Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes

Bermúdez, Miguel A.; Garrido, Alvaro; Pereira, Laura; Garrido, Teresa; Balboa, María A.; Balsinde, Jesús

doi:10.3390/biom14060707

Open AccessArticle

Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes

by

Miguel A. Bermúdez

^1,2,†

,

Alvaro Garrido

^1,2

,

Laura Pereira

^1,2,

Teresa Garrido

^1,2,

María A. Balboa

^2,3,*

and

Jesús Balsinde

^1,2,*

¹

Bioactive Lipids and Lipidomics Core, IBGM, CSIC-UVA, 47003 Valladolid, Spain

²

Centro de Investigación Biomédica en Red de Diabetes y Enfermedades Metabólicas Asociadas (CIBERDEM), Instituto de Salud Carlos III, 28029 Madrid, Spain

³

Lipid Metabolism and Inflammation Group, IBGM, CSIC-UVA, 47003 Valladolid, Spain

^*

Authors to whom correspondence should be addressed.

^†

Current address: Facultad de Ciencias y Artes, Universidad Católica de Avila (UCAV), 05005 Avila, Spain.

Biomolecules 2024, 14(6), 707; https://doi.org/10.3390/biom14060707

Submission received: 20 May 2024 / Revised: 10 June 2024 / Accepted: 13 June 2024 / Published: 15 June 2024

(This article belongs to the Collection Feature Papers in Lipids)

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Abstract

This work describes a novel route for phospholipid fatty acid remodeling involving the monounsaturated fatty acid palmitoleic acid. When administered to human monocytes, palmitoleic acid rapidly incorporates into membrane phospholipids, notably into phosphatidylcholine (PC). In resting cells, palmitoleic acid remains within the phospholipid pools where it was initially incorporated, showing no further movement. However, stimulation of the human monocytes with either receptor-directed (opsonized zymosan) or soluble (calcium ionophore A23187) agonists results in the rapid transfer of palmitoleic acid moieties from PC to phosphatidylinositol (PI). This is due to the activation of a coenzyme A-dependent remodeling route involving two different phospholipase A₂ enzymes that act on different substrates to generate free palmitoleic acid and lysoPI acceptors. The stimulated enrichment of specific PI molecular species with palmitoleic acid unveils a hitherto-unrecognized pathway for lipid turnover in human monocytes which may play a role in regulating lipid signaling during innate immune activation.

Keywords: palmitoleic acid; phospholipid remodeling; phospholipase A₂; inflammation; monocytes

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MDPI and ACS Style

Bermúdez, M.A.; Garrido, A.; Pereira, L.; Garrido, T.; Balboa, M.A.; Balsinde, J. Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes. Biomolecules 2024, 14, 707. https://doi.org/10.3390/biom14060707

AMA Style

Bermúdez MA, Garrido A, Pereira L, Garrido T, Balboa MA, Balsinde J. Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes. Biomolecules. 2024; 14(6):707. https://doi.org/10.3390/biom14060707

Chicago/Turabian Style

Bermúdez, Miguel A., Alvaro Garrido, Laura Pereira, Teresa Garrido, María A. Balboa, and Jesús Balsinde. 2024. "Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes" Biomolecules 14, no. 6: 707. https://doi.org/10.3390/biom14060707

APA Style

Bermúdez, M. A., Garrido, A., Pereira, L., Garrido, T., Balboa, M. A., & Balsinde, J. (2024). Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes. Biomolecules, 14(6), 707. https://doi.org/10.3390/biom14060707

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Rapid Movement of Palmitoleic Acid from Phosphatidylcholine to Phosphatidylinositol in Activated Human Monocytes

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