Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling
Abstract
:1. Introduction
2. Materials and Methods
2.1. Zebrafish Husbandy and Manipulation
2.2. Genomic Analysis
2.3. Whole-Mount In Situ Hybridization (WISH) and Neuromast Staining
2.4. Reverse Transcription–Polymerase Chain Reaction (RT-PCR)
2.5. In Vitro Transcription/Translation
2.6. Biochemical Studies
2.7. Quantitation and Statistical Analysis
3. Results
3.1. Expression of Socs4b
3.2. Targeted Mutation of Zebrafish socs4b
3.3. Phenotypic Analysis of Socs4b Mutants
3.4. Regulation of EGFR Signaling by Socs4b
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Trengove, M.; Rasighaemi, P.; Liongue, C.; Ward, A.C. Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling. Biomolecules 2024, 14, 1063. https://doi.org/10.3390/biom14091063
Trengove M, Rasighaemi P, Liongue C, Ward AC. Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling. Biomolecules. 2024; 14(9):1063. https://doi.org/10.3390/biom14091063
Chicago/Turabian StyleTrengove, Monique, Parisa Rasighaemi, Clifford Liongue, and Alister C. Ward. 2024. "Zebrafish Suppressor of Cytokine Signaling 4b (Socs4b) Is Dispensable for Development but May Regulate Epidermal Growth Factor Receptor Signaling" Biomolecules 14, no. 9: 1063. https://doi.org/10.3390/biom14091063