journals
Biomolecules
Volume 14
Issue 12
10.3390/biom14121589
biomolecules-logo
Submit to this Journal Review for this Journal Propose a Special Issue
► Article Menu

Article Menu

share Share announcement Help format_quote Cite question_answer Discuss in SciProfiles
first_page
settings
Order Article Reprints
Font Type:
Arial Georgia Verdana
Font Size:
Aa Aa Aa
Line Spacing:
Column Width:
Background:
Article

Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White

by
Liliya I. Mukhametova
1,
Dmitry O. Zherdev
1,
Sergei A. Eremin
1,
Pavel A. Levashov
1,
Hans-Christian Siebert
2,
Yury E. Tsvetkov
3,
Olga N. Yudina
3,
Vadim B. Krylov
4,* and
Nikolay E. Nifantiev
3,*
1
Faculty of Chemistry, M.V. Lomonosov Moscow State University, Leninsky Gory 1/3, 119991 Moscow, Russia
2
RI-B-NT—Research Institute of Bioinformatics and Nanotechnology, Schauenburger Str. 116, 24118 Kiel, Germany
3
Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
4
Laboratory of Synthetic Glycovaccines, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospect 47, 119991 Moscow, Russia
*
Authors to whom correspondence should be addressed.
Biomolecules 2024, 14(12), 1589; https://doi.org/10.3390/biom14121589
Submission received: 18 October 2024 / Revised: 2 December 2024 / Accepted: 9 December 2024 / Published: 12 December 2024
(This article belongs to the Special Issue Recent Advances in Fluorescent Probes: From Structure Design to Biological Applications)
Browse Figures
Versions Notes

Abstract

This study describes the applicability of the fluorescence polarization assay (FPA) based on the use of FITC-labeled oligosaccharide tracers of defined structure for the measurement of active lysozyme in hen egg white. Depending on the oligosaccharide chain length of the tracer, this method detects both the formation of the enzyme-to-tracer complex (because of lectin-like, i.e., carbohydrate-binding action of lysozyme) and tracer splitting (because of chitinase activity of lysozyme). Evaluation of the fluorescence polarization dynamics enables simultaneous measurement of the chitinase and lectin activities of lysozyme, which is crucial for its detection in complex biological systems. Hen egg white lysozyme (HEWL), unlike human lysozyme (HL), formed a stable complex with the chitotriose tracer that underwent no further transformations. This fact allows for easy measurement of the carbohydrate-binding activity of the HEWL. The results of the lysozyme activity measurement for hen egg samples obtained through the FPA correlated with the results obtained using the traditional turbidimetry method. The FPA does not have the drawbacks of turbidimetry, which are associated with the need to use bacterial cells that cannot be precisely standardized. Additionally, FPA offers advantages such as rapid analysis, the use of compact equipment, and standardized reagents. Therefore, the new express technique for measuring the lysozyme activity is applicable for evaluating the complex biomaterial, including for the purposes of food product quality control.
Keywords: lysozyme; activity; fluorescence polarization; fluorescence polarization assay lysozyme; activity; fluorescence polarization; fluorescence polarization assay
Graphical Abstract

Share and Cite

MDPI and ACS Style

Mukhametova, L.I.; Zherdev, D.O.; Eremin, S.A.; Levashov, P.A.; Siebert, H.-C.; Tsvetkov, Y.E.; Yudina, O.N.; Krylov, V.B.; Nifantiev, N.E. Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White. Biomolecules 2024, 14, 1589. https://doi.org/10.3390/biom14121589

AMA Style

Mukhametova LI, Zherdev DO, Eremin SA, Levashov PA, Siebert H-C, Tsvetkov YE, Yudina ON, Krylov VB, Nifantiev NE. Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White. Biomolecules. 2024; 14(12):1589. https://doi.org/10.3390/biom14121589

Chicago/Turabian Style

Mukhametova, Liliya I., Dmitry O. Zherdev, Sergei A. Eremin, Pavel A. Levashov, Hans-Christian Siebert, Yury E. Tsvetkov, Olga N. Yudina, Vadim B. Krylov, and Nikolay E. Nifantiev. 2024. "Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White" Biomolecules 14, no. 12: 1589. https://doi.org/10.3390/biom14121589

APA Style

Mukhametova, L. I., Zherdev, D. O., Eremin, S. A., Levashov, P. A., Siebert, H.-C., Tsvetkov, Y. E., Yudina, O. N., Krylov, V. B., & Nifantiev, N. E. (2024). Application of the Chitooligosaccharides and Fluorescence Polarization Technique for the Assay of Active Lysozyme in Hen Egg White. Biomolecules, 14(12), 1589. https://doi.org/10.3390/biom14121589

Note that from the first issue of 2016, this journal uses article numbers instead of page numbers. See further details here.

Article Metrics

Back to TopTop