Unraveling the Etiology of Dilated Cardiomyopathy through Differential miRNA–mRNA Interactome
Abstract
:1. Introduction
2. Materials and Methods
2.1. Study Population
2.2. Myocardial Tissue Collection
2.3. RNA Isolation and qRT-PCR
2.4. RNA-Seq Analysis and Bioinformatics
2.5. Luciferase Reporter Assay
2.6. Statistical Analysis
3. Results
3.1. Baseline Characteristics
3.2. VCM and ICM Have Distinct mRNA and miRNA Expression Profiles
3.3. miRNA–Target Transcript Interaction Network
3.4. Validation of Differentially Expressed Genes and miRNAs by qRT-PCR
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene | Forward | Reverse |
---|---|---|
DDX6 | 5′-AATACTGAACTATGGACCTATGAGCA-3′ | 5′-TTGCAGGGCTCACACTAGG-3′ |
SEMA4A | 5′-TGGGGACTACTCTGCCTACTACA-3′ | 5′-GGGTTACTCTGCTCCATGTCA-3′ |
SGMS2 | 5′-AGCACGTGCACAGCTTCA-3′ | 5′-GTCCACGGGTGAAACAGC-3′ |
TTC39C | 5′-TCTGGACAAGTACAATGCTGAGA-3′ | 5′-TAAGCTTCGCTGCACAGGT-3′ |
GAPDH | 5′-AGCCACATCGCTCAGACAC-3′ | 5′-AATACGACCAAATCCGTTGACT-3′ |
ß-ACTIN | 5′-TGTGGCATCCACGAAACTACC-3′ | 5′-CTCAGGAGGAGCAATGATCTTGAT-3′ |
Variable | ICM (N = 6) | VCM (N = 9) | p-Value |
---|---|---|---|
Age (years), means ± SD | 66.33 ± 7.84 | 63.67 ± 11.78 | 0.637 |
Sex (male, %) | 100 | 88.89 | 0.699 |
LVEF (%) | 38 ± 15.87 | 48.13 ± 10.88 | 0.164 |
LVEDD (mm) | 60.33 ± 2.16 | 60 ± 5.15 | 0.885 |
LVESD (mm) | 44 ± 2.16 | 44.13± 7.62 | 0.969 |
LA (mm) | 47.50 ± 2.65 | 52 ± 5.61 | 0.092 |
High blood pressure (%) | 66.67 | 88.89 | 0.574 |
Dyslipidemia (%) | 66.67 | 55.56 | 0.975 |
Diabetes Mellitus (%) | 50 | 66.67 | 0.519 |
ACEI/ARAII (y/n, %) | 83.33 | 77.77 | 0.792 |
Diuretics (y/n, %) | 83.33 | 88.89 | 0.757 |
Calcium antagonist (y/n, %) | 16.67 | 22.22 | 0.792 |
Statins (y/n, %) | 83.33 | 77.78 | 0.792 |
Ezetrol (%) | 16.67 | 22.22 | 0.792 |
Metformin (%) | 33.33 | 33.33 | >0.999 |
Metformin/IGP4 (%) | 16.67 | 22.22 | 0.792 |
Insulin (%) | 16.67 | 22.22 | 0.792 |
Aspirin (y/n, %) | 100 | 22.22 | 0.003 |
Beta-blocker (%) | 83.33 | 77.78 | 0.792 |
Database | Mature Mirna acc | Mature Mirna ID | Target Symbol | Target Entrez | Target Ensembl | Type | Pubmed ID | Score |
---|---|---|---|---|---|---|---|---|
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | ATM | 472 | ENSG00000149311 | validated | 23212916 | |
mirtarbase | MIMAT0002816 | hsa-miR-494-3p | BCL2 | 596 | ENSG00000171791 | validated | 24960059 | |
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | DDX6 | 1656 | ENSG00000110367 | validated | 23212916 | |
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | GOLGA3 | 2802 | ENSG00000090615 | validated | 23212916 | |
mirtarbase | MIMAT0002816 | hsa-miR-494-3p | REST | 5978 | ENSG00000084093 | validated | 23446348 | |
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | KCNK6 | 9424 | ENSG00000099337 | validated | 23313552 | |
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | ARSG | 22901 | ENSG00000141337 | validated | 23622248 | |
mirtarbase | MIMAT0000275 | hsa-miR-218-5p | MB21D2 | 151963 | ENSG00000180611 | validated | 23212916 | |
tarbase | MIMAT0000275 | hsa-miR-218-5p | REST | 5978 | ENSG00000084093 | validated | 20371350 | |
tarbase | MIMAT0003180 | hsa-miR-487b-3p | SGMS2 | 166929 | ENSG00000164023 | validated | 24038734 | |
tarbase | MIMAT0002816 | hsa-miR-494-3p | CPNE1 | 8904 | ENSG00000214078 | validated | 25653011 | |
tarbase | MIMAT0004672 | hsa-miR-106b-3p | SCPEP1 | 59342 | ENSG00000121064 | validated | 22291592 | |
diana_microt | MIMAT0000275 | hsa-miR-218-5p | DUSP5 | 1847 | ENSG00000138166 | predicted | 0.992 | |
diana_microt | MIMAT0002816 | hsa-miR-494-3p | SGMS2 | 166929 | ENSG00000164023 | predicted | 0.978 | |
diana_microt | MIMAT0002816 | hsa-miR-494-3p | INPP4B | 8821 | ENSG00000109452 | predicted | 0.889 | |
diana_microt | MIMAT0002816 | hsa-miR-494-3p | GATAD2B | 57459 | ENSG00000143614 | predicted | 0.85 | |
elmmo | MIMAT0000275 | hsa-miR-218-5p | TTC39C | 125488 | ENSG00000168234 | predicted | 0.712 | |
elmmo | MIMAT0000275 | hsa-miR-218-5p | SEMA4A | 64218 | ENSG00000196189 | predicted | 0.63 | |
elmmo | MIMAT0000275 | hsa-miR-218-5p | SORL1 | 6653 | ENSG00000137642 | predicted | 0.533 | |
elmmo | MIMAT0002816 | hsa-miR-494-3p | GATAD2B | 57459 | ENSG00000143614 | predicted | 0.505 | |
elmmo | MIMAT0002816 | hsa-miR-494-3p | SORL1 | 6653 | ENSG00000137642 | predicted | 0.504 | |
diana_microt | MIMAT0004767 | hsa-miR-193b-5p | PLXDC1 | 57125 | ENSG00000161381 | predicted | 0.831 |
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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
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Bonet, F.; Hernandez-Torres, F.; Ramos-Sánchez, M.; Quezada-Feijoo, M.; Bermúdez-García, A.; Daroca, T.; Alonso-Villa, E.; García-Padilla, C.; Mangas, A.; Toro, R. Unraveling the Etiology of Dilated Cardiomyopathy through Differential miRNA–mRNA Interactome. Biomolecules 2024, 14, 524. https://doi.org/10.3390/biom14050524
Bonet F, Hernandez-Torres F, Ramos-Sánchez M, Quezada-Feijoo M, Bermúdez-García A, Daroca T, Alonso-Villa E, García-Padilla C, Mangas A, Toro R. Unraveling the Etiology of Dilated Cardiomyopathy through Differential miRNA–mRNA Interactome. Biomolecules. 2024; 14(5):524. https://doi.org/10.3390/biom14050524
Chicago/Turabian StyleBonet, Fernando, Francisco Hernandez-Torres, Mónica Ramos-Sánchez, Maribel Quezada-Feijoo, Aníbal Bermúdez-García, Tomás Daroca, Elena Alonso-Villa, Carlos García-Padilla, Alipio Mangas, and Rocio Toro. 2024. "Unraveling the Etiology of Dilated Cardiomyopathy through Differential miRNA–mRNA Interactome" Biomolecules 14, no. 5: 524. https://doi.org/10.3390/biom14050524