Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Reagents
2.3. Intravitreal Injection
2.4. Immunohistochemistry and Quantification of the RGC Number
2.5. Histological Evaluation of Optic Nerves and Quantification of Axon Numbers
2.6. Statistical Analyses
3. Results
3.1. Axonal Degeneration in the Optic Nerve by Intravitreal Injection of Rotenone
3.2. Axonal Degeneration of Retinal Ganglion Cells (RGCs) in the Retina by Intravitreal Injection of Rotenone
3.3. Rotenone-Induced RGC Soma Damage
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Group | Average (% of Vehicle) | SEM | |
---|---|---|---|
Day 3 | Vehicle | 100 | 9.8 |
Rotenone | 86.4 | 6.4 | |
Day 7 | Vehicle | 100 | 5.2 |
Rotenone | 16.3 | 0.3 |
Group | Average (% of Vehicle) | SEM | |
---|---|---|---|
Day 3 | Vehicle | 100 | 6 |
Rotenone | 68.7 | 9.6 | |
Day 7 | Vehicle | 100 | 14 |
Rotenone | 15.2 | 5.9 |
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Yamamoto, Y.; Taniguchi, T.; Shimazaki, A. Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats. Biomolecules 2024, 14, 1047. https://doi.org/10.3390/biom14091047
Yamamoto Y, Taniguchi T, Shimazaki A. Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats. Biomolecules. 2024; 14(9):1047. https://doi.org/10.3390/biom14091047
Chicago/Turabian StyleYamamoto, Yasuko, Takazumi Taniguchi, and Atsushi Shimazaki. 2024. "Rotenone-Induced Optic Nerve Damage and Retinal Ganglion Cell Loss in Rats" Biomolecules 14, no. 9: 1047. https://doi.org/10.3390/biom14091047