Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer
Abstract
:1. Introduction
2. Materials and Methods
2.1. Antibodies and Reagents
2.2. Human Samples
2.3. Mouse Samples
2.4. Cell Culture
2.5. Histological Analysis
2.6. In Situ Hybridization
2.7. Quantitative Real-Time PCR
2.8. Western Blot Analysis
2.9. Establishment of Syngeneic Mouse Tumor Models for In Vivo Bio Distribution Study of Pn Exon 21 Ab
2.10. Mouse Bio-Distribution Study of 89Zr-Labeled Pn-Ab
2.11. Statistics
3. Results
3.1. Periostin Splice Variant Expression Pattern in Adult Mice
3.2. Periostin Splice Variant Expression Patterns in Normal Human Tissues
3.3. Disease-Specific ASVs in Human Breast Cancer Tissue
3.4. In Vivo Bio Distribution Study of Pn-21Ab
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Patient No. | Age | Sex | T | N | M | Stage | HG | ER | PgR | HER2 |
---|---|---|---|---|---|---|---|---|---|---|
1 | 61 | F | 1 | 1 | 0 | IIA | 2 | + | - | - |
2 | 53 | F | 2 | 2 | 0 | IIIA | 2 | + | + | - |
3 | 46 | F | 2 | 1 | 0 | IIB | 1 | + | + | - |
4 | 38 | F | 1 | 0 | 0 | I | 2 | + | + | - |
5 | 79 | F | 2 | 0 | 0 | IIA | 1 | - | - | - |
6 | 66 | F | 1 | 1 | 0 | IIA | 3 | - | - | - |
7 | 74 | F | 1 | 0 | 0 | I | 1 | - | - | - |
8 | 77 | F | 1 | 0 | 0 | I | 3 | - | - | - |
9 | 67 | F | 1 | 0 | 0 | I | 3 | - | - | - |
10 | 46 | F | 1 | 0 | 0 | I | 2 | - | - | - |
11 | 56 | F | 1 | 0 | 0 | I | 3 | - | - | - |
12 | 66 | F | 1 | 0 | 0 | I | 2 | + | + | - |
Gene Name | Forward | Reverse |
---|---|---|
Mouse Pn 1 | ATAACCAAAGTCGTGGAACC | TGTCTCCCTGAAGCAGTCTT |
Mouse Pn 2 | CCATGACTGTCTATAGACCTG | TGTCTCCCTGAAGCAGTCTT |
Mouse Pn 3 | ATAACCAAAGTCGTGGAACC | TTTGCAGGTGTGTCTTTTTG |
Mouse Pn 4 | CCCCATGACTGTCTATAGACC | TTCTTTGCAGGTGTGTCTTTT |
Mouse 18s rRNA | ATGGCCGTTCTTAGTTGGTG | CGGACATCTAAGGGCATCAC |
Human Pn exon 3-4 | AAGGAATGAAAGGCTGCCCA | TCCAAGTTGTCCCAAGCCTC |
Human Pn exon 16-17 | TGTTCGTGGTAGCACCTTCAA | TGATAATAGGCTGAAGACTGCC |
Human Pn exon 21-22 | GGTCACCAAGGTCACCAAATTC | TGTTGGCTTGCAACTTCCTCAC |
Human 18s rRNA | CGGCTACCACATCCAAGGAA | CTGGAATTACCGCGGCT |
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Share and Cite
Kanemoto, Y.; Sanada, F.; Shibata, K.; Tsunetoshi, Y.; Katsuragi, N.; Koibuchi, N.; Yoshinami, T.; Yamamoto, K.; Morishita, R.; Taniyama, Y.; et al. Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer. Biomolecules 2024, 14, 1093. https://doi.org/10.3390/biom14091093
Kanemoto Y, Sanada F, Shibata K, Tsunetoshi Y, Katsuragi N, Koibuchi N, Yoshinami T, Yamamoto K, Morishita R, Taniyama Y, et al. Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer. Biomolecules. 2024; 14(9):1093. https://doi.org/10.3390/biom14091093
Chicago/Turabian StyleKanemoto, Yuko, Fumihiro Sanada, Kana Shibata, Yasuo Tsunetoshi, Naruto Katsuragi, Nobutaka Koibuchi, Tetsuhiro Yoshinami, Koichi Yamamoto, Ryuichi Morishita, Yoshiaki Taniyama, and et al. 2024. "Expression of Periostin Alternative Splicing Variants in Normal Tissue and Breast Cancer" Biomolecules 14, no. 9: 1093. https://doi.org/10.3390/biom14091093