BMP2 Diminishes Angiotensin II-Induced Atrial Fibrillation by Inhibiting NLRP3 Inflammasome Signaling in Atrial Fibroblasts
Abstract
:1. Introduction
2. Methods
2.1. Patient Study
2.2. Animal Study
2.3. Cell Culture
2.4. mRNA Sequence
2.5. qRT-PCR
2.6. Western Blot
2.7. Histology
2.8. Elisa Assay
2.9. Statistical Analysis
3. Results
3.1. BMP2 Expression Upregulated in Different AF Model
3.2. BMP2 Attenuated Angiotensin II Induced-AF Inducibility via Preventing Atrial Fibrosis In Vivo
3.3. RNA-Sequence Identify the Negative Role of BMP2 in Rat Atrial Inflammation
3.4. Knockdown BMP2 Enhances NLRP3-Dependent Inflammasome Activation in Rat Atrial Fibroblast
3.5. BMP2 Prevents Ang II-Induced NLRP3 Inflammasome Signaling in Neonatal Rat Atrial Fibroblasts
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yuan, Y.; Zhang, H.; Xia, E.; Zhao, X.; Gao, Q.; Mu, H.; Liu, X.; Tian, Y.; Liu, L.; Shen, Q.; et al. BMP2 Diminishes Angiotensin II-Induced Atrial Fibrillation by Inhibiting NLRP3 Inflammasome Signaling in Atrial Fibroblasts. Biomolecules 2024, 14, 1053. https://doi.org/10.3390/biom14091053
Yuan Y, Zhang H, Xia E, Zhao X, Gao Q, Mu H, Liu X, Tian Y, Liu L, Shen Q, et al. BMP2 Diminishes Angiotensin II-Induced Atrial Fibrillation by Inhibiting NLRP3 Inflammasome Signaling in Atrial Fibroblasts. Biomolecules. 2024; 14(9):1053. https://doi.org/10.3390/biom14091053
Chicago/Turabian StyleYuan, Yue, Hang Zhang, Erwen Xia, Xinbo Zhao, Qiang Gao, Hongyuan Mu, Xingzuo Liu, Yuanye Tian, Lei Liu, Qiuling Shen, and et al. 2024. "BMP2 Diminishes Angiotensin II-Induced Atrial Fibrillation by Inhibiting NLRP3 Inflammasome Signaling in Atrial Fibroblasts" Biomolecules 14, no. 9: 1053. https://doi.org/10.3390/biom14091053